Cytological aspects of the infection process of Pyricularia oryzae on leaves of wheat plants supplied with silicon

Sousa, Rodrigo Silva; Rodrigues, Fabrício Ávila; Schurt, Daniel Augusto; Souza, Naiara Fernandes Abreu; Cruz, Maria Fernanda Antunes

doi:10.1590/s1982-56762013000600002

Cited by 36 publications

(24 citation statements)

References 22 publications

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“…There was no difference between the leaf sections of the -Si and +Si non-inoculated plants. The present study confirms that Si can increase wheat resistance to blast (Xavier Filha et al, 2011;Sousa et al, 2013) and, to the best of our knowledge, provides novel evidence at the histochemical level about the involvement of this element in the potentiation of the biosynthetic pathway of flavonoids in the leaves of wheat plants infected with P. oryzae.…”

Section: Resultssupporting

confidence: 88%

Histochemical aspects of wheat resistance to leaf blast mediated by silicon

Silva

Cruz

Fortunato

et al. 2015

Sci. agric. (Piracicaba, Braz.)

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Blast, caused by Pyricularia oryzae, has become a significant disease threat to wheat (Triticum aestivum L.) in Brazil. This study aimed to investigate at the histochemical level if silicon (Si) could enhance the production of flavonoids in the leaves of wheat plants in response to P. oryzae infection. Plants from the Aliança cultivar, which are susceptible to blast, were grown in hydroponic cultures containing 0 (-Si) or 2 mM of Si (+Si) and inoculated by spraying a conidial suspension of P. oryzae (1 × 10 5 conidia mL ). This increased Si concentration was correlated with reduced fungal growth inside the epidermal cells and the development of blast symptoms on leaves. Strong fluorescence, which is an indication of the presence of flavonoids, was detected in the leaf cells of +Si plants usingNeu's and Wilson's reagents. A novel item of evidence is that, at the histochemical level, Si is involved in the potentiation of the biosynthetic pathway of flavonoids that increases wheat resistance to blast.

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Section: Resultssupporting

confidence: 88%

Histochemical aspects of wheat resistance to leaf blast mediated by silicon

Silva

Cruz

Fortunato

et al. 2015

Sci. agric. (Piracicaba, Braz.)

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“…v.73, n.2, p.177-183, March/April 2016 more similarities in their mechanisms and process of resistance (Gill et al, 2015). According to Rodrigues et al, (2005) and Sousa et al, (2013), in compatible rice-and wheat-P. oryzae interactions, fungal hyphae extensively penetrated and colonized the epidermal and mesophyll cells of the leaf tissue. Rodrigues et al, (2005) reported that in an incompatible rice-P. oryzae interaction, fungal hyphae were restricted to the first penetrated epidermal cell and appeared to have died at 48 hai due to the intense deposition of phenolic-like compounds.…”

Section: Discussionmentioning

confidence: 99%

Cytological aspects of incompatible and compatible interactions between rice, wheat and the blast pathogen Pyricularia oryzae

Araújo

Soares

Filippi

et al. 2016

Sci. agric. (Piracicaba, Braz.)

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Blast, caused by the fungus Pyricularia oryzae, is an important disease affecting rice and wheat yield worldwide. This study investigated the cytological aspects of incompatible (non-host resistance) and compatible (host resistance) rice-(R_Po) and wheat-(W_Po) Pyricularia oryzae isolate interactions. Inoculations of rice and wheat with the R_Po and W_Po isolates of P. oryzae, respectively, were expected to be compatible interactions (host resistance), whereas inoculations of rice and wheat with the W_Po and R_Po isolates of P. oryzae, respectively, were considered to be incompatible interactions (non-host resistance). For the compatible interactions (rice-R_Po and wheat-W_Po), fungal hyphae penetrated and colonized the epidermal cells and also invaded many neighboring cells. By contrast, in the case of the incompatible interactions (rice-W_Po and wheat-R_Po), fungal hyphae were not able to penetrate nor colonize the epidermal cells, but when penetration did occur, the hyphae were restricted to the first-invaded epidermal cell. The frequency of appressorial sites exhibiting infection hyphae within the epidermal cell underlying an appressorium was greater in the case of the compatible interactions. By contrast, unsuccessful penetrations with cytoplasmic granulation occurred with high frequency in the incompatible wheat-R_Po and rice-W_Po interactions and the number of necrotic epidermal cells underlying the appressorium was low for the rice-W_Po interaction as well as for the wheat-R_Po interaction, where no symptoms of necrosis were exhibited. However, the opposite was observed for the compatible interactions. The present study presents cytological features associated with incompatible and compatible rice-and wheat-P. oryzae interactions that may be useful to studies involving variability, coevolution, diagnosis, and regulation of quarantine or even in a rice or wheat breeding program whose aim is to transfer genes involved in non-host resistance to host resistance due to similarities in downstream mechanisms.

