Oxidative Stress Is Enhanced by Hypothermia Imposed on Cerulein-Induced Pancreatitis in Rats

Andraus, Wellington; Jukemura, José; Dutra, Fernando; Bechara, Etelvino J.H.; Cunha, José Eduardo Monteiro da; Machado, Marcel Cerqueira César

doi:10.1590/s1807-59322007000400016

Cited by 14 publications

(8 citation statements)

References 41 publications

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“…Окислительный стресс приводит к увеличению уровня супероксидных радикалов, в том числе активных форм кислорода [34]. Взаимодействие между супероксидными радикалами и NO играет одну из ключевых ролей в развитии панкреатита [2], в результате чего развивается дефицит биологически активного внутриклеточного NO [26]. В предыдущих исследованиях мы показали, что окислительный стресс предшествует активации NOS при развитии воспаления поджелудочной железы [6,7].…”

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Oxidative Stress and NO Generation in Cerulein-Induced Acute Pancreatitis in Rats

Тиманн

Шнекенбургер²,

Шик³

et al. 2019

Žurnal anatomii i gistopatologii

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2 Центр биомедицинских технологий Университетской клиники Мюнстера, г. Мюнстер, Германия 3 Биоэкологический центр, г. Галле, Германия 4 Университетская клиника Шарите, г. Берлин, Германия 6 ФГБОУ ВО «Воронежский государственный медицинский университет им. Н.Н. Бурденко» Минздрава России, г. Воронеж, РоссияВзаимодействие оксида азота (NO) и супероксидных радикалов является важным механизмом развития панкреатита. В предыдущих исследованиях мы показали роль окислительного стресса и NO синтазы (NOS) в развитии хронического панкреатита человека на модели у крыс с перевязкой протока поджелудочной железы. Выявлено, что развитие окислительного стресса предшествует активации NOS. Это позволяет интерпретировать усиление продукции NO в ходе панкреатита в качестве адаптационного механизма, направленного на поддержание определенного уровня биологически активного NO в клетке. В статье представлены данные о состоянии NOS и маркеров окислительного стресса (нитротирозина и 8-гидроксигуанозина) при остром панкреатите у крыс, вызванном церулеином. Нами показано, что особенности взаимодействия супероксидных радикалов/NO в данной модели острого панкреатита аналогичным тем, которые наблюдаются при перевязке панкреатического протока, а также при хроническим панкреатите у человека. Таким образом, индуцированный церулеином пакреатит аналогичен последствиям воспаления поджелудочной железы при перевязке ее протока и является удобной моделью для дальнейших исследований патогенеза данного заболевания у человека.Ключевые слова: острый панкреатит, оксид азота, NO синтаза, оксидативный стресс.The interaction between nitric oxide (NO) and superoxides is critical in the development of pancreatitis. Previously, we reported on the up-regulation of oxidative stress and NO-synthase (NOS) in the human chronic pancreatitis and in an animal model of pancreatitis induced by pancreatic duct ligation (PDL) in rats. We have shown that oxidative stress runs ahead of NOS up-regulation, which implies that the NO enhancement in the course of pancreatitis is likely to be an adaptive mechanism aimed at maintaining the homeostatic cellular level of the bioactive NO. Here, we report on the expression of NOS and oxidative stress markers (nitrotyrosine and 8-hydroxyguanosine) in the course of cerulein-induced acute pancreatitis in rats. We found that the pattern of superoxides/NO interaction in this model of acute pancreatitis is similar to that in the PDL-induced rat pancreatitis and in the human chronic pancreatitis. It means that cerulein-induced acute pancreatitis like the PDL-induced pancreatitis is a proper model for further studies of pancreatitis development in humans.

