Diacerhein Versus Glucosamine in a Rat Model of Osteoarthritis

Rezende, Márcia Uchôa de; Gurgel, Henrique Melo de Campos; Vilaça, Paulo Roberto; Kuroba, Rogério Kawassaki; Lopes, Alex Silva Santiago; Phillipi, Renée Zon; Hernandez, Arnaldo José

doi:10.1590/s1807-59322006000500014

Cited by 32 publications

(47 citation statements)

References 14 publications

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“…OA-operated knees were dissected from the coxofemoral region to the ankle region, leaving the articular capsule intact. Following dissection, the maximum extension angle of each knee was measured as described previously (19), with 0˚ corresponding to the maximum possible extension. To minimize bias, all surgeries and measurements of the extension level were performed by the same veterinarian.…”

Section: Effect Of Polycalcium a Mixture Of Polycan And Calcium Lactmentioning

confidence: 99%

Effect of Polycalcium, a mixture of Polycan and calcium lactate-gluconate in a 1:9 weight ratio, on rats with surgery-induced osteoarthritis

Choi¹,

Shin²,

Kim³

et al. 2015

Experimental and Therapeutic Medicine

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Abstract. In the present study, the beneficial and synergistic effects of Polycalcium, a mixture of Polycan and calcium (Ca) lactate-gluconate in a 1:9 weight ratio, on a rat model of osteoarthritis (OA) were explored. Polycalcium (50, 100 and 200 mg/kg) was administered orally once per day for 28 days from 1 week after the OA-modeling surgery. Diclofenac sodium (2 mg/kg) was administered as a reference drug. Following the OA surgery, increases in the maximum extension angles, edematous changes in knee and capsule thickness, reductions in chondrocyte proliferation and cartilage glycosaminoglycan (GAG) levels, as well as changes in cartilage degeneration were observed. However, these OA-related symptoms were inhibited after 28 days of continuous oral treatment with Polycalcium. Anti-OA effects, including the induction of chondrocyte proliferation, were detected in the Polycalcium-treated rats and were more favorable compared with those in rats treated with Polycan or Ca lactate-gluconate alone (100 mg). Therefore, a mixture of Polycan and Ca lactate-gluconate was demonstrated to have beneficial synergistic effects on OA.

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Section: Effect Of Polycalcium a Mixture Of Polycan And Calcium Lactmentioning

confidence: 99%

Effect of Polycalcium, a mixture of Polycan and calcium lactate-gluconate in a 1:9 weight ratio, on rats with surgery-induced osteoarthritis

Choi¹,

Shin²,

Kim³

et al. 2015

Experimental and Therapeutic Medicine

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“…Table 3 and in vivo to reduce all cartilage degeneration parameters and joint stiffness. 6,30 It modulates the expression of matrix-degrading enzymes and cell proliferation of articular chondrocytes through inhibiting ERK and JNK-AP-1 dependent pathways. 31 It downregulates pro-inflammatory cytokine expression (interleukin-1-beta (IL-1b), IL-12 and TNF-alpha).…”

Section: Discussionmentioning

confidence: 99%

Experimental osteoarthritis model by means of medial meniscectomy in rats and effects of diacerein administration and hyaluronic acid injection

et al. 2014

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CONTEXT AND OBJECTIVE: The development of a slow and progressive mechanical model for osteoarthritis is important for correlation with clinical practice, and for evaluating the effects of disease-modifying medications. A mechanical osteoarthritis model was developed to evaluate the effects of intra-articular hyaluronic acid (HA) injection and oral diacerein administration. DESIGN AND SETTING: Experimental study at the Department of Orthopedics and Traumatology, Universidade de São Paulo. METHOD: Total medial meniscectomy was performed on seven groups of ten Wistar rats each, comprising four control groups (C) and three study groups (S). C.I: operated, non-medicated; C.II: operated, injections of HA vehicle; C.III: non-operated, non-medicated; C.IV: operated, non-medicated, sacrificed three months post-meniscectomy; S.I: operated, receiving intra-articular HA injections; S.II: operated, oral diacerein from the third to the seventh postoperative month; S.III: operated, received both medications. All the animals (except C.IV) were sacrificed seven months post-meniscectomy. All femurs and tibias were assessed histologically. RESULTS: The most severe degenerative histological changes were in the tibias of the operated knees. On the contralateral side, all groups had mild changes on the tibial surface. The femoral surface had slight changes. C.I showed severe changes. S.II results matched those of C.IV. HA protected the tibial surface. S.II and S.III had similar results. CONCLUSIONS: 1) The experimental model produced mild arthritis after three months and severe arthritis after seven months; 2) diacerein reduced the degenerative changes in both knees; 3) HA protected the joint cartilage; 4) Combining the two drugs did not improve the results.RESUMO CONTEXTO E OBJETIVO: Desenvolver um modelo osteoartrítico mecânico lento e progressivo é importante para correlação com a prática clínica e para avaliar os efeitos de medicamentos modificadores da doença. Um modelo mecânico de osteartrite foi desenvolvido para avaliar os efeitos de injeção intra-articular de hialuronato de sódio (AH) e de administração de diacereína oral. DESENHO E LOCAL: Estudo experimental no Departamento de Ortopedia e Traumatologia, Universidade de São Paulo. MÉTODO: Meniscectomia medial total foi feita em sete grupos de dez ratos Wistar, sendo quatro grupos controle (C) e três grupos estudo (E). C.I: operado, não medicado; C.II: operado, recebendo injeções do veículo do AH; C.III: não operado, não medicado; C.IV: operado, não medicado, sacrificado três meses pós-meniscectomia; EI: operado, recebendo injeções de AH intra-articular; E.II: operado, recebendo diacereína oral do terceiro ao sétimo mês pós-operatório; E.III: operado, recebeu ambas medicações. Todos os animais (exceto C.IV) foram sacrificados sete meses pós-meniscectomia. Todos os fêmures e tíbias foram analisados histologicamente. RESULTADOS: As alterações histológicas degenerativas mais graves encontram-se nas tíbias dos joelhos operados. No lado contra-lateral, todos os grupos apre...

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“…Dose selection was based on preliminary studies. [1,4] Rats were anesthetized with ketamine (0.25 mg/100 g) and xylazine (10 mg/100 g) IP. Once the animal was sedated, the skin overlying knee was shaved.…”

Section: Methodsmentioning

confidence: 99%