Epstein-Barr virus in oral hairy leukoplakia scrapes: identification by PCR

Komatsu, Tatiana Lucianelli; Rivero, Elena Riet Corrêa; Magalhães, Marina Helena Cury Gallottini; Nunes, Fábio Daumas

doi:10.1590/s1806-83242005000400015

Cited by 10 publications

(5 citation statements)

References 16 publications

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“…This shows the persistent location of the virus with virus particles released at saliva. 16 EBV expression of LMP1 and integral membrane protein have been detected in OHL. LMP1 will alter cell function by inducing cellular gene expression and in epithelial cells by inducing EGFR expression.…”

Section: Discussionmentioning

confidence: 99%

“…Specific, sensitive, and new molecular epidemiological techniques have been developed to identify EBV genes, namely LMP1 gene-sequence variation patterns. 16 LMP1 is the main EBV oncoprotein, its expression is needed for EBV latent infection and B cell's transformation. 14 EBV infection in lymphocytes B generally occurs nonlytically and gives expression to membrane proteins and nuclear proteins.…”

Section: Introductionmentioning

confidence: 99%

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Detection of Epstein-Barr Virus in Saliva and Gen LMP1 among HIVInfected Patients HIVInfected Patients

Munth¹,

Wisaksana²,

Amalia³

et al. 2019

J Dent Indones

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Epstein-Barr virus (EBV) is also called human herpes virus 4 (HHV-4), has detected 95% of the population and shows an asymptomatic state. EBV is etiological agent of oral hairy leukoplakia (OHL) in HIV patients. Latent membrane protein 1 (LMP1), an integral EBV protein can modulate growth, differentiation, induce the expression of several cells, activation of antigens, and adhesion molecules. The LMP1 gene has been associated with OHL. Objectives: to determine the prevalence of EBV in saliva and the LMP1 gene in HIV/AIDS patients with EBV positive. Methods: A cross-sectional was conducted on HIV/AIDS patients. The presence of EBV in saliva was done by mciroarray PCR. LMP1 is examined by using nested PCR. Results: The research subjects involved 30 HIV/AIDS patients consisting 70% men and 30% women, with 50 % age group of 31-40 years old and 40% had CD4 counts <200 cells/mm 3 (40%). EBV in saliva was found in 26 out of 30 (87%) HIV patients and LMP1 was detected in 17 patients (65.38%). Conclusion: The high prevalence of EBV in saliva and the LMP1 gene may increase the risk of OHL. Early screening for EBV infection in patients with HIV/AIDS is important to reduce the risk of EBV-associated diseases.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection of Epstein-Barr Virus in Saliva and Gen LMP1 among HIVInfected Patients HIVInfected Patients

Munth¹,

Wisaksana²,

Amalia³

et al. 2019

J Dent Indones

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“…Oral hairy leukoplakia (OHL) presents as white patches, which are observed normally on the lateral surfaces of the tongue (Komatsu et al, 2005). The presence of EBV is detected in the tissues and blood due to chronic immunosuppression like HIV infection (De Paschale and Clerici, 2012).…”

Section: Ebv Associated Diseasesmentioning

confidence: 99%

Epstein–Barr Virus: Diseases Linked to Infection and Transformation

Jha

Pei

2016

Front. Microbiol.

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Epstein–Barr virus (EBV) was first discovered in 1964, and was the first known human tumor virus now shown to be associated with a vast number of human diseases. Numerous studies have been conducted to understand infection, propagation, and transformation in various cell types linked to human diseases. However, a comprehensive lens through which virus infection, reactivation and transformation of infected host cells can be visualized is yet to be formally established and will need much further investigation. Several human cell types infected by EBV have been linked to associated diseases. However, whether these are a direct result of EBV infection or indirectly due to contributions by additional infectious agents will need to be fully investigated. Therefore, a thorough examination of infection, reactivation, and cell transformation induced by EBV will provide a more detailed view of its contributions that drive pathogenesis. This undoubtedly expand our knowledge of the biology of EBV infection and the signaling activities of targeted cellular factors dysregulated on infection. Furthermore, these insights may lead to identification of therapeutic targets and agents for clinical interventions. Here, we review the spectrum of EBV-associated diseases, the role of the encoded latent antigens, and the switch to latency or lytic replication which occurs in EBV infected cells. Furthermore, we describe the cellular processes and critical factors which contribute to cell transformation. We also describe the fate of B-cells and epithelial cells after EBV infection and the expected consequences which contribute to establishment of viral-associated pathologies.

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“…This can be especially useful in early stages of the disease or when the manifestations are not characteristic, thus allowing early diagnosis and treatment. 72,73 72,73 …”

Section: Pcr and Viral Pathogensmentioning

confidence: 99%

Infections in Dermatopathology

Abbas

Bhawan

2012

The American Journal of Dermatopathology

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Significant difficulties continue to exist in the diagnosis of many infectious diseases in dermatopathology. Identifying pathogens in skin biopsies using conventional diagnostic techniques such as microscopy and tissue culture has its limitations. In recent times, molecular techniques including immunohistochemistry, polymerase chain reaction, and in situ hybridization have emerged as useful tools in the diagnosis of cutaneous infections that are challenging and ambiguous on conventional histopathology, thus improving our diagnostic accuracy. In this review, we highlight the main molecular techniques that have been used and continue to evolve in the diagnosis of mucocutaneous infectious diseases and discuss their applicability.

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Epstein-Barr virus in oral hairy leukoplakia scrapes: identification by PCR

Cited by 10 publications

References 16 publications

Detection of Epstein-Barr Virus in Saliva and Gen LMP1 among HIVInfected Patients HIVInfected Patients

Detection of Epstein-Barr Virus in Saliva and Gen LMP1 among HIVInfected Patients HIVInfected Patients

Epstein–Barr Virus: Diseases Linked to Infection and Transformation

Infections in Dermatopathology

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