Evaluation of resistance to Tomato severe rugose virus (ToSRV) in Capsicum spp. genotypes

Abstract

Tomato severe rugose virus (ToSRV) is the predominant species of begomovirus in São Paulo State, Brazil, and infects primarily tomato and pepper plants. There is no information about genetic resistance of pepper to this virus, so in this work the reaction of 29 genotypes of Capsicum spp. was evaluated by inoculation of two ToSRV isolates: ToSRV-Sk (isolated from a tomato plant) and ToSRV-PJU (isolated from a pepper plant). For both isolates, two C. annuun genotypes (Catarino Cascabel -México and Silver) showed no symptoms 30 days after inoculation (d.a.i). In a second experiment, these two genotypes were evaluated for 150 d.a.i and, again, no symptoms could be observed. However, the virus was detected by RCA-PCR, indicating that both genotypes are susceptible, but less affected by ToSRV infection. Catarino Cascabel -México and Silver can be indicated for use in breeding programs for resistance of pepper to ToSRV.

Current status, breeding strategies and future prospects for managing chilli leaf curl virus disease and associated begomoviruses in Chilli (Capsicum spp.)

“…italica Plenk) plants. Rocha et al (2012) maintained non-viruliferous whiteflies on collard green (Brassica oleraceae var. acephala DC.)…”

Barcoding of Plant Viruses with Circular Single-Stranded DNA Based on Rolling Circle Amplification

“…A literature survey on the Web of Science for the years 2004 to 2018 yielded 146 papers in which RCA was mentioned explicitly for geminiviruses, mastreviruses, begomoviruses, curtoviruses, and nanoviruses [ 2 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 ,…”