Características biológicas de Boophilus microplus (acari: ixodidae) a partir de infestação experimental em cão

Franque, Marcos Pinheiro; Santos, Huarrisson Azevedo; Silva, Gil V.O. Da; Tajiri, Julio T.; Massard, Carlos Luiz

doi:10.1590/s1984-29612007000400011

Cited by 9 publications

(1 citation statement)

References 11 publications

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“…Rhipicephalus (B.) microplus is a one-host tick of cattle, and horses, caprines, and dogs are secondary hosts [ 9 , 10 ]. Consequently, parasitism in horses depends on the presence of cattle, the primary hosts, in the conditions of intercropped pastures in which both species are raised together [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Dynamics of Theileria equi Infection in Rhipicephalus (Boophilus) microplus during the Parasitic Phase in a Chronically Infected Horse

et al. 2022

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Experimental studies have demonstrated that Rhipicephalus (Boophilus) microplus transmits Theileria equi to horses. However, the degree and dynamics of this protozoan infection in the vector’s organism have not been fully elucidated. Therefore, this study aimed to evaluate the infection rate and parasitic load of T. equi in R. (B.) microplus, the infection dynamics in this arthropod during experimental infestation in a horse chronically infected with T. equi, and to evaluate the trans-stadial and intrastadial transmission competence of T. equi by R. (B.) microplus. The experimental infestation period of R. (B.) microplus on the horse was 33 days, but males were found on the animal up to 60 days post-infestation. After the fifth day post-infestation, ticks and equine blood were collected every two days. Whole ticks from the same developmental stage collected in the same day were pooled. Adult ticks were dissected to extract salivary glands and gut. DNA extraction was performed for all the samples, and they were then submitted to qPCRs for T. equi diagnosis. Freshly molted nymphs collected as larvae in the horse and freshly molted males and females collected as nymphs in the horse showed equal to or greater than 75% positivity for T. equi, indicating a strong possibility of trans-stadial transmission. The longest permanence of the male ticks on the horse associated with the high positivity rate of this type of sample for T. equi indicate that the male may play a role in the intrastadial transmission of T. equi to infection-free horses. The salivary glands displayed 77.78% positivity for T. equi and presented a higher infection rate at the end of the experimental period (100% from 29 to 33 days post-infection). This study shows that R. (B.) microplus has high T. equi infection rates and that the infection rate and parasitic load increased over the experimental period. These findings confirm the importance of chronically infected horses with T. equi as a source of infection for R. (B.) microplus.

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Section: Introductionmentioning

confidence: 99%

Dynamics of Theileria equi Infection in Rhipicephalus (Boophilus) microplus during the Parasitic Phase in a Chronically Infected Horse

et al. 2022

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Investigation of tick vectors of Hepatozoon canis in Brazil

Demoner

Rubini

Paduan

et al. 2013

Ticks and Tick-borne Diseases

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Hepatozoon spp. infections in wild rodents in an area of endemic canine hepatozoonosis in southeastern Brazil

Demoner

Magro

Silva

et al. 2016

Ticks and Tick-borne Diseases

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Hepatozoon canis is a tick-borne parasite that occurs worldwide. In rural areas of Brazil, H. canis vectors remain unknown, which has led to speculation about alternative routes of transmission. Small rodents can play a role in the transmission (via predation) of Hepatozoon americanum, which led us to question whether predation might be an alternative mode of transmission for H. canis. Thus, this study investigated whether Hepatozoon spp. are present in wild small rodents in forest fragments that surround rural areas in Botucatu County, São Paulo, Brazil, where canine hepatozoonosis is endemic. The study included blood samples from 158 dogs, which were screened by microscopy and molecular analysis. Blood samples and tissues from 67 rodents were obtained for histopathology and molecular detection. The prevalence of H. canis was high (66.45%) in dogs from rural areas of Botucatu, São Paulo, Brazil. The molecular analysis showed that wild rodent species in Brazil were infected with Hepatozoon spp. other than H. canis. Therefore, although the hypothesis that sylvatic rodents act as reservoirs for H. canis was not supported, the presence of monozoic cysts in the rodents suggests that, in addition to intermediate hosts, wild small rodents in Brazil might act as paratenic hosts of Hepatozoon spp. because they harbor infective stages for intermediate host predators.

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Características biológicas de Boophilus microplus (acari: ixodidae) a partir de infestação experimental em cão

Cited by 9 publications

References 11 publications

Dynamics of Theileria equi Infection in Rhipicephalus (Boophilus) microplus during the Parasitic Phase in a Chronically Infected Horse

Dynamics of Theileria equi Infection in Rhipicephalus (Boophilus) microplus during the Parasitic Phase in a Chronically Infected Horse

Investigation of tick vectors of Hepatozoon canis in Brazil

Hepatozoon spp. infections in wild rodents in an area of endemic canine hepatozoonosis in southeastern Brazil

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