Cardioprotection conferred by exercise training is blunted by blockade of the opioid system

Galvão, Tatiana; Matos, Katt C; Brum, Patrícia Chakur; Negräo, Carlos Eduardo; Luz, Protásio Lemos da; Chagas, Antônio Carlos Palandri

doi:10.1590/s1807-59322011000100026

Cited by 23 publications

(22 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They found that both the stretched muscle mass and the VM influence acute cardiovascular responses to multiple-set passive stretching exercise sessions. Galvão et al [29] employed a murine model to investigate the effect of opioid receptor blockade on the myocardial protection conferred by chronic exercise and to compare exercise training with different strategies of myocardial protection (opioid infusion and brief periods of ischemia-reperfusion) preceding irreversible left anterior descending coronary ligation. They found that exercise training, morphine, exercise training plus morphine, and ischemia-reperfusion groups had a smaller infarct area than the control group.…”

Section: Kk and Rocha E Silva M -Cardiology In Brazilian Scientific Joumentioning

confidence: 99%

Cardiology in Brazilian scientific journals: an overview

Patel

Silva

2012

Rev Bras Cir Cardiovasc

View full text Add to dashboard Cite

Cardiology has been and is a very significant fraction of the Brazilian contribution to science. In older days, the most significant part of this work was directed to foreign periodicals, but the quasi-simultaneous emergence of SciELO and PUBMED has ordained a redirection of much of this work to Brazilian periodicals. We here survey some of this more recent contribution for the benefit of readers of Revista Brasileira de Cirurgia Cardiovascular. This is offered as an update. Articles on the general themes of Cardiology and Pneumology published by four ISI Indexed Brazilian journals not specializing in cardiology are re-visited, after a search through 10 journals. ResumoA Cardiologia foi, e continua sendo, responsável por uma fracção muito significativa da contribuição brasileira para a ciência. No passado, os trabalhos mais importantes eram direcionados para periódicos estrangeiros, mas o surgimento quase simultâneo do SciELO e PUBMED facilitou um redirecionamento de grande parte desses trabalhos a periódicos brasileiros. Neste artigo, examinamos algumas das contribuições mais recentes em benefício aos leitores da Revista Brasileira de Cirurgia Cardiovascular. Esta discussão é oferecida como uma atualização. Artigos sobre os temas gerais da cardiologia e pneumologia publicados por quatro revistas brasileiras indexadas no ISI -não especializadas em cardiologia -são revistos.RBCCV

show abstract

Section: Kk and Rocha E Silva M -Cardiology In Brazilian Scientific Joumentioning

confidence: 99%

Cardiology in Brazilian scientific journals: an overview

Patel

Silva

2012

Rev Bras Cir Cardiovasc

View full text Add to dashboard Cite

show abstract

“…Rather, SLP induces unconventional transcriptional changes, including shifts in mediators of inflammation/immunity, sarcomere function, and cardiovascular growth and development ( Figures 3 and 4 , Table S1 ). Intriguingly, this response exhibits features similar to those arising with cardioprotective exercise [17], itself attributed to OR-dependent signaling [18], [19]. Both SLP and voluntary-running generate predominant mRNA repression vs .…”

Section: Discussionmentioning

confidence: 82%

Unique Transcriptional Profile of Sustained Ligand-Activated Preconditioning in Pre- and Post-Ischemic Myocardium

