Characterization of an Animal Model of Severe Sepsis Associated With Respiratory Dysfunction

Azevedo, Luciano César Pontes; Park, Marcelo; Noritomi, Danilo Teixeira; Maciel, Alexandre Toledo; Brunialti, Milena Karina Coló; Salomão, Reinaldo

doi:10.1590/s1807-59322007000400017

Cited by 26 publications

(17 citation statements)

References 20 publications

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“…However, we found that a relatively large number of such articles were published in the two journals. The journal Clinics contained a large number of ex-perimental studies (n = 14), mostly using rat models, but also one study using a pig model (analyzing respiratory dysfunction in relation to sepsis) 23 and three experimental studies on the knees of human cadavers. [24][25][26] For both journals, the most common clinical questions addressed in the articles related to the prevalence, frequency or associations between variables, which were answered using cross-sectional studies.…”

Section: Discussionmentioning

confidence: 99%

Design and level of evidence of studies published in two Brazilian medical journals recently indexed in the ISI Web of Science database

Torloni

Riera

2010

Sao Paulo Med. J.

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CONTEXT AND OBJECTIVES:The level of evidence and methodological quality of articles published in medical journals are important aids for clinicians in decision-making and also affect journals' impact factor. Although systematic reviews (SR) are considered to represent the highest level of evidence, their methodological quality is not homogeneous and they need to be as carefully assessed as other types of study. This study aimed to assess the design and level of evidence of articles published in 2007, in two recently indexed Brazilian journals (Clinics and Revista da Associação Médica Brasileira), and to evaluate the methodological quality of the SRs. DESIGN AND SETTING:Descriptive study developed in the Brazilian Cochrane Center, Universidade Federal de São Paulo.METHODS: All 289 published articles were classified according to types of study design and level of evidence. The SRs were critically appraised by two evaluators using the AMSTAR tool. RESULTS:The most frequent design types were cross-sectional studies (39.9%), case reports (15.8%), experimental studies (10.8%) and narrative reviews (7.4%). According to the Oxford criteria, 25.6% of the articles were classified as level 4 or 5 evidence, while 2.8% were level 1. SRs represented only 2% of the published articles and their methodological quality scores were low. CONCLUSIONS:The main design types among the published papers were observational and experimental studies and narrative reviews. SRs accounted for a small proportion of the articles and had low methodological scores. Brazilian medical journals need to encourage publication of greater numbers of clinically relevant papers of high methodological quality.RESUMO CONTEXTO E OBJETIVOS: O nível de evidência e a qualidade metodológica dos artigos publicados em revistas médicas são importantes para auxiliar os clínicos a tomarem decisões e também afetam o fator de impacto dos periódicos. Apesar de as revisões sistemáticas (RS) serem consideradas o maior nível de evidência, sua qualidade metodológica não é homogênea e elas precisam ser avaliadas tão criteriosamente como outros tipos de estudos. O objetivo deste estudo foi avaliar os desenhos e nível de evidência dos artigos publicados em 2007 em duas revistas brasileiras recentemente indexadas (Clinics e Revista da Associação Médica Brasileira) e avaliar a qualidade metodológica das RS. TIPO DE ESTUDO E LOCAL:Estudo descritivo desenvolvido no Centro Cochrane do Brasil, Universidade Federal de São Paulo. MÉTODOS:Todos os 289 artigos publicados foram classificados quanto ao tipo de desenho e nível de evidência. As RS foram analisadas criticamente por dois revisores usando o instrumento AMSTAR.RESULTADOS: Os tipos de desenhos mais frequentes foram os transversais (39,9%), relatos de casos (15,8%), estudos experimentais (10,8%) e revisões narrativas (7,4%). Segundo os critérios de Oxford, 25,6% dos artigos eram de nível 4 ou 5 e 2,8% de nível 1. As RS representaram apenas 2% do total dos artigos publicados e tiveram notas de qualidade metodológica baixas...

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Section: Discussionmentioning

confidence: 99%

Design and level of evidence of studies published in two Brazilian medical journals recently indexed in the ISI Web of Science database

Torloni

Riera

2010

Sao Paulo Med. J.

