In vitro activity of zinc oxide-eugenol and glass ionomer cements on Candida albicans

Abstract

The aim of this study was to evaluate in vitro the antimicrobial activity of glass ionomer (GIC) and zinc oxide-eugenol (ZOE) cements against Candida albicans. Standardized GIC and ZOE specimens were maintained in contact with C. albicans suspension (1 x 10(6) cells/ml) at 37 degrees C for 24 h, 48 h or 7 days. A control group without any testing cement was included. After the incubation period, aliquots of 0.1 ml were plated on Sabouraud's agar, and then the number of colonies was counted. The results were expressed as values of logarithms of colony-forming units per milliliter (log CFU/mL) and were analyzed statistically by Kruskal-Wallis ANOVA. After 48 h of incubation, the ZOE group presented no growth of C. albicans. GIC and control groups presented similar mean values at all tested periods. According to the results obtained, it could be concluded that, under the experimental conditions, ZOE cement was more effective in vitro against C. albicans than GIC.

Untitled

“…The ZnO-eugenol cement was more effective in reducing Candida spp. colony than the glass ionomer cement, with zerocounts after 48 h 31,34 , whereas the present study implies that ZnO could inhibit about 50% of the C. albicans after 4 h. All these results support the antifungal activity of ZnO. Many studies suggest that the release of highly active free radicals after ZnO activated with light radiation may be a mechanism responsible for antibacterial activity 35 .…”

“…ZnO inhibited about 90% of S. aureus growth and about 50% of C. albicans growth at concentration of 5 mg/10 ml, whereas ZnO inhibited E. coli in a dose dependent fashion from 0-100 mg/10 ml ZnO and the highest inhibition was 50% of E. coli growth. Many reports showed that ZnO could be used as antimicrobial agent [29][30][31][32] , as it exhibited antimicrobial activity against S. aureus 29 and E. coli 7,30 . The antibacterial activity of ZnO increased with the concentration 29 in agreement with the present investigation of anti-E. coli.…”

Antibacterial activity of glass ionomer cement modified by zinc oxide nanoparticles

“…On the other hand, studies on the effects of zinc oxide nanoparticles associated with glass ionomer cement (GIC) are scarce (Spencer, Campbell, Buschang, Cai, & Honeyman, 2009), although such data would be important. GIC is a restorative material that shows good chemical adhesion to the dental structure, releases fluorine (Cassanho et al, 2005;Weng, Guo, Gregory, & Xie, 2010), enables restoration of fluorine after topical applications, alters acid production and the electrolytic metabolism of cariogenic microorganisms, and promotes a decrease in the number of S. mutans and plaque bacteria after restoration (Chau, Pandit, Cai, Lee, & Jeon, 2015). The fluorine released by GIC can alter the ecosystem of the bacterial plaque and affect a variety of enzymatic functions that are vital for the bacterial cell, including inhibition of enolase, phosphatase, and pyrophosphatase, eventually leading to cell death (Chau et al, 2015;Duque, Negrini, Hebling, & Spolidorio, 2005;Hotwani, Thosar, Baliga, Bundale, & Sharma, 2013).…”

Cytotoxic and antifungal studies of biosynthesized zinc oxide nanoparticles using extract of Prosopis farcta fruit

“…Previous studies have shown that cumulative masses of Candida are strongly resistant to drugs; therefore, to prevent the accumulation, growth and proliferation of these fungi at tissue surface or inorganic levels, it is necessary to identify and introduce antifungal agents that control or inhibit the growth of fungal elements (12). ZnO NPs are highly active against bacteria and can be used as antifungal agent (13,14).…”