Packaging properties and control of Listeria monocytogenes in bologna by cellulosic films incorporated with pediocin

Abstract

Listeria monocytogenes is a foodborne pathogen, able to survive and proliferate at refrigeration temperatures. As a result, ready-to-eat meat products have been associated with major outbreaks. Producing meat products involves lethal preservation treatments, e.g. thermal treatments. Listeria contamination, however, may be introduced when products are sliced and packaged at retail businesses or delicatessens. In Brazil, sliced bologna is very popular at retail markets. After slicing, however, bologna has a short shelf-life. The aim of this work was to study the effects of pediocin incorporation on the load at break, water vapor permeability rate and structure, by microscopic analysis, of antimicrobial cellulosic packaging. The potential application of the developed packaging for the preservation of bologna and inhibition of Listeria biofilm formation was also studied. Cellulosic antimicrobial packaging films were produced with cellulose acetate and acetone. Pediocin (commercially available concentrate ALTA TM 2341) was incorporated at 30, 40 and 50 % w/w. The load at break of films was studied using the Universal Testing Machine (Instron) at 10 °C and 25 °C. The water vapor permeability was determined by gravimetric method. A scanning electron microscope was used to study the developed packaging structure. Antimicrobial activity of films against Listeria innoucua and L. monocytogenes was tested both in vitro and in bologna samples. Results showed that values of load at break decreased with increasing concentrations of pediocin at 10 °C and 25 °C. Regarding water vapor permeability, only the control and 50 % pediocin films presented statistical difference, with the 50 % pediocin film being more permeable. In vitro tests showed antimicrobial activity against L. innocua. Cellulosic film with 50 % pediocin reduced L. monocytogenes growth on sliced bologna by 1.2 log cycles after 9 days and prevented biofilm formation on packaging and bologna surfaces. Hence, active cellulosic films made with 50 % pediocin in the form of commercially available concentrated ALTA™ 2341 have the potential of being used in a system of hurdle technologies as a final obstacle for L. monocytogenes control in bologna preservation.

Cellulose nanofiber (CNF)–sakacin‐A active material: production, characterization and application in storage trials of smoked salmon

“…Saini et al developed a novel antimicrobial film with covalently linked nisin on surface of TEMPO oxidized cellulose nanofibers for food packaging. Espitia et al studied the effects of pediocin incorporation into a cellulosic packaging produced with cellulose acetate resin, determining the tensile strength at break (in MPa), load at break (in newtons) and elongation at break (%), water vapor permeability and structure. Santiago‐Silva et al developed and evaluated the antimicrobial effect of cellulose acetate matrix films incorporated with pediocin on the preservation of samples of sliced ham.…”

Antagonistic Activity and Surface Decontaminant Potential of Lactic Acid Bacteria from Fermented Oreochromis niloticus

“…The application of pure pediocin also might be the most effective for the Tilapia fillet. The effectiveness of treatment using pediocin has been anticipated by several researchers, who verified its ex situ efficiency by applying it to a variety of meat, against numerous contaminant species and foodborne pathogens (Chen & Hoover, 2003;Santiago-Silva et al, 2009;Espitia et al, 2013;da Silva Sabo et al, 2017).…”

Antibacterial Activity of Pediocin and Pediocin-Producing Bacteria Against Listeria monocytogenes in Meat Products

“…They found that L. monocytogenes growth was completely prevented in the samples coated with plastic packaging bags containing pediocin powder during 12 weeks of storage at 4 • C. In addition, Woraprayote et al (2013) observed that L. monocytogenes was decreased by 2 log cfu/g in the raw sliced pork that was packed with poly(lactic acid)/sawdust particle biocomposite film incorporated with pediocin PA-1/AcH. Similarly, Santiago- Silva et al (2009) reported a 2 log cfu/ml reduction on the sliced ham coated with cellulose-based film impregnated with 50% pediocin during 15 days of storage at 12 • C. Likewise, it has been observed that application of commercially available pediocin (ALTA TM 2341) in the cellulosic film-forming solution at concentrations of 50% (w/w) resulted in a 1.2 log cfu/g reduction in L. monocytogenes population on sliced bologna and hindered biofilm formation on packaging and bologna surfaces (Espitia et al, 2013). Therefore, it seems that pediocin could be a packaging material, as part of the hurdle technology system for the barrier of L. monocytogenes in meat and meat products.…”