Based on the monthly runoff and meteorological data during 1959-2009 of the Dongjiang River Basin, the space-time change of meteorological elements and variation tendency of the runoff were analyzed by using the Mann-Kendall trend test, correlation analysis and relevancy analysis methods. The correlation and relevancy between them were also found out. The results showed that: 1) The precipitation and temperature decrease in summer and fall, while evaporation showed a significant reduction in each season; 2) The seasonal spatial distribution of precipitation and temperature is obvious, while that of evaporation distribution is relatively consistent; 3) The correlation coefficients of evaporation and precipitation between seasonal runoff are larger than that of temperature, and the relevance of evaporation with runoff in the four seasons are the smallest. The result can elaborate the response relationship between seasonal change of meteorological elements and runoff, which provides a basis for water resources planning and evaluation.