Localization of Bmp-4, Shh and Wnt-5a transcripts during early mice tooth development by in situ hybridization

Nunes, Fábio Daumas; Valenzuela, Maria da Graça Silva; Rodini, Camila Oliveira; Massironi, Sílvia Maria Gomes; Ko, Gui Mi

doi:10.1590/s1806-83242007000200006

Cited by 6 publications

(5 citation statements)

References 25 publications

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“…During tooth crown formation, the cells in the inner enamel epithelium differentiate into ameloblasts that form the enamel. The inner enamel epithelium at this stage expresses both Shh and Ptch [24,[49][50][51][52][53][54], and suppression of these expressions results in the inhibition of the proliferative activity of the epithelial cells [35]. In addition, it has been demonstrated that inhibition of Shh signaling in tooth germ using a neutralizing antibody suppresses ameloblast differentiation [36].…”

Section: Shh Regulates Enamel Formationmentioning

confidence: 99%

Sonic Hedgehog Signaling and Tooth Development

Hosoya

Shalehin

Takebe

et al. 2020

IJMS

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Sonic hedgehog (Shh) is a secreted protein with important roles in mammalian embryogenesis. During tooth development, Shh is primarily expressed in the dental epithelium, from initiation to the root formation stages. A number of studies have analyzed the function of Shh signaling at different stages of tooth development and have revealed that Shh signaling regulates the formation of various tooth components, including enamel, dentin, cementum, and other soft tissues. In addition, dental mesenchymal cells positive for Gli1, a downstream transcription factor of Shh signaling, have been found to have stem cell properties, including multipotency and the ability to self-renew. Indeed, Gli1-positive cells in mature teeth appear to contribute to the regeneration of dental pulp and periodontal tissues. In this review, we provide an overview of recent advances related to the role of Shh signaling in tooth development, as well as the contribution of this pathway to tooth homeostasis and regeneration.

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Section: Shh Regulates Enamel Formationmentioning

confidence: 99%

Sonic Hedgehog Signaling and Tooth Development

Hosoya

Shalehin

Takebe

et al. 2020

IJMS

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“…In tooth germ mesenchyme, gene expressions of Wnt-5a and Wnt-10a have been reported 4), 5), 16) . For Wnt-10a in particular it has been reported that the expression was observed in enamel knot in early tooth germ, then shifted to the underlying mesenchymal cells, and was observed in preodontoblasts and odontoblasts at a later stage 5) .…”

Section: Wnt/ß-catenin Signaling Pathways In Odontoblasts and Progenimentioning

confidence: 99%

Immunolocalization of the Factors Related to Wnt Signaling Pathway in Developing Rat Molar

Moriguchi

Yamada

Miake

et al. 2011

J. Hard Tissue Biology.

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In the Wnt/β-catenin signaling pathway, Wnt signal is transmitted to glycogen synthase kinase-3β (GSK-3β) through Dishevelled (Dvl), GSK-3β activity is inhibited, β-catenin phosphorylation is inhibited by the inactive-type GSK-3β, and β-catenin is transferred to the nucleus where it interacts with lymphoid enhancing factor (LEF)/T-cell factor (TCF), a transcription factor, which is considered to induce and regulate gene expression. In tooth development, it has been reported that Wnt and LEF are expressed at the earliest stage and are related to tooth development, but there are few reports on the situation at a later stage, and there have been no reports on Dvl and GSK-3β. In this study, we immunohistochemically examined the distribution of factors related to the Wnt/β-catenin signaling pathway, Wnt10, Dvl, GSK-3β, p-GSK-3β (inactive GSK-3β), and β-catenin, using serial sections of rat first molar germ to investigate the role of the Wnt signaling pathway in tooth germ development and tooth morphogenesis. Immunostaining for anti-Wnt10, anti-Dvl, anti-GSK-3β, anti-p-GSK-3β, and anti-β-catenin showed positive reactions at the inner enamel epithelium of tooth germ and weakly positive reactions at the dental papilla cells in contact with the inner enamel epithelium at embryonic day 19. At 8 days after birth, immunostaining for every antibody showed positive reactions for preameloblasts and preodontoblasts and more clearly positive reactions for secretory ameloblasts and odontoblasts. These results suggest that Wnt10, Dvl, GSK-3β, p-GSK-3β, and β-catenin are distributed in inner enamel epithelium, secretory ameloblasts, and odontoblasts, and that the Wnt/β-catenin signaling pathway via Dvl and p-GSK-3β is involved in these cells. In addition, for each of the factors, differentiated secretory cells showed more clearly positive reactions than undifferentiated cells; therefore, we conclude that the Wnt10 signaling pathway may be involved in differentiation to ameloblasts and odontoblasts, as well as secretory functions of ameloblasts and odontoblasts.

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“…In situ hybridization (ISH) was performed as previously described (Wu and Oh 1996) with the sonic hedgehog (Shh) gene being used as a positive control (Nunes et al 2007) and the sense riboprobe being used as a negative control. Color development of the hybridization signal was performed between 2-4 h.…”

Section: T T C G a A A A At G Ata T T T C C A A A G T T T G C A G G Amentioning

confidence: 99%

Development of secondary palate requires strict regulation of ECM remodeling: sequential distribution of RECK, MMP-2, MMP-3, and MMP-9

et al. 2010

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We have evaluated RECK (reversion-inducing-cysteine-rich protein with Kazal motifs), MMP-2 (matrix metalloproteinase-2), MMP-3, and MMP-9 involvement during palate development in mice by using various techniques. Immunohistochemical features revealed the distribution of RECK, MMP-2, and MMP-3 in the mesenchymal tissue and in the midline epithelial seam at embryonic day 13 (E13), MMPs-2, -3, and -9 being particularly expressed at E14 and E14.5. In contrast, RECK was weakly immunostained at these times. Involvement of MMPs was validated by measuring not only their protein expression, but also their activity (zymograms). In situ hybridization signal (ISH) for RECK transcript was distributed in mesenchymal and epithelial regions within palatal shelves at all periods evaluated. Importantly, the results from ISH analysis were in accord with those obtained by real-time polymerase chain reaction. The expression of RECK was found to be temporally regulated, which suggested possible roles in palatal ontogeny. Taken together, our results clearly show that remodeling of the extracellular matrix is finely modulated during secondary palate development and occurs in a sequential manner.

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Localization of Bmp-4, Shh and Wnt-5a transcripts during early mice tooth development by in situ hybridization

Cited by 6 publications

References 25 publications

Sonic Hedgehog Signaling and Tooth Development

Sonic Hedgehog Signaling and Tooth Development

Immunolocalization of the Factors Related to Wnt Signaling Pathway in Developing Rat Molar

Development of secondary palate requires strict regulation of ECM remodeling: sequential distribution of RECK, MMP-2, MMP-3, and MMP-9

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