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“…Over a decade of studies in cells, mice, large animals, and humans, we have developed an integrated view of the postinjury acute inflammatory response, which is regulated by initial chemokine-driven cues and propagated by DAMPs. These studies involved multiple paradigms of acute inflammation, including endotoxemia and experimental sepsis in mice (32,35,109,125), rats (39,107,112), and swine (43,50,112,116)), experimental trauma/ hemorrhagic shock in mice (35,45,81,84,97,125), and traumatic injury in humans (3,31,84,110,136,158).…”

Section: The Conundrum Of Acute Inflammationmentioning

confidence: 99%

Insights into the Role of Chemokines, Damage-Associated Molecular Patterns, and Lymphocyte-Derived Mediators from Computational Models of Trauma-Induced Inflammation

Namas

et al. 2015

Antioxidants & Redox Signaling

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Significance: Traumatic injury elicits a complex, dynamic, multidimensional inflammatory response that is intertwined with complications such as multiple organ dysfunction and nosocomial infection. The complex interplay between inflammation and physiology in critical illness remains a challenge for translational research, including the extrapolation to human disease from animal models. Recent Advances: Over the past decade, we and others have attempted to decipher the biocomplexity of inflammation in these settings of acute illness, using computational models to improve clinical translation. In silico modeling has been suggested as a computationally based framework for integrating data derived from basic biology experiments as well as preclinical and clinical studies. Critical Issues: Extensive studies in cells, mice, and human blunt trauma patients have led us to suggest (i) that while an adequate level of inflammation is required for healing post-trauma, inflammation can be harmful when it becomes self-sustaining via a damage-associated molecular pattern/Toll-like receptor-driven feed-forward circuit; (ii) that chemokines play a central regulatory role in driving either self-resolving or selfmaintaining inflammation that drives the early activation of both classical innate and more recently recognized lymphoid pathways; and (iii) the presence of multiple thresholds and feedback loops, which could significantly affect the propagation of inflammation across multiple body compartments. Future Directions: These insights from data-driven models into the primary drivers and interconnected networks of inflammation have been used to generate mechanistic computational models. Together, these models may be used to gain basic insights as well as serving to help define novel biomarkers and therapeutic targets. Antioxid. Redox Signal. 23, 1370-1387.Trauma: A Significant Burden T rauma/hemorrhagic shock remains the leading cause of death in patients younger than 45 years (70). It is the third leading cause of death worldwide, resulting in five million or 10% of all deaths annually and thus considered the fifth leading cause of significant disability (137). Traumatic injury is a pandemic disease, one that affects every nation in the world regardless of the level of socioeconomic development (70, 71).The disease is acute in onset, but often results in chronic, debilitating health problems that have effects beyond the individual victims. The financial impact of traumatic injuries is staggering: in 2000 in the United States, 10% of hospital discharges were due to injuries, and the direct cost of treating 50 million injury cases was $80.2 billion, with an estimated

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“…Additionally, LPS was shown to cause a strong inflammatory response that leads to endocardium cells necrosis (de Azevedo et al 2007).…”

Section: Discussionmentioning

confidence: 99%

The influence of ETA and ETB receptor blockers on LPS-induced oxidative stress and NF-κB signaling pathway in heart

Piechota-Polańczyk¹,

Kleniewska²,

Gorąca³

2012

gpb

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Abstract. The aim of this study was to assess whether an endothelin-A receptor (ETA-R) blocker, BQ123, or an endothelin-B (ETB-R) receptor blocker, BQ788, influences nuclear factor kappa beta (NF-κB) pathway, free radical generation, tumor necrosis factor-alpha (TNF-α) concentration, and glutathione redox system in hearts obtained from lipopolysaccharide (LPS)-induced endotoxic rats. The study was performed on rats divided into groups: 1) saline, 2) saline + LPS (15 mg/kg), 3) BQ123 (1 mg/kg b.w.) + LPS, 4) BQ123 (0.5 mg/kg b.w.) + LPS, 5) BQ788 (3 mg/kg b.w.) + LPS. The ETA-R and ETB-R antagonists were injected i.v. 30 min before LPS administration. In rats, BQ123 caused a significant decrease in TBARS (p < 0.05) but not in H 2 O 2 concentration. It also decreased tissue protein level and improved tissue redox status (p < 0.01). Only a dose of 1 mg/kg decreased TNF-α concentration (p < 0.05). BQ788 lowered TBARS, H 2 O 2 and protein concentration (p < 0.05; p < 0.02; p < 0.001, respectively), however, it did not affect TNF-α concentration. Neither ETA-R nor ETB-R blockers influenced LPS-induced increase in p65 subunit level and activation of NF-κB pathway. Our results demonstrated that ETA-R blockage is more effective in inhibiting free radical generation and improving heart antioxidant properties than ETB-R blockage under oxidative stress. NF-κB pathway is not incorporated in ETA-R and ETB-R influence on ROS production.

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Characterization of an Animal Model of Severe Sepsis Associated With Respiratory Dysfunction

Cited by 26 publications

References 20 publications

Design and level of evidence of studies published in two Brazilian medical journals recently indexed in the ISI Web of Science database

Design and level of evidence of studies published in two Brazilian medical journals recently indexed in the ISI Web of Science database

Insights into the Role of Chemokines, Damage-Associated Molecular Patterns, and Lymphocyte-Derived Mediators from Computational Models of Trauma-Induced Inflammation

The influence of ETA and ETB receptor blockers on LPS-induced oxidative stress and NF-κB signaling pathway in heart

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