et al. 2013

View full text Add to dashboard Cite

BackgroundOpioidergic SLP (sustained ligand-activated preconditioning) induced by 3–5 days of opioid receptor (OR) agonism induces persistent protection against ischemia-reperfusion (I-R) injury in young and aged hearts, and is mechanistically distinct from conventional preconditioning responses. We thus applied unbiased gene-array interrogation to identify molecular effects of SLP in pre- and post-ischemic myocardium.Methodology/Principal FindingsMale C57Bl/6 mice were implanted with 75 mg morphine or placebo pellets for 5 days. Resultant SLP did not modify cardiac function, and markedly reduced dysfunction and injury in perfused hearts subjected to 25 min ischemia/45 min reperfusion. Microarray analysis identified 14 up- and 86 down-regulated genes in normoxic hearts from SLP mice (≥1.3-fold change, FDR≤5%). Induced genes encoded sarcomeric/contractile proteins (Myh7, Mybpc3,Myom2,Des), natriuretic peptides (Nppa,Nppb) and stress-signaling elements (Csda,Ptgds). Highly repressed genes primarily encoded chemokines (Ccl2,Ccl4,Ccl7,Ccl9,Ccl13,Ccl3l3,Cxcl3), cytokines (Il1b,Il6,Tnf) and other proteins involved in inflammation/immunity (C3,Cd74,Cd83, Cd86,Hla-dbq1,Hla-drb1,Saa1,Selp,Serpina3), together with endoplasmic stress proteins (known: Dnajb1,Herpud1,Socs3; putative: Il6, Gadd45g,Rcan1) and transcriptional controllers (Egr2,Egr3, Fos,Hmox1,Nfkbid). Biological themes modified thus related to inflammation/immunity, together with cellular/cardiovascular movement and development. SLP also modified the transcriptional response to I-R (46 genes uniquely altered post-ischemia), which may influence later infarction/remodeling. This included up-regulated determinants of cellular resistance to oxidant (Mgst3,Gstm1,Gstm2) and other forms of stress (Xirp1,Ankrd1,Clu), and repression of stress-response genes (Hspa1a,Hspd1,Hsp90aa,Hsph1,Serpinh1) and Txnip.ConclusionsProtection via SLP is associated with transcriptional repression of inflammation/immunity, up-regulation of sarcomeric elements and natriuretic peptides, and modulation of cell stress, growth and development, while conventional protective molecules are unaltered.

show abstract

“…This exercise training protocol was selected because this training program has been shown to promote cardioprotection against IR injury [21]. The exercise training occurred between 8:00 and 10:00 am each day.…”

Section: Methodsmentioning

confidence: 99%

Delta Opioid Receptors: The Link between Exercise and Cardioprotection

et al. 2014

View full text Add to dashboard Cite

This study investigated the role of opioid receptor (OR) subtypes as a mechanism by which endurance exercise promotes cardioprotection against myocardial ischemia-reperfusion (IR) injury. Wistar rats were randomly divided into one of seven experimental groups: 1) control; 2) exercise-trained; 3) exercise-trained plus a non-selective OR antagonist; 4) control sham; 5) exercise-trained plus a kappa OR antagonist; 6) exercise-trained plus a delta OR antagonist; and 7) exercise-trained plus a mu OR antagonist. The exercised animals underwent 4 consecutive days of treadmill training (60 min/day at ∼70% of maximal oxygen consumption). All groups except the sham group were exposed to an in vivo myocardial IR insult, and the myocardial infarct size (IS) was determined histologically. Myocardial capillary density, OR subtype expression, heat shock protein 72 (HSP72) expression, and antioxidant enzyme activity were measured in the hearts of both the exercised and control groups. Exercise training significantly reduced the myocardial IS by approximately 34%. Pharmacological blockade of the kappa or mu OR subtypes did not blunt exercise-induced cardioprotection against IR-mediated infarction, whereas treatment of animals with a non-selective OR antagonist or a delta OR antagonist abolished exercise-induced cardioprotection. Exercise training enhanced the activities of myocardial superoxide dismutase (SOD) and catalase but did not increase the left ventricular capillary density or the mRNA levels of HSP72, SOD, and catalase. In addition, exercise significantly reduced the protein expression of kappa and delta ORs in the heart by 44% and 37%, respectively. Together, these results indicate that ORs contribute to the cardioprotection conferred by endurance exercise, with the delta OR subtype playing a key role in this response.

show abstract

Cardioprotection conferred by exercise training is blunted by blockade of the opioid system

Cited by 23 publications

References 33 publications

Cardiology in Brazilian scientific journals: an overview

Cardiology in Brazilian scientific journals: an overview

Unique Transcriptional Profile of Sustained Ligand-Activated Preconditioning in Pre- and Post-Ischemic Myocardium

Delta Opioid Receptors: The Link between Exercise and Cardioprotection

Contact Info

Product

Resources

About