Molecular characterization reveals Brazilian Tomato chlorosis virus to be closely related to a Greek isolate

Albuquerque, Leonardo Cunha de; Villanueva, Francisco; Resende, Renato O.; Navas‐Castillo, Jesús; Barbosa, J. C.; Inoue‐Nagata, Alice K.

doi:10.1590/s1982-56762013005000016

Cited by 17 publications

(14 citation statements)

References 10 publications

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“…This disease has rapidly spread across the main tomato production areas in Brazil (Barbosa et al, 2011;Macedo et al, 2014). ToCV is the only crinivirus that has been reported in tomatoes in Brazil (Barbosa et al, 2008(Barbosa et al, , 2011, and little genetic variability has been found among isolates (Albuquerque et al, 2013). Criniviruses belong to the family Closteroviridae, and their genomes are composed of two single-stranded positive-sense RNA molecules.…”

Section: Introductionmentioning

confidence: 99%

Temporal and spatial progress of the diseases caused by the crinivirus tomato chlorosis virus and the begomovirus tomato severe rugose virus in tomatoes in Brazil

et al. 2018

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Efficient management of whitefly‐borne diseases remains a challenge due to the lack of a comprehensive understanding of their epidemiology, particularly of the diseases tomato golden mosaic and tomato yellowing. Here, by monitoring 16 plots in four commercial fields, the temporal and spatial distribution of these two diseases were studied in tomato fields in Brazil. In the experimental plots these diseases were caused by tomato severe rugose virus (ToSRV) and tomato chlorosis virus (ToCV), respectively. The incidence of each virus was similar in the plots within a field but varied greatly among fields. Plants with symptoms for both diseases were randomly distributed in three of four spatial analyses. The curves representing the progress of both diseases were similar and contained small fluctuations, indicating that the spread of both viruses was similar under field conditions. In transmission experiments of ToSRV and ToCV by Bemisia tabaci MEAM1 (former biotype B), these viruses had a similar transmission rate in single or mixed infections. It was then shown that primary and secondary spread of ToCV were not efficiently controlled by insecticide applications. Finally, in a typical monomolecular model of disease progress, simulation of the primary dissemination of ToSRV and ToCV showed that infected plants were predominantly randomly distributed. It is concluded that, although the manner of vector transmission differs between ToSRV (persistent) and ToCV (semipersistent), the main dispersal mechanisms are most probably similar for these two diseases: primary spread is the predominant mechanism, and epidemics of these diseases have been caused by several influxes of viruliferous whiteflies.

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Section: Introductionmentioning

confidence: 99%

Temporal and spatial progress of the diseases caused by the crinivirus tomato chlorosis virus and the begomovirus tomato severe rugose virus in tomatoes in Brazil

et al. 2018

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“…In addition, the accession LA 444‐1 showed a high level of resistance to the begomovirus Tomato yellow vein streak virus (TYVSV) (Matos et al ., ), and the potyvirus Potato virus Y , strain PVY O (Lourenção et al ., ). Taking all these data together and considering the low genetic diversity of the ToCV Brazilian isolates based on the partial nucleotide sequence of the Hsp70h homologous gene (Barbosa et al ., ), as well as the high degree of nucleotide identity with isolates from other countries (Albuquerque et al ., ), it is believed that the lineage IAC‐CN‐RT might be of great value for tomato breeding programmes.…”

Section: Discussionmentioning

confidence: 99%

“…The ToCV isolate used in this study was obtained from a tomato plant in the region of Sumaré, state of São Paulo (Barbosa et al ., ), and the complete genome sequences for both RNAs are deposited in GenBank with accession numbers and (Albuquerque et al ., ). The virus isolate was maintained in tomato plants kept in an insect‐proof cage in the greenhouse.…”

Section: Methodsmentioning

confidence: 97%

Screening tomato genotypes for resistance and tolerance to Tomato chlorosis virus

