In vivo qualitative analysis of the biocompatibility of different cyanoacrylate-based adhesives

Abstract

Cyanocrylates have been widely used in the medical and dental fields for several years. In Dentistry, cyanoacrylates have been used for suturing, pulp capping, as retrofilling material in endodontic surgeries, and as cervical plug for pulpless teeth bleaching. The biocompatibility of these adhesives has been the topic of many researches and subcutaneous implantation is an effective methodology for these studies. The present study evaluated the biocompatibility of three different cyanoacrylate-based adhesives. Thirty-six Wistar rats were used, divided into four groups of 9 animals each: A (control) -distilled water, B -cyanoacrylate ester (Super Bonder), C -n-butyl-cyanoacrylate (Histoacryl) and D -alpha-cyanoacrylate (Three Bond). The materials were dispensed in sponges of polyvinyl chloride, the animals were incised and the sponges were inserted in the subcutaneous tissue and sutured. Each group was sub-divided according to the time of sacrifice of the animals: 7, 21 and 45 days. Subjective analysis of the histologic material showed that all groups presented some degree of irritability, but the inflammatory reaction decreased with the experimental time in all groups. Group D showed an inflammatory reaction which was closer to that of the control group and was considered to have good biocompatibility. Groups B and C were similar and presented more aggressive inflammatory reactions when compared to the control group. Based on the results, it was concluded that alpha-cyanoacrylate (Three Bond) was the most biocompatible adhesive because it caused the lowest levels of inflammation.

Biocompatibility assessment of pastes containing Copaiba oilresin, propolis, and calcium hydroxide in the subcutaneous tissue of rats

“…From a biological standpoint, their biocompatibility must be evaluated, because the eventual toxic components present may cause tissue irritation, degeneration or necrosis of the tissues adjacent to the materials. [2223]…”

“…After sectioning, each tube had one of the extremities sealed with Cyanoacrylate ester gel (Super Bonder, Aachen, Germany ) to avoid extravasation of the material to be tested. [22]…”

Elasticity and safety of alkoxyethyl cyanoacrylate tissue adhesives

“…Local swelling at the surgical site was measured with calipers, then the tissue and skin surrounding the glues were harvested and processed for hematoxylin and eosin staining by standard techniques [26]. …”

Biocompatibility Evaluation of Epiphany/Resilon Root Canal Filling System in Subcutaneous Tissue of Rats

“…The sealers in the tubes of ERSP and ER groups were photoactivated according to manufacturer's recommendation. After this, one of the extremities of each tube was sealed with cyanoacrylate ester gel (Super Bonder, Aachen, Germany) to avoid extravasation of material to be tested (19) and immediately implanted in the subcutaneous tissue of the rats.…”