Correlação entre mediadores inflamatórios na secreção nasofaríngea e no soro de crianças com infecção do trato respiratório inferior por vírus sincicial respiratório e a gravidade da doença

Vieira, Ricardo P.; Diniz, Edna Maria de Albuquerque; Ceccon, Maria Esther Jurfest Rivero

doi:10.1590/s1806-37132010000100011

Cited by 32 publications

(14 citation statements)

References 25 publications

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“…TNF-α is an acute-phase, proinflammatory cytokine that has been shown to be increased in nasopharyngeal secretions at the time of hospitalization for RSV infection in infants [30]. In a previous study, we observed that an increase in TNF-α was shown to occur in inoculated lambs at day 3, but returned to control levels by day 6 p.i.…”

Section: Discussionmentioning

confidence: 85%

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Human Respiratory Syncytial Virus Memphis 37 Grown in HEp-2 Cells Causes more Severe Disease in Lambs than Virus Grown in Vero Cells

Derscheid

Geelen

McGill

et al. 2013

Viruses

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Respiratory syncytial virus (RSV) is the most common cause of bronchiolitis in infants and young children. A small percentage of these individuals develop severe and even fatal disease. To better understand the pathogenesis of severe disease and develop therapies unique to the less-developed infant immune system, a model of infant disease is needed. The neonatal lamb pulmonary development and physiology is similar to that of infants, and sheep are susceptible to ovine, bovine, or human strains of RSV. RSV grown in Vero (African green monkey) cells has a truncated attachment G glycoprotein as compared to that grown in HEp-2 cells. We hypothesized that the virus grown in HEp-2 cells would cause more severe clinical symptoms and cause more severe pathology. To confirm the hypothesis, lambs were inoculated simultaneously by two different delivery methods (intranasal and nebulized inoculation) with either Vero-grown or HEp-2-grown RSV Memphis 37 (M37) strain of virus to compare viral infection and disease symptoms. Lambs infected with HEp-2 cell-derived virus by either intranasal or nebulization inoculation had significantly higher levels of viral RNA in lungs as well as greater clinical disease including both gross and histopathologic lesions compared to lambs similarly inoculated with Vero-grown virus. Thus, our results provide convincing in vivo evidence for differences in viral infectivity that corroborate previous in vitro mechanistic studies demonstrating differences in the G glycoprotein expression by RSV grown in Vero cells.

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Section: Discussionmentioning

confidence: 85%

“…Various types of nebulizers and types of nebulization delivery (increased volumes with various titers, longer duration) may further affect the degree of disease severity. Our recent work demonstrates that nebulized delivery of RSV M37 grown in HEp-2 cells causes consistent disease with high titers in bronchoalveolar lavage fluid [30,31,32]. …”

Section: Discussionmentioning

confidence: 99%

Human Respiratory Syncytial Virus Memphis 37 Grown in HEp-2 Cells Causes more Severe Disease in Lambs than Virus Grown in Vero Cells

Derscheid

Geelen

McGill

et al. 2013

Viruses

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show abstract

“…In their study, Fernandez et al discovered that higher levels of soluble interleukin-10 (IL-10) positively correlated with both disease severity and duration of supplemental oxygen in infants with acute RSV infection (95). A separate study confirmed this associate with increased levels of IL-10 in nasopharyngeal secretions (96), but the pathogenicity of this correlation has yet to be determined.…”

Section: Pathophysiology and Histology Of Rsv And Influenza A (H1n1) mentioning

confidence: 82%

Viral Infection in the Development and Progression of Pediatric Acute Respiratory Distress Syndrome

2016

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Viral infections are an important cause of pediatric acute respiratory distress syndrome (ARDS). Numerous viruses, including respiratory syncytial virus (RSV) and influenza A (H1N1) virus, have been implicated in the progression of pneumonia to ARDS; yet the incidence of progression is unknown. Despite acute and chronic morbidity associated with respiratory viral infections, particularly in “at risk” populations, treatment options are limited. Thus, with few exceptions, care is symptomatic. In addition, mortality rates for viral-related ARDS have yet to be determined. This review outlines what is known about ARDS secondary to viral infections including the epidemiology, the pathophysiology, and diagnosis. In addition, emerging treatment options to prevent infection, and to decrease disease burden will be outlined. We focused on RSV and influenza A (H1N1) viral-induced ARDS, as these are the most common viruses leading to pediatric ARDS, and have specific prophylactic and definitive treatment options.

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“…In children with PIV-positive ARI, IL-6 concentrations were found to be significantly elevated in nasal wash fluid (El Feghaly et al, 2010), and elevated serum IL-6 has been associated with prolonged hospital stays for RSV bronchiolitis (Vieira et al, 2010). In our study, IL-6 was significantly elevated only in HAE infected with PIV2 or PIV3 (except for a single time point for PIV1), and the kinetics of basolateral IL-6 secretion were similar to those observed for IFN-α: in PIV2 infected cells, basolateral IL-6 rose early and then waned whereas concentrations in PIV3 infected cells increased gradually through day 5 pi (Fig.…”

Section: Resultsmentioning

confidence: 99%

Human parainfluenza virus serotypes differ in their kinetics of replication and cytokine secretion in human tracheobronchial airway epithelium

et al. 2012

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Human parainfluenza viruses (PIVs) cause acute respiratory illness in children, the elderly, and immunocompromised patients. PIV3 is a common cause of bronchiolitis and pneumonia, whereas PIV1 and 2 are frequent causes of upper respiratory tract illness and croup. To assess how PIV1, 2, and 3 differ with regard to replication and induction of type I interferons, interleukin-6, and relevant chemokines, we infected primary human airway epithelium (HAE) cultures from the same tissue donors and examined replication kinetics and cytokine secretion. PIV1 replicated to high titer yet did not induce cytokine secretion until late in infection, while PIV2 replicated less efficiently but induced an early cytokine peak. PIV3 replicated to high titer but induced a slower rise in cytokine secretion. The T cell chemoattractants CXCL10 and CXCL11 were the most abundant chemokines induced. Differences in replication and cytokine secretion might explain some of the differences in PIV serotype-specific pathogenesis and epidemiology.

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Correlação entre mediadores inflamatórios na secreção nasofaríngea e no soro de crianças com infecção do trato respiratório inferior por vírus sincicial respiratório e a gravidade da doença

Cited by 32 publications

References 25 publications

Human Respiratory Syncytial Virus Memphis 37 Grown in HEp-2 Cells Causes more Severe Disease in Lambs than Virus Grown in Vero Cells

Human Respiratory Syncytial Virus Memphis 37 Grown in HEp-2 Cells Causes more Severe Disease in Lambs than Virus Grown in Vero Cells

Viral Infection in the Development and Progression of Pediatric Acute Respiratory Distress Syndrome

Human parainfluenza virus serotypes differ in their kinetics of replication and cytokine secretion in human tracheobronchial airway epithelium

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