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“…Eletromicrografias de varredura de folhas de plantas de feijoeiro cultivadas em solução nutritiva na ausência (a e b) ou presença (c e d) de silício às 96 horas após inoculação com Colletotrichum lindemuthianum; barras a e c = 200 µm; b e d =100 µm. Sousa et al, 2013). De acordo com Polanco et al (2012), uma maior concentração de Si nas folhas de plantas de feijoeiro supridas com Si reduziu a área abaixo da curva do progresso da antracnose e contribuiu para o aumento do período de incubação.…”

Section: Discussionunclassified

“…Diante dessas preocupações, faz-se necessário encontrar métodos alternativos de controle da antracnose para diminuir as perdas ocasionadas por essa doença na cultura do feijoeiro. O silício (Si), apesar de não ser considerado um nutriente essencial às plantas, destaca-se por reduzir a intensidade de doenças importantes em várias culturas, principalmente gramíneas e algumas dicotiledôneas como café, pepino e soja (Cruz et al, 2012;Datnoff et al, 2007;Domiciano et al, 2013;Rodrigues et al, 2003;2005;Sousa et al, 2013). Na interação arroz-Pyricularia grisea, o aumento na resistência das plantas pela aplicação de Si foi associado com um aumento na densidade de células silicatadas na epiderme foliar ou à formação de uma espessa camada de sílica abaixo da cutícula, formando uma barreira física que impediu ou atrasou a penetração do fungo (Kim et al, 2004).…”

Section: Introductionunclassified

Aspectos microscópicos da interação feijoeiro-Colletotrichum lindemuthianum mediados pelo silício

et al. 2014

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A antracnose, causada pelo fungo Colletotrichum lindemuthianum, é uma das doenças mais destrutivas que afetam a cultura do feijoeiro. Com o objetivo de encontrar alternativas para o controle dessa doença, o presente trabalho investigou, em nível microscópico, o efeito do silício (Si) na resistência do feijoeiro à infecção por C. lindemuthianum. Plantas de feijoeiro (cv. Pérola) foram cultivadas em solução nutritiva contendo 0 (-Si) ou 2 mM (+Si) de Si e inoculadas no estádio de crescimento V4 com uma suspensão de conídios de C. lindemuthianum. A severidade da antracnose decresceu cerca de 52% nas folhas das plantas supridas com Si (4,4%) em relação às folhas das plantas não supridas (8,5%). Observações de folhas de feijoeiro das plantas não supridas com Si no microscópio eletrônico de varredura revelaram alterações morfológicas nas nervuras em contraste com as folhas de plantas supridas com Si. Utilizando-se a microanálise de raios-X, verificou-se maior concentração dos minerais enxofre, potássio e Si nas folhas das plantas supridas com Si. Em conclusão, o suprimento de Si em plantas de feijoeiro foi importante para reduzir os sintomas da antracnose.Palavras-chave: Colletotrichum lindemuthianum, Phaseolus vulgaris L., microscopia eletronica de varredura, microanálise de raios-X, nutrição mineral. Microscopic aspects of the interaction bean-Colletotrichum lindemuthianum mediated by silicon AbstractThe anthracnose, caused by the fungus Colletotrichum lindemuthianum, is one of the most destructive diseases affecting bean crop. With the objective to find alternative to the control this disease, this study investigated, at the microscopic level, the effect of silicon (Si) on bean resistance to infection by C. lindemuthianum. Bean plants (cv. 'Pérola') were grown in nutrient solution containing 0 (-Si) or 2 mM (+ Si) of Si and inoculated in the V4 growth stage with a suspension of conidia of C. lindemuthianum. The disease severity decreased by 52% in the leaves of plants supplied with Si (4.4%) in relation to the leaves of non supplied plants (8.5%). Observations of bean leaves of plants not supplied with Si by scanning electron microscopy revealed morphological changes in the veins in contrast with the leaves of plants supplied with Si. By using the X-ray microanalysis, it was found higher concentrations of the minerals sulfur, potassium and Si in the leaves of plants supplied with Si. In conclusion, the supply of Si in bean plants was important to reduce the symptoms of anthracnose.

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Cytological aspects of the infection process of Pyricularia oryzae on leaves of wheat plants supplied with silicon

Cited by 36 publications

References 22 publications

Histochemical aspects of wheat resistance to leaf blast mediated by silicon

Histochemical aspects of wheat resistance to leaf blast mediated by silicon

Cytological aspects of incompatible and compatible interactions between rice, wheat and the blast pathogen Pyricularia oryzae

Aspectos microscópicos da interação feijoeiro-Colletotrichum lindemuthianum mediados pelo silício

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