In vitro biocompatibility tests of two commercial types of mineral trioxide aggregate

Abstract

Recently, regular and white mineral trioxide aggregate (MTA) are being used in Dentistry as retrofilling materials. Genotoxicity and cytotoxicity tests form an important part of cancer research and risk assessment of potential carcinogens. Thus, the goal of this study was to examine the genotoxicity and cytotoxicity of regular and white MTA in vitro by the single cell gel (comet) assay and trypan blue exclusion test, respectively. Mouse lymphoma cells were exposed to two presentation forms of MTA at final concentrations ranging from 1 to 1,000 microg/mL for 3 h at 37 degrees C. The results showed that both compounds tested did not produce genotoxic effects at all concentrations evaluated. Likewise, no statistically significant differences (p > 0.05) were observed in cytotoxicity. Taken together, our results suggest that regular and white MTA are not genotoxins and are not able to interfere in cellular viability as assessed by single cell gel (comet) assay and trypan blue assay, respectively.

Genotoxicity and cytotoxicity of mineral trioxide aggregate and calcium enriched mixture cements on L929 mouse fibroblast cells

“…Genotoxicity assays are of special importance in evaluating biocompatibility, because they have achieved general acceptance as serious and useful indicators of carcinogenicity 15) . Many different systems for detecting genotoxicity have been developed.…”

“…Animal systems may more accurately reflect human metabolism; however, they are expensive and time-consuming (requiring up to 3 years to complete); they are, therefore, not used as a first screen for genotoxicity or carcinogenicity. Thanks to the work of previous investigators, the single-cell, gel (comet) assay has been accepted as a rapid, simple, and reliable method of evaluating the genotoxicity of materials [15][16][17][18][19][20] . The basic principle of this assay is the migration of DNA fragments in an agarose matrix under electrophoresis.…”

Evaluation of cytotoxicity and gelatinases activity in 3T3 fibroblast cell by root repair materials

“…Amongst in vitro assays, in vitro cytotoxic tests are simple, reproducible, relevant and suitable for evaluation of basic biologic properties. [7] Cytotoxicity can be determined using the MTT-based colorimetric assay. MTT is 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and exists as a yellow tetrazolium salt.…”

Effect of additives on the compressive strength and setting time of a Portland cement

“…2,4,5 Dental applications of MTA and PCs have been largely investigated, and it has since been suggested that they fulfill many of the ideal properties of endodontic materials, such as biocompatibility, good sealing and marginal adaptation. 1,[6][7][8][9][10][11] Despite such favorable biological properties, the indication of MTA and PCs for restorative purposes requires improvements in their chemical and mechanical properties. 4,6 Compressive strength and setting time of endodontic cements are critical for optimized clinical performance, and thus represent a significant advance for their use as restorative materials.…”