Expression of VINV1, SCB1 and SCS1 genes in the seed coats of contrasting soybean seeds1

Abstract

-The seed coat is vital for the proper development of soybean seeds and also, after their physiological maturity, for providing protection against various biotic and abiotic factors. The aim of this work was to evaluate the relative expression of three candidate genes, VINV1, SCB1 and SCS1, which are possibly involved in seed quality, in the contrasting seed coats of four soybean genotypes. Two yellow seed coat genotypes, BMX Potência RR and CD 202, and two black seed coat genotypes, TP and IAC, were used. Relative gene expression was evaluated by the qPCR technique for seven phases of seed coat development in the four genotypes, at 25, 30, 35, 40, 45, 50 and 55 days after anthesis. The design was completely randomised, with three replications. Data were subjected to variance analysis and the means were compared by Tukey test at 5% probability. The VINV1 and SCS1 genes exhibit greater expression in the early stages of seed coat development in the cultivar BMX Potência RR. The SCB1 gene displays greater expression in the cultivar CD 202, also in the early stages of seed coat development. The three genes under study show greater expression in yellow seed coat genotypes.Key words: Soybean. Coats. Candidate genes.RESUMO -O tegumento é vital para o adequado desenvolvimento das sementes de soja e, também, após a maturidade fisiológica destas, conferindo proteção contra os fatores bióticos e abióticos. O objetivo do trabalho foi avaliar a expressão relativa de três genes candidatos, VINV1, SCB1 e SCS1, possivelmente envolvidos com a qualidade das sementes, em tegumentos contrastantes de quatro genótipos de soja. Foram utilizados dois genótipos de tegumentos amarelos, BMX Potência RR e CD 202, e dois genótipos de tegumentos pretos, TP e IAC. A expressão relativa dos genes foi avaliada pela técnica qPCR, em sete fases de desenvolvimento dos tegumentos dos quatro genótipos, aos 25; 30; 35; 40; 45; 50 e 55 dias após a antese. O delineamento foi o inteiramente casualizado, com três repetições. Os dados foram submetidos à análise de variância e as médias comparadas pelo teste de Tukey, ao nível de 5% de probabilidade. Os genes VINV1 e SCS1 apresentam maior expressão nas fases iniciais de desenvolvimento dos tegumentos da cultivar BMX Potência RR. O gene SCB1 apresenta maior expressão na cultivar CD 202, também nas fases iniciais de desenvolvimento dos tegumentos. Os três genes estudados têm maior expressão nos genótipos de tegumentos amarelos.Palavras-chave: Soja. Tegumentos. Genes candidatos.*Autor para correspondência 1 Recebido para publicação em 21/02/2014; aprovado em 24/10/2014 Pesquisa relacionada à Tese de Doutorado do primeiro autor, com auxílio da FAPERGS, CNPq e CAPES

Hybrid Genome Assembly of a Major Quantitative Disease Resistance Locus in Soybean Toward Fusarium graminearum

“…The subtilisin gene contains a putative partial deletion in the subtilisin gene flanking the B cluster of the I locus identified through no PCR amplification and no contigs assembled in this region of gene 27 in the Wyandot assembly. Studies by Batchelor et al (2000) and Bahry and Zimmer (2015) have shown seed coat subtilisin 1 ( SCS1 ) to be highly expressed in the seed coat of soybean seeds. SCS1 is known to be associated with formation and differentiation of parenchyma cells derived from the inner seed coat.…”

“…Our results show that removal of the seed coat causes highly resistant lines to become susceptible to F. graminearum , indicating that proteins or secondary compounds in the seed coat may be playing a role in resistance. SCS1 is differentially expressed in the seed coat (Batchelor et al, 2000; Bahry and Zimmer, 2015), there were two overexpressed subtilisin genes (subtilisin‐like protease and SUB1 homolog precursor) in the resistant compared with the susceptible parent, and there is putative partial deletion in the SUB1 homolog in Wyandot. These findings indicate potential roles for one or both of these genes in resistance to F. graminearum .…”