Effects of Pentoxifylline on TNF-Alpha and Lung Histopathology in HCl-Induced Lung Injury

Oliveira-Júnior, Itamar Souza; Maganhin, Carla Cristina; Carbonel, Adriana Aparecida Ferraz; Monteiro, Cristina M.; Cavassani, Sâmia Santos; Oliveira‐Filho, Ricardo Martins

doi:10.1590/s1807-59322008000100014

Cited by 15 publications

(13 citation statements)

References 34 publications

(27 reference statements)

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“…Animal studies have demonstrated improved oxygenation and ventilation perfusion ratios, along with restoring aeration, decreasing shunt, and preserving perfusion 1,9,15 . In addition, prone positioning has been shown to eliminate compression of the lungs by the heart 16 .…”

Section: Discussionmentioning

confidence: 99%

“…A hind paw vein (used for drug infusions and compensation of fluid losses) and the left internal carotid artery (used to obtain arterial blood for gases determination) were exposed and cannulated using a PE-50 tubing (Intramedic Polyethylene Tubing, Becton Dickinson, NJ, USA). Normothermia was maintained with an underlying electric hotplate (37 ± 0.6oC [mean±SD]) 1 .…”

Section: Surgical Proceduresmentioning

confidence: 99%

“…A venous catheter was placed in the jugular vein to allow the infusion of isotonic saline solution and sodium pentobarbital whenever needed 1 .…”

Section: Mechanical Ventilation (Mv)mentioning

confidence: 99%

“…The left lung was tied, and the right lung was washed three times with 20 mL/kg cold sterile phosphate-buffered saline (PBS; pH 7.2-7.4). Lavage fluids were kept separate and centrifuged (at 400 x g for 10 min) at 4ºC, and the supernatants were frozen immediately on dry ice and stored at -80ºC 1 . …”

Section: Bronchoalveolar Lavage (Bal)mentioning

confidence: 99%

“…Hence, acid aspiration-content is an important cause of acute respiratory distress syndrome (ARDS) 1 .…”

Section: Introductionmentioning

confidence: 99%

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Effects of prone and supine position on oxygenation and inflammatory mediator in a hydrochloric acid-induced lung dysfunction in rats

et al. 2008

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Purpose:To compare the effectiveness of mechanical ventilation of supine versus prone position in hydrochloric acid (HCl)-induced lung dysfunction. Methods: Twenty, adult, male, Wistar-EPM-1 rats were anesthetized and randomly grouped (n=5 animals per group) as follows: CS-MV (mechanical ventilation in supine position); CP-MV (mechanical ventilation in prone position); bilateral instillation of HCl and mechanical ventilation in supine position (HCl+S); and bilateral instillation of HCl and mechanical ventilation in prone position (HCl+P). All groups were ventilated for 180 minutes. The blood partial pressures of oxygen and carbon dioxide were measured in the time points 0 (zero; 10 minutes before lung injury for stabilization), and at the end of times acid injury, 60, 120 and 180 minutes of mechanical ventilation. At the end of experiment the animals were euthanized, and bronchoalveolar lavages (BALs) were taken to determine the contents of total proteins, inflammatory mediators, and lungs wet-to-dry ratios. Results: In the HCl+P group the partial pressure of oxygen increased when compared with HCl+S (128.0±2.9 mmHg and 111.0±6.7 mmHg, respectively) within 60 minutes. TNF-α levels in BAL do not differ significantly in the HCl+P group (516.0±5.9 pg/mL), and the HCl+S (513.0±10.6 pg/mL). Conclusion:The use of prone position improved oxygenation, but did not reduce TNF-α in BAL upon lung dysfunction induced by HCl. Key words: Lung. Respiration, Artificial. Oxygenation. Inflammation Mediators. Rats. RESUMO Objetivo:Comparar os efeitos da ventilação mecânica em posição prona versus supina na disfunção pulmonar induzida por ácido clorídrico (HCl). Métodos: Vinte ratos, adultos, Wistar-EPM-1 foram anestesiados e distribuídos aleatoriamente em grupos (n=5 animais por grupo): CS-MV (controle, ventilado mecanicamente em posição supina); CP-MV (controle, ventilado mecanicamente em posição prona); instilação bilateral de HCl e ventilação mecânica em posição supina (HCl+S) ou ventilação em posição prona (HCl+P). Todos os grupos foram submetidos a ventilação mecânica por 180 minutos. As pressões parciais de oxigênio e dióxido de carbono no sangue arterial foram mensuradas nos tempos Injúria ácida (10 minutos após instilação de HCl), e ao final de cada período após lesão por HCl, 60, 120 e 180 minutos sob ventilação mecânica. Ao final do experimento os animais foram eutanasiados, os pulmões retirados para avaliação do peso úmido em relação ao peso seco do pulmão direito e realizamos o lavado broncoalveolar (BAL) para determinação de proteínas totais e o mediador inflamatório TNF-α. Resultados: No grupo HCl+P a pressão parcial de oxigênio, no tempo de 60 minutos, aumentou quando comparada com o grupo HCl+S (128.0±2.9 e 111.0±6.7 mmHg, respectivamente). Os níveis de TNF-α no lavado broncoalveolar não diferiram de maneira estatisticamente significante quando comparamos os grupos HCl+S (513.0±10.6 pg/mL) versus HCl+P (516.0±5.9 pg/mL). Conclusão: O uso da posição prona melhora a oxigenação, mas não reduz os níveis de BAL ...

