Radiodensitometric and DXA analyses for the measurement of bone mineral density after systemic alendronate therapy

Abstract

Precise techniques for the measurement of maxillary bone mineral density (BMD) are useful for the early diagnosis of systemic diseases. The aim of this study was to compare in vivo the efficacy of dualenergy x-ray absorptiometry (DXA) and radiographic densitometry for the measurement of BMD after systemic administration of sodium alendronate. Wistar rats were randomly allocated to a control group (n = 5), which received distilled water, and a sodium alendronate group (n = 8), which received two doses of chemically pure sodium alendronate (1 mg/ kg) per week. After 8 weeks, the animals were euthanized, the tibias were removed, and the BMD of the proximal tibial metaphysis was analyzed radiographically and by DXA. The data were subjected to statistical analysis by the Kruskal-Wallis test at a significance level of 5%. Both of the techniques revealed that the alendronate-treated group had a significantly higher BMD (p < 0.05) than the control group after 8 weeks of treatment. Comparing the groups with and without alendronate therapy revealed increases of 14.9% and 29.6% in BMD, as detected radiographically and by DXA, respectively. In conclusion, both of the methods were able to detect an increase in BMD of the proximal tibial metaphysis after alendronate therapy.

Efecto de Vitamina E y Alendronato sobre la Densidad Mineral en Hueso Maxilar de Ratas

“…Por otro lado, Lucisano et al (2013) analizaron la DMO en ratas, después de la administración sistémica de AL, utilizando dosis de 1 mg/kg por semana. En su trabajo demostraron una DMO significativamente mayor que el grupo control recién después de 8 semanas de tratamiento (60 días).…”

Intermittent administration of parathyroid hormone improves the repairing process of rat calvaria defects: A histomorphometric and radiodensitometric study

“…We also investigated the bone mineral density (BMD) that is widely used to evaluate alterations in the balance between osteoblastic bone formation and osteoclastic bone resorption ( 25 ). The measurement of BMD through radiographic densitometry has been performed in a number of studies, and its efficacy has been confirmed ( 25 - 27 ).…”

“…We also investigated the bone mineral density (BMD) that is widely used to evaluate alterations in the balance between osteoblastic bone formation and osteoclastic bone resorption ( 25 ). The measurement of BMD through radiographic densitometry has been performed in a number of studies, and its efficacy has been confirmed ( 25 - 27 ). Radiographic densitometry analysis is an important tool for the research evaluation because it is a rapid and low-cost method, and can produce results which are similar to those of dual-energy x-ray absorptiometry ( 25 ).…”

Alendronate inhibits osteocyte apoptosis and inflammation via IL‐6, inhibiting bone resorption in periapical lesions of ovariectomized rats

“…The data were recorded and analysed by the Lunar PIXImus software, version 2.2 (Lunar PIXImus Corp. Headquarters), as described previously (Lucisano et al . ).…”

“…One femur from each animal of all groups was surgically removed and analysed by Lunar PIXImus dual-energy X-ray absorptiometry densitometry (Lunar PIXImus Corp. Headquarters, Madison, WI, USA) for recording the BMD of the distal metaphysis, in g cm À2 . The data were recorded and analysed by the Lunar PIXImus software, version 2.2 (Lunar PIXImus Corp. Headquarters), as described previously (Lucisano et al 2013).…”