Mancha bacteriana em Ruscus sp. causada por Burkholderia andropogonis no Brasil

Almeida, I. M. G.; Beriam, L. O. S.; Sannazzaro, A. M.; Neto, J. Rodrigues

doi:10.1590/s1982-56762009000500008

Cited by 3 publications

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“…A bactéria é Gram-negativa, as colônias apresentam coloração creme, crescimento lento e não são fluorescentes (LELLIOT; BILLING; HAYWARD, 1966;RODRIGUES NETO et al, 1981;SCHAAD;JONES;CHUN, 2001, ALMEIDA, et al, 2009.…”

Section: Robbsia Andropogonis (Lopes-santos L Et Al 2017)unclassified

“…eIto et al (2008), em cafeeiros expostos à ocorrência natural da doença, resultaram na seleção da cultivar IPR 102, tem se mostrado resistente a P. syringae pv. garcae (SERA; SERA; FAZUOLI, Pseudomonas cichorii, foi observado primeiramente em viveiros e campo no Estado de Minas Gerais(ROBBS et al, 1974) e em viveiro no Estado de São Paulo, com maior evidência em períodos mais chuvosos e temperaturas mais altas(ALMEIDA;PATRÍCIO, et al, 2012). Apresenta importância econômica pela ampla gama de plantas hospedeiras todo o mundo…”

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Desenvolvimento de metodologia de PCR multiplex para diagnose simultânea de Pseudomonas syringae pv. garcae e Pseudomonas syringae pv. tabaci e influência do nitrogênio no crescimento vegetativo do cafeeiro e severidade da mancha aureolada

Maciel¹

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The relevance of bacterial halo blight of coffee caused by Pseudomonas syringae pv. garcae, has increased recently in the coffee crop in Brazil. Other bacterioses also affect the coffee leaves and mixed infections of P. syringae pv. garcae and Pseudomonas syringae pv. tabaci have been found in 2017 in the state of Paraná. The techniques routinely used to diagnose these bacteria may spend resources and time. In the present study, the sequences of the genes dsbA, gyrB, rpoD, recA, rpoB, rpsLG and fusA. Only from the sequence of the rpoD gene allowed the development of two primers Psg3F for the detection of P. syringae pv. garcae, with 275 bp and a sensitivity of 0.001 ng, and Pst3F for the detection of P. syringae pv. tabaci, with 585 bp, and a sensitivity of 0.01 ng. These primes can be used simultaneously in a Multiplex PCR reaction to simultaneously to detect both pathogens, from extracted DNA and from boiled bacterial suspensions. This technique allows greater precision and reduction in the time used for diagnosis. The severity of the bacterial halo blight has been related to variations in the nitrogen nutrition of the coffee crop in field and nursery conditions. Aiming to estimate this influence, two experiments were carried out in hydroponic conditions with coffee seedlings of susceptible (Bourbon and Catuaí Vermelho IAC 144) and resistant (IPR 102) cultivars. The seedlings were grown in nutrient solutions containing 0, 5, 10 e 15 mmol L-1 of nitrogen (N) and were inoculated were with P. syringae pv. garcae. The seedlings presented a higher level of N and high growth of the aerial part, and dry mass of the aerial part, as well as a higher number of leaves, in response to the N supply. The seedlings of the susceptible cultivars presented more severity of bacterial halo blight in the solution without N, reduction in the severity in the solutions 5 and 10 mnol of N.L-1 and increase in the severity in the solution containing 15 mnol of N.L-1. The seedlings of the cultivar IPR 102 maintained resistance to bacterial halo blight in all treatments.

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Section: Robbsia Andropogonis (Lopes-santos L Et Al 2017)unclassified

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Desenvolvimento de metodologia de PCR multiplex para diagnose simultânea de Pseudomonas syringae pv. garcae e Pseudomonas syringae pv. tabaci e influência do nitrogênio no crescimento vegetativo do cafeeiro e severidade da mancha aureolada

Maciel¹

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Reassessment of the taxonomic position of Burkholderia andropogonis and description of Robbsia andropogonis gen. nov., comb. nov.

Lopes-Santos

Castro

Ferreira-Tonin

et al. 2017

Antonie van Leeuwenhoek

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The phylogenetic classification of the species Burkholderia andropogonis within the Burkholderia genus was reassessed using 16S rRNA gene phylogenetic analysis and multilocus sequence analysis (MLSA). Both phylogenetic trees revealed two main groups, named A and B, strongly supported by high bootstrap values (100%). Group A encompassed all of the Burkholderia species complex, whi.le Group B only comprised B. andropogonis species, with low percentage similarities with other species of the genus, from 92 to 95% for 16S rRNA gene sequences and 83% for conserved gene sequences. Average nucleotide identity (ANI), tetranucleotide signature frequency, and percentage of conserved proteins POCP analyses were also carried out, and in the three analyses B. andropogonis showed lower values when compared to the other Burkholderia species complex, near 71% for ANI, from 0.484 to 0.724 for tetranucleotide signature frequency, and around 50% for POCP, reinforcing the distance observed in the phylogenetic analyses. Our findings provide an important insight into the taxonomy of B. andropogonis. It is clear from the results that this bacterial species exhibits genotypic differences and represents a new genus described herein as Robbsia andropogonis gen. nov., comb. nov.

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Burkholderia andropogonis (bacterial leaf stripe of sorghum and corn)

2022

CABI Compendium

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Mancha bacteriana em Ruscus sp. causada por Burkholderia andropogonis no Brasil

Cited by 3 publications

References 10 publications

Desenvolvimento de metodologia de PCR multiplex para diagnose simultânea de Pseudomonas syringae pv. garcae e Pseudomonas syringae pv. tabaci e influência do nitrogênio no crescimento vegetativo do cafeeiro e severidade da mancha aureolada

Desenvolvimento de metodologia de PCR multiplex para diagnose simultânea de Pseudomonas syringae pv. garcae e Pseudomonas syringae pv. tabaci e influência do nitrogênio no crescimento vegetativo do cafeeiro e severidade da mancha aureolada

Reassessment of the taxonomic position of Burkholderia andropogonis and description of Robbsia andropogonis gen. nov., comb. nov.

Burkholderia andropogonis (bacterial leaf stripe of sorghum and corn)

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