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Section: Discussionmentioning

confidence: 99%

Section: Surgical Proceduresmentioning

confidence: 99%

“…A venous catheter was placed in the jugular vein to allow the infusion of isotonic saline solution and sodium pentobarbital whenever needed 1 .…”

Section: Mechanical Ventilation (Mv)mentioning

confidence: 99%

Section: Bronchoalveolar Lavage (Bal)mentioning

confidence: 99%

“…Hence, acid aspiration-content is an important cause of acute respiratory distress syndrome (ARDS) 1 .…”

Section: Introductionmentioning

confidence: 99%

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Effects of prone and supine position on oxygenation and inflammatory mediator in a hydrochloric acid-induced lung dysfunction in rats

et al. 2008

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Pentoxifylline alleviates vascular impairment in insulin resistance via TNF-α inhibition

El-Bassossy

El‐Moselhy

Mahmoud

2011

Naunyn-Schmiedeberg's Arch Pharmacol

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Deterioration of vascular reactivity plays a pivotal role in vascular complications. Pentoxifylline (PTX) is a well-tolerated drug used to treat vascular insufficiency. We investigated the protective effect of PTX against vascular impairment in insulin resistance. Insulin resistance was induced by fructose (10%) in drinking water while PTX was concurrently administered (50 mg/kg(-1)) for 8 weeks. Serum levels of glucose, insulin, tumor necrosis factor alpha (TNF-α) were determined. Isolated aorta reactivity to phenylephrine (PE), potassium chloride (KCl), and acetylcholine (ACh) was studied, as was nitric oxide (NO) generation and histopathology. Insulin resistance was accompanied with a significant elevation in serum TNF-α level, marked leukocytes infiltration, and endothelial pyknosis. PTX inhibited insulin resistance and prevented TNF-α elevation, leukocyte infiltration and endothelial pyknosis. Vascular dysfunction was evident in insulin resistance as increased vascular contractility to PE and decreased relaxation to ACh, whereas PTX protected against this dysfunction. Notably, in vitro incubation with TNF-α (1 ng/ml(-1)) increased contractility to PE and decreased relaxation to ACh while concomitant PTX (1 mM) incubation partially restored response to ACh but not to PE. Furthermore, TNF-α reduced ACh-induced NO generation, whereeas PTX restored it. In conclusion, PTX protects from the impairment in vascular reactivity in insulin resistance, by a mechanism involving TNF-α inhibition.

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TNF-α and Pulmonary Diseases

Mukherjee¹,

Malik²,

Sanders³

et al. 2022

Encyclopedia of Respiratory Medicine

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Effects of Pentoxifylline on TNF-Alpha and Lung Histopathology in HCl-Induced Lung Injury

Cited by 15 publications

References 34 publications

Effects of prone and supine position on oxygenation and inflammatory mediator in a hydrochloric acid-induced lung dysfunction in rats

Effects of prone and supine position on oxygenation and inflammatory mediator in a hydrochloric acid-induced lung dysfunction in rats

Pentoxifylline alleviates vascular impairment in insulin resistance via TNF-α inhibition

TNF-α and Pulmonary Diseases

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