et al. 2018

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Tomato chlorosis virus (ToCV) is an emerging crinivirus in Brazil that causes an economically important disease in tomato (Solanum lycopersicum) and other solanaceous species. ToCV is transmitted predominantly by the whitefly Bemisia tabaci Middle East‐Asia Minor 1 (MEAM1, formerly biotype B), in a semipersistent manner. As all cultivated tomato varieties and hybrids are susceptible to this crinivirus, the main alternatives for the control of the disease are the use of healthy seedlings for transplanting and the chemical control of the insect vector. The objective of this work was to evaluate the responses of tomato genotypes to infection with this crinivirus and their tolerance to the disease in order to support the development of other alternatives for disease control. Resistance to infection was evaluated by ToCV inoculation with viruliferous B. tabaciMEAM1 followed by virus detection by RT‐PCR and RT‐qPCR. To measure tolerance to the disease, plant development and fruit yield of ToCV‐infected and healthy plants were compared. Among 56 genotypes, only the lineage IAC‐CN‐RT (S. lycopersicum ‘Angela Gigante’ × S. peruvianum ‘LA 444‐1’) was highly resistant to infection with ToCV. Tolerance to the disease over two trials with different genotypes showed variable results. The effect of ToCV on plant development varied from 2.9% to 71.9% reduction, while yield loss varied from 0.2% to 51.8%. The highly ToCV‐resistant lineage IAC‐CN‐RT, which is also resistant to a Spanish isolate of ToCV, might be useful for tomato breeding programmes.

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“…To date, 17 complete ToCV genome sequences have been published, one each from the USA (Wintermantel et al , ), Spain (Lozano et al , , ), Greece (Kataya et al , ), Brazil (Albuquerque et al , ) and Taiwan (Kang et al , ), two from China (Zhao et al , , 2014b) and ten from Korea (Lee et al , ).…”

Section: Genome Organization and Diversitymentioning

confidence: 99%

Tomato chlorosis virus, an emergent plant virus still expanding its geographical and host ranges

Fiallo‐Olivé

Navas‐Castillo

2019

Molecular Plant Pathology

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Summary Tomato chlorosis virus (ToCV) causes an important disease that primarily affects tomato, although it has been found infecting other economically important vegetable crops and a wide range of wild plants. First described in Florida (USA) and associated with a ‘yellow leaf disorder’ in the mid‐1990s, ToCV has been found in 35 countries and territories to date, constituting a paradigmatic example of an emergent plant pathogen. ToCV is transmitted semipersistently by whiteflies (Hemiptera: Aleyrodidae) belonging to the genera Bemisia and Trialeurodes . Whitefly transmission is highly efficient and cases of 100% infection are frequently observed in the field. To date, no resistant or tolerant tomato plants are commercially available and the control of the disease relies primarily on the control of the insect vector. Taxonomy Tomato chlorosis virus is one of the 14 accepted species in the genus Crinivirus , one of the four genera in the family Closteroviridae of plant viruses. Virion and genome properties The genome of ToCV is composed of two molecules of single‐stranded positive‐sense RNA, named RNA1 and RNA2, separately encapsidated in long, flexuous, rod‐like virions. As has been shown for other closterovirids, ToCV virions are believed to have a bipolar structure. RNA1 contains four open reading frames (ORFs) encoding proteins associated with virus replication and suppression of gene silencing, whereas RNA2 contains nine ORFs encoding proteins putatively involved in encapsidation, cell‐to‐cell movement, gene silencing suppression and whitefly transmission. Host range In addition to tomato, ToCV has been found to infect 84 dicot plant species belonging to 25 botanical families, including economically important crops. Transmission Like all species within the genus Crinivirus , ToCV is semipersistently transmitted by whiteflies, being one of only two criniviruses transmitted by members of the genera Bemisia and Trialeurodes . Disease symptoms Tomato ‘yellow leaf disorder’ syndrome includes interveinal yellowing and thickening of leaves. Symptoms first develop on lower leaves and then advance towards the upper part of the plant. Bronzing and necrosis of the older leaves are accompanied by a decline in vigour and reduction in fruit yield. In other hosts the most common symptoms include interveinal chlorosis and mild yellowing on older leaves. Control Control of the disease caused by ToCV is based on the use of healthy seedlings for transplanting, limiting accessibility of alternate host plants that can serve as virus reservoirs and the spraying of insecticides for vector control. Although several wild tomato species have been sh...

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Molecular characterization reveals Brazilian Tomato chlorosis virus to be closely related to a Greek isolate

Cited by 17 publications

References 10 publications

Temporal and spatial progress of the diseases caused by the crinivirus tomato chlorosis virus and the begomovirus tomato severe rugose virus in tomatoes in Brazil

Temporal and spatial progress of the diseases caused by the crinivirus tomato chlorosis virus and the begomovirus tomato severe rugose virus in tomatoes in Brazil

Screening tomato genotypes for resistance and tolerance to Tomato chlorosis virus

Tomato chlorosis virus, an emergent plant virus still expanding its geographical and host ranges

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