Resistance profile of strains of Mycobacterium fortuitum isolated from clinical specimens

Santos, Debora Ribeiro de Souza; Lourenço, Maria Cristina S.; Coelho, Fábrice Santana; Mello, Fernanda C. Q.; Duarte, Rafael Silva

doi:10.1590/s1806-37562016000000073

Cited by 18 publications

(10 citation statements)

References 9 publications

(17 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In other animals, such as cattle, M. fortuitum can cause chronic mastitis [ 12 ]. In addition, infections caused by M. fortuitum are characterized by high resistance to antibiotics, such as aminoglycosides, beta-lactams, macrolides, rifamycins, and tetracyclines [ 13–15 ]. Recently, a genomic study revealed that a set of genes associated with resistance to these antibiotics (a ph(3’’)-Ic , aac(2’)-Ib , arr -1, bla F, erm 39, rbp A, rox , and tap ) appears to be ubiquitous in M. fortuitum species [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Multidrug-resistant Mycolicibacterium fortuitum infection in a companion cat (Felis silvestris catus) in Brazil

Morgado

Ramos

Pereira³

et al. 2022

Access Microbiology

View full text Add to dashboard Cite

Mycolicibacterium fortuitum is a fast-growing bacterium and an opportunistic pathogen implicated in human and animal infections. Here we report the first case and genetic characterization of a strain of M. fortuitum isolated from skin lesions of a companion cat with atypical cutaneous mycobacteriosis in Brazil. In addition, the genome of this strain was sequenced, representing the first genome of this opportunistic pathogen isolated from an animal infection. The in silico and in vitro analysis regarding antibiotic resistance of this strain showed an intrinsic multiresistance antibiotic profile. However, this strain showed sensitivity to amikacin and ciprofloxacin, and the cat was treated long-term with these drugs.

show abstract

Section: Introductionmentioning

confidence: 99%

Multidrug-resistant Mycolicibacterium fortuitum infection in a companion cat (Felis silvestris catus) in Brazil

Morgado

Ramos

Pereira³

et al. 2022

Access Microbiology

View full text Add to dashboard Cite

show abstract

“…These organisms have not become resistant, and they are intrinsically resistant to most of the antibiotics that are currently available 11. Also, infections caused by M. fortuitum isolates need long-term antibiotic therapy, because of their high resistance to chemotherapeutic agents and disinfectants 12. The antibiotic of choice for infections caused by M. fortuitum is amikacin, but resistance to aminoglycosides and macrolides has also been reported 12,13.…”

Section: Introductionmentioning

confidence: 99%

“…Also, infections caused by M. fortuitum isolates need long-term antibiotic therapy, because of their high resistance to chemotherapeutic agents and disinfectants 12. The antibiotic of choice for infections caused by M. fortuitum is amikacin, but resistance to aminoglycosides and macrolides has also been reported 12,13. Therefore, there is a great need to develop new treatment regimens for RGM infections, especially infections due to M. fortuitum and M. abscessus 14.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of nontuberculous mycobacteria and high efficacy of D-cycloserine and its synergistic effect with clarithromycin against <em>Mycobacterium fortuitum </em>and <em>Mycobacterium abscessus</em>

