Adipocyte morphometric evaluation and angiogenesis in the omentum transposed to the breast: a preliminary study

Abstract

OBJECTIVE:The purpose of this study was to describe the probable mechanism of the volume increase of laparoscopically harvested omentum flaps used to treat breast deformities.METHODS:A histological analysis of omentum samples was performed to study the volume increase of laparoscopically harvested omentum flaps. Samples were harvested immediately after the transposition of the omentum from the abdominal cavity to the breast region and during the second surgical procedure for breast symmetrization of eight patients submitted to the transposition of the omentum flap. Changes in the morphometric measurements of the adipocytes (perimeter, diameter, and area), microvascular density (as measured by the CD31 endothelial marker), and immunohistochemical expression of VEGF were documented.RESULTS:The increases in adipocyte size and microvascular density were statistically significant (P ≤ 0.012). The expression levels of VEGF were lower in the second set of samples when compared to the first set, but the differences were not statistically significant (P < 0.093).CONCLUSION:These results demonstrate an increase in cellular volume as measured by adipocyte perimeter, diameter, and area. Moreover, the increase in the number of vessels in the second set of samples suggests that neoangiogenesis was stimulated by the initial increase in VEGF expression levels observed in the first set of samples. The increase in VEGF expression in the flap may have been caused by adipocyte hypertrophy resulting from neoangiogenesis.

Comparative Study of Morphological Characteristics and Body Composition between Different Team Players from Serbian Junior National League: Soccer, Handball, Basketball and Volleyball

“…An important decline of fat levels can lead to complications and contraindications. Adipose tissue is a complex, essential, and highly active metabolic and endocrine organ (Kershaw & Flier, 2004) who communicates with the central nervous system (Costa et al, 2011). In addition to energy storage, also regulate energy metabolism via secreting adipokines into the circulation (Ozenoglu et al, 2008).…”

The Omentum Flap

“…This difference ranged from a big to a very big effect. The confidence intervals of these effect sizes were always above moderate (Costa et al, 2011).…”

“…In the first six months after the procedure the omentum flap presents a variable growth that needs to be considered when planning repair of the deformity Costa et al, 2011).…”

DMPE-PEG scaffold binding with TGF-β1 receptor enhances cardiomyogenic differentiation of adipose-derived stem cells

“…Unlike most tissues, adipose tissue is highly malleable in adults due to the hypertrophy of adipocytes. Previous studies suggest that the proliferative hypertrophy of adipocytes results from the existence of a special type of progenitor cell, namely the pre-adipocyte [16]. Additionally, researchers have demonstrated that there indeed exists a type of adipose-derived stem cells (ADSCs), which has strong regeneration and differentiation potentials in vitro.…”