Accuracy and reliability of a videogrammetry system in the analysis of displacement of Wistar rats

Abstract

-Aims:The behavior of laboratory animals has been studied through displacement, with different objectives by researchers. Methods: Although different methods have already been used in the tracking of laboratory animals, manual tracking mode videogrammetry for 2D analysis of displacement has not been observed in animal studies. The aim of this study was to verify the accuracy and reliability of determining the displacement of Wistar rats by means of videogrammetry software Dvideo. The accuracy (between the known distance and distance traced) was determined by 3 different evaluators twice consecutive, by videoing a course of 10 meters in the enriched environment together with further analysis of the displacement of the midpoint marked on an apparatus. To calculate reliability (accuracy of the measurement system) and reproducibility (precision of the evaluators) to obtain the ratio of precision to tolerance (P/T), eight animals were filmed for 10 minutes in the enriched environment and analysis of distance covered by one of the animals by three different reviewers thrice consecutive. Results: The results obtained in the course of 10 meters of the known distance demonstrated accuracy of 0.10 m, precision of 0.05 m, and bias of 0.07 m. In the reliability test during the 10 minutes of displacement (m) of animal, the ratio of precision to tolerance (P/T) = 0.1 m was found between three different evaluators, demonstrating adequate capacity of the measure. Conclusion: The manual tracking mode of the Dvideo presented high reliability and it can be employed for the displacement analysis of studies with rat experimental models.

Incerteza de medição na avaliação de desempenho de métodos analíticos e estratégias de melhoria com a aplicação do Analytical Quality by Design

“…Razões iguais ou menores que 10% indicam adequada capacidade de medição, portanto, que o método é aplicável ao seu propósito 47,63 . Um dos guias da Eurachem/Citac 64 recomenda razão de 25%, ou U alvo de 2,5% para uma especificação de 90 a 110%, reconhecendo que valores mais baixos ou mais altos podem ser muito restritos ou flexíveis.…”