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Oxidative Stress and NO Generation in Cerulein-Induced Acute Pancreatitis in Rats

Тиманн

Шнекенбургер²,

Шик³

et al. 2019

Žurnal anatomii i gistopatologii

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“…Reactive oxygen species (ROS), such as superoxide anions (SO 2 ‐ ) and hydrogen peroxide (H 2 O 2 ), once thought only to be harmful byproducts of oxidative metabolism, are now recognized as critical second messengers in a wide range of physiological processes 1–4. ROS are the result of incomplete reduction of oxygen to O 2 − , which is spontaneously or enzymatically dismutated to H 2 O 2 by superoxide dismutase (SOD).…”

Section: Introductionmentioning

confidence: 99%

ATZ (3-amino-1,2,4-triazole) injected into the fourth cerebral ventricle influences the Bezold–Jarisch reflex in conscious rats

Valenti

Abreu

Sato

et al. 2010

Clinics

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OBJECTIVES:Many studies have investigated the importance of oxidative stress on the cardiovascular system. In this study we evaluated the effects of central catalase inhibition on cardiopulmonary reflex in conscious Wistar rats.METHODS:Male Wistar rats were implanted with a stainless steel guide cannula in the fourth cerebral ventricle. The femoral artery and vein were cannulated for mean arterial pressure and heart rate measurement and for drug infusion, respectively. After basal mean arterial pressure and heart rate recordings, the cardiopulmonary reflex was tested with a dose of phenylbiguanide (PBG, 8 µg/kg, bolus). Cardiopulmonary reflex was evaluated before and µl15 minutes after 1.0 µL 3‐amino‐1,2,4‐triazole (ATZ, 0.01g/100µL)0.01 g/100 µL) injection into the fourth cerebral ventricle. Vehicle treatment did not change cardiopulmonary reflex responses.RESULTS:Central ATZ significantly increased hypotensive responses without influencing the bradycardic reflex.CONCLUSION:ATZ injected into the fourth cerebral ventricle increases sympathetic inhibition but does not change the parasympathetic component of the cardiopulmonary reflex in conscious Wistar rats.

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“…More than 20 years ago, Selye1 recognized that the physiological system is activated by stress and that protecting and restoring the body can also damage the system 2,3. Exposure to low temperatures is considered to be an important stressing physical agent 4,5.…”

Section: Introductionmentioning

confidence: 99%

Electric Countershock and Cold Stress Effects on Liver and Adrenal Gland

Ferreira

Abreu

Valenti

et al. 2010

Clinics

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OBJECTIVES:Cold exposure induces glycogen and lipid depletion in the liver and the adrenal gland, respectively. However, no previous study has determined the effects of electrical countershock on those tissues. We aimed to evaluate the effects of electrical countershock on lipid depletion in the adrenal gland and on glycogen depletion in the liver.METHODS:We used 40 male Wistar rats divided into four groups: the control group, in which the animals were subjected to a resting period of seven days; the electrical discharge group, in which the animals were subjected to a resting period followed by administration of ten 300-mV electrical discharges; the electrical post-discharge group, in which the animals received ten electrical shocks (300 mV) followed by rest for seven consecutive days; and the cold stress group, in which the animals were subjected to a resting period and were then exposed to −8ºC temperatures for four hours. All animals underwent a laparotomy after treatment. The lipid and glycogen depletions are presented using intensity levels (where + = low intensity and ++++ = high intensity, with intermediate levels in between).RESULTS:The rats exposed to the cold stress presented the highest glycogen and lipid depletion in the liver and the adrenal gland, respectively. Furthermore, we noted that the electrical countershock significantly increased lipid depletion in the adrenal gland and glycogen depletion in the liver. One week after the electrical countershock, the liver and adrenal gland profiles were similar to that of the control group.CONCLUSION:Electrical countershock immediately increased the glycogen depletion in the liver and the lipid depletion in the adrenal gland of rats.

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Oxidative Stress Is Enhanced by Hypothermia Imposed on Cerulein-Induced Pancreatitis in Rats

Cited by 14 publications

References 41 publications

Oxidative Stress and NO Generation in Cerulein-Induced Acute Pancreatitis in Rats

Oxidative Stress and NO Generation in Cerulein-Induced Acute Pancreatitis in Rats

ATZ (3-amino-1,2,4-triazole) injected into the fourth cerebral ventricle influences the Bezold–Jarisch reflex in conscious rats

Electric Countershock and Cold Stress Effects on Liver and Adrenal Gland

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