Effect of different dye solutions on the evaluation of the sealing ability of mineral trioxide aggregate

Abstract

Alkaline materials have shown incompatibility with methylene blue dye in leakage experiments. The goal of the present study was to analyze the effect of different dyes on the evaluation of the apical sealing ability of Mineral Trioxide Aggregate root-end fillings. Fifty-six extracted human canines were submitted to root canal instrumentation and obturation. After apical resection, retrograde cavities were prepared and teeth were randomly divided into four experimental (n = 13) and two control groups (n = 2). The following root-end filling materials were used: groups 1 and 2--Pro Root MTA (Dentsply), groups 3 and 4--zinc oxide-eugenol cement (ZOE). Teeth in groups 1 and 3 were immersed in 2% methylene blue solution, while teeth in groups 2 and 4 were immersed in 0.2% rhodamine B in a reduced pressure environment for 48 hours. Teeth were then longitudinally sectioned and leakage was evaluated. Results were submitted to statistical analysis (ANOVA and Tukey's test). Group 1 presented the least leakage (p < 0.05). It was concluded that the evaluation of the sealing ability of MTA is influenced by the dye used, since this material presented better sealing ability when evaluated with Methylene Blue, but was similar to ZOE when evaluated with rhodamine B.

Reliability of assessing dye penetration along root canal fillings using methylene blue

“…(3). MTA alkalinity was also described as responsible for the lower MB penetrability compared with RB in previous studies (9,10).…”

“…Tanomaru Filho et al. also suggested RB in place of MB for investigating dye penetration on MTA‐based materials (9).…”

Papillary reconstruction and guided tissue regeneration for combined periodontal–endodontic lesions caused by palatogingival groove and additional root: a case report

“…Mineral trioxide aggregate possesses excellent sealing ability [34], biocompatibility [35,36], and antibacterial property [37], especially due to blood contamination [38]. It can also promote the regeneration of alveolar bone [39] and cementum [40,41].…”

Back‐scattered electron imaging for leakage analysis of four retrofilling materials

“…Marginal leakage studies have been conducted with human saliva (Al-Hezaimi et al, 2005), fluid filtration (Yildirim et al, 2008), and dye solutions such as India ink (Jenkins et al, 2006) and blue methylene (Aqrabawi, 2000). However, the use of dyes has been questioned to evaluate the marginal leakage, because the sealing capacity of the material may be influenced by the dye solution, producing results that are not reproducible and that have high standard deviations (Editorial Board of the Journal of Endodontics, 2007;Tanomaru-Filho et al, 2005;Wu et al, 1998).…”