Khosravi¹,

Mirsaeidi²,

Farahani³

et al. 2018

IDR

View full text Add to dashboard Cite

BackgroundThe prevalence of pulmonary disease caused by nontuberculous mycobacteria (NTM) is reportedly on the rise in the world. Some of the species are resistant to various antibiotics; hence, limited treatment options are available. The aims of this study were to investigate the prevalence of NTM and to determine the effect of d-cycloserine against Mycobacterium fortuitum and Mycobacterium abscessus isolated from clinical specimens to find out the synergistic effect of d-cycloserine and clarithromycin.MethodsA total of 95 nonduplicate pulmonary isolates of NTM were collected from three major Regional Tuberculosis (TB) Centers. NTM isolates were identified by conventional tests and PCR sequence analysis of the rpoB gene. PCR sequencing of erm-41 was performed for detecting the inducible resistance to macrolides. In vitro susceptibilities and activities of d-cycloserine-clarithromycin combinations were accessed using the broth microdilution method.ResultsAmong 714-positive acid-fast bacilli from TB-suspected cases, 95 isolates were identified as NTM (13.3%). The prevalence of identified isolates was as follows: M. fortuitum 46 (48.4%), Mycobacterium simiae 16 (16.8%), Mycobacterium kansasii 15 (15.7%), M. abscessus 7 (7.3%), Mycobacterium thermoresistibile 4 (4.2%), Mycobacterium elephantis 3 (3.2%), Mycobacterium porcinum 2 (2.1%), and Mycobacterium chimaera 2 (2.1%). In addition, rpoB sequence analysis could identify all NTM isolates. The effect of d-cycloserine was better than that of clarithromycin. The synergistic effect of d-cycloserine with clarithromycin was observed for six (100%) and five (71.5%) strains of M. fortuitum and M. abscessus, respectively.ConclusionIn the present study, we demonstrated a wide range of NTM in processed samples from different provinces of Iran. Our observations indicated that d-cycloserine was very active against M. abscessus and M. fortuitum; hence, d-cycloserine, either alone or in combination with clarithromycin, may be promising for the treatment of M. abscessus- and M. fortuitum-associated diseases.

show abstract

“…(4,5,6) M. fortuitum infections have been reported with a high prevalence of resistance to several drugs, including macrolides, betalactams, aminoglycosides and tetracyclines, in addition to antitubercular drugs (e.g., isoniazid, rifampin, ethambutol, clofazimine, ethionamide, and rifabutin). (7,8,9) So far, studies of M. fortuitum are mainly represented by case reports in humans and animals, some of them addressing drug susceptibility with a focus on a specific geographic region, so there is a gap in relation to the global epidemiological scenario and systematic analysis of M. fortuitum traits associated with pathogenicity. The availability of M. fortuitum genomic information and metadata associated with these organisms (e.g., host, geographic location, year) is an opportunity to begin to fill the gap in the general biological characteristics of this zoonotic and widespread opportunistic bacteria.…”

mentioning

confidence: 99%

Mycolicibacterium fortuitum genomic epidemiology, resistome and virulome

Morgado

Ramos

Freitas

et al. 2021

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

BACKGROUND Mycolicibacterium fortuitum is an opportunistic pathogen associated with human and animal infection worldwide. Studies concerning this species are mainly represented by case reports, some of them addressing drug susceptibility with a focus on a specific geographic region, so there is a gap in relation to the global epidemiological scenario.OBJECTIVES We aimed determine the global epidemiological scenario of M. fortuitum and analyse its traits associated with pathogenicity.METHODS Based on publicly available genomes of M. fortuitum and a genome from Brazil (this study), we performed a genomic epidemiology analysis and in silico and in vitro characterisation of the resistome and virulome of this species.FINDINGS Three main clusters were defined, one including isolates from the environment, human and animal infections recovered over nearly a century. An apparent intrinsic resistome comprises mechanisms associated with macrolides, betalactams, aminoglycosides and antitubercular drugs such as rifampin. Besides, the virulome presented Type VII secretion systems (T7SS), including ESX-1, ESX-3, ESX-4 and ESX-4-bis, some of which play a role on the virulence of Mycobacteriaceae species.MAIN CONCLUSIONS Here, M. fortuitum was revealed as a reservoir of an expressive intrinsic resistome, as well as a virulome that may contribute to its success as a global opportunist pathogen.

show abstract

Resistance profile of strains of Mycobacterium fortuitum isolated from clinical specimens

Cited by 18 publications

References 9 publications

Multidrug-resistant Mycolicibacterium fortuitum infection in a companion cat (Felis silvestris catus) in Brazil

Multidrug-resistant Mycolicibacterium fortuitum infection in a companion cat (Felis silvestris catus) in Brazil

Prevalence of nontuberculous mycobacteria and high efficacy of D-cycloserine and its synergistic effect with clarithromycin against <em>Mycobacterium fortuitum </em>and <em>Mycobacterium abscessus</em>

Mycolicibacterium fortuitum genomic epidemiology, resistome and virulome

Contact Info

Product

Resources

About