Histological Markers of Degeneration and Regeneration of the Human Intervertebral Disk

Peletti-Figueiró, Manuela; Aguiar, Israel Silveira De; Paesi, Suelen; Echeverrigaray, Sérgio; Roesch‐Ely, Mariana; Falavigna, Asdrúbal; Henriques, João Antônio Pêgas

doi:10.1590/s1808-185120171601170833

Cited by 4 publications

(4 citation statements)

References 30 publications

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“…The cellular morphology of the BMSC/TGF group was very similar to the Normal group, suggesting that h BMSCs and TGF-β3 can effectively promote regeneration of IVD. The degree of regeneration was evaluated by the number of chondrocyte clusters (an indicator of IVD degeneration) per unit area (500 μm × 500 μm) . The Microdiscectomy group exhibited a higher number of cell clusters (39.7 ± 2.5) than other groups, indicating severe IVD degeneration (Figure D).…”

Section: Resultsmentioning

confidence: 99%

“…The degree of regeneration was evaluated by the number of chondrocyte clusters (an indicator of IVD degeneration) per unit area (500 μm × 500 μm). 85 The Microdiscectomy group exhibited a higher number of cell clusters (39.7 ± 2.5) than other groups, indicating severe IVD degeneration (Figure 7D). The degree of IVD regeneration among the experimental groups was as follows: BMSC/TGF (3.0 ± 0.8 cell clusters) > TGF (6.3 ± 0.5) > BMSC (9.7 ± 0.5).…”

Section: ■ Experimental Sectionmentioning

confidence: 92%

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Intervertebral Disc Regeneration Using Stem Cell/Growth Factor-Loaded Porous Particles with a Leaf-Stacked Structure

et al. 2020

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Although biological therapies based on growth factors and transplanted cells have demonstrated some positive outcomes for intervertebral disc (IVD) regeneration, repeated injection of growth factors and cell leakage from the injection site remain considerable challenges for human therapeutic use. Herein, we prepare human bone marrow-derived mesenchymal stem cells (hBMSCs) and transforming growth factor-β3 (TGF-β3)-loaded porous particles with a unique leaf-stack structural morphology (LSS particles) as a combination bioactive delivery matrix for degenerated IVD. The LSS particles are fabricated with clinically acceptable biomaterials (polycaprolactone and tetraglycol) and procedures (simple heating and cooling). The LSS particles allow sustained release of TGF-β3 for 18 days and stable cell adhesiveness without additional modifications of the particles. On the basis of in vitro and in vivo studies, it was observed that the hBMSCs/TGF-β3-loaded LSS particles can provide a suitable milieu for chondrogenic differentiation of hBMSCs and effectively induce IVD regeneration in a beagle dog model. Thus, therapeutically loaded LSS particles offer the promise of an effective bioactive delivery system for regeneration of various tissues including IVD.

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Section: Resultsmentioning

confidence: 99%

Section: ■ Experimental Sectionmentioning

confidence: 92%

Intervertebral Disc Regeneration Using Stem Cell/Growth Factor-Loaded Porous Particles with a Leaf-Stacked Structure

et al. 2020

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“…Immunopositivity for collagen I and III expression were assessed on paraffin tissue sections (3-µm thick) by using polyclonal rabbit anti-collagen I (1:100, Cat #ab34710, abcam, Cambridge, United Kingdom), and polyclonal rabbit anti-collagen III (1:50, #ab7778, abcam, Cambridge, United Kingdom), according to the procedures previously described [17]. The samples were treated with an amplifier (HiDef Detection Amplifier Mouse & Rabbit-Cell Marque) followed by the detection polymer (HiDef Detection HRP Polymer Detector-Cell Marque).…”

Section: Immunohistochemistry (Evalution Of Collagen I and Iii)mentioning

confidence: 99%

“…The samples were treated with an amplifier (HiDef Detection Amplifier Mouse & Rabbit-Cell Marque) followed by the detection polymer (HiDef Detection HRP Polymer Detector-Cell Marque). The materials were stained with DAB (Diaminobenzidine-Cell Marque) and counterstained with Harris Hematoxylin [17]. Images were examined with a Leica DM2500 optical microscope (Leica Microsystems, Bannockburn, IL, U.S.A.).…”

Section: Immunohistochemistry (Evalution Of Collagen I and Iii)mentioning

confidence: 99%

Towards on the Mediators in Collagen Replacement: Effect of the Active Molecules into a Polymeric Biomaterial

Ingracio¹,

Nicoletti²,

Dias³

et al. 2021

JBM

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Modulating healing factors could avoid or minimize some possible pathological processes in collagen deposition. The present study was aimed to evaluated the role of active biomolecules such as PDGF-BB and PRP loaded or not into polymeric biomaterial to seek potential mediators in types I and III collagen deposition and epithelization. The healing phases were investigated by using an in vivo full-thickness wound rat model. At zero, 3 rd , 7 th and 14 th days after the experimental model, the size of the wound areas was photographed. The nanofibrous materials were biocompatible and did not cause any local adverse reaction and/or inflammation. On day 14 the wounds had healed almost 100% with better signs of healing, however there was no obvious difference in the wound contraction rates. At the end of 14 days, samples from the center of the lesion were collected when histological features and immunopositivity for collagen I and III expressions were assessed. There was no significant difference in the epithelization among the groups. Wounds treated with PRP and with PA-6/SOMA plus PDGF-BB had significantly lower amounts of type III collagen. The amounts of type I collagen did not have a statistically different deposition among the experimental groups. The association of PDGF-BB with PA-6/SOMA emerges as an alternative for topical application to unfavorable anatomical sites, suggesting that these associations may have a positive modulation on the process of accelerated healing remodeling.

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Kinesiotaping in Discopathies and Degenerative Spinal Diseases: Systematic Review

et al. 2023

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Neuromuscular taping or kinesiotaping is a technique widely used in spinal disorders. However, the scientific evidence of its use in discopathies and degenerative spine pathology is unknown. This study aimed to analyze the published clinical trials on neuromuscular taping in subjects with discopathies and degenerative spinal injuries. For this purpose, a literature search was performed following PRISMA guidelines in the following databases: PubMed, Web of Science (WOS), Scopus, Medline, and Cinahl. In analyzing bias and methodological quality, we used: the PEDro scale, Van Tulder criteria, and risk of bias analysis of the Cochrane Collaboration. A total of 5 articles were included that obtained a mean score of 6.2 on the PEDro scale. There is moderate evidence that, in the short term, neuromuscular taping reduces analgesic consumption and improves the range of motion and muscle strength in the posterior musculature. In addition, there is limited evidence that it can improve quality, while the scientific evidence on the effect of neuromuscular taping on pain is contradictory. The application of neuromuscular taping on discopathies and degenerative processes of the spine should be cautiously undertaken until more conclusive results are obtained, and the long-term effects are assessed. Level of evidence I; Systematic review.

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Histological Markers of Degeneration and Regeneration of the Human Intervertebral Disk

Cited by 4 publications

References 30 publications

Intervertebral Disc Regeneration Using Stem Cell/Growth Factor-Loaded Porous Particles with a Leaf-Stacked Structure

Intervertebral Disc Regeneration Using Stem Cell/Growth Factor-Loaded Porous Particles with a Leaf-Stacked Structure

Towards on the Mediators in Collagen Replacement: Effect of the Active Molecules into a Polymeric Biomaterial

Kinesiotaping in Discopathies and Degenerative Spinal Diseases: Systematic Review

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