Imunofenotipagem e rearranjo gênico em doenças pulmonares linfocíticas e linfoproliferativas

Ishikawa, Camila Cristina; Ab’Saber, Alexandre Muxfeldt; Parra, Edwin Roger; Lin, Chin Jia; Barbas, Carmen Sílvia Valente; Capelozzi, Vera Luíza

doi:10.1590/s1806-37132007000600004

Cited by 4 publications

(7 citation statements)

References 25 publications

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“…Existem métodos alternativos, como a remoção direta por meio do uso de banho-maria para derreter a parafina, ou mesmo a não desparafinização das amostras (13,17) .…”

Section: Desparafinizaçãounclassified

Fatores que interferem na qualidade do DNA extraído de amostras biológicas armazenadas em blocos de parafina

Scorsato¹,

Telles²

2011

J. Bras. Patol. Med. Lab.

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Os avanços tecnológicos e metodológicos da biologia molecular criaram a possibilidade de obter DNA de amostras teciduais estocadas em blocos de parafina, alternativa esta que permite estudos retrospectivos de grandes bancos de tecidos contendo inúmeras doenças raras. O objetivo deste trabalho foi a realização de uma breve revisão de alguns fatores inerentes à qualidade do produto extraído proveniente de blocos de parafina armazenados. Os processos envolvidos nas fases de prefixação, fixação, pós-fixação apresentam aspectos que são causa de resultados de produtos finais insatisfatórios. Na fase de prefixação, alterações bioquímicas influenciam na preservação das macromoléculas. As mudanças moleculares causadas pelos fixadores para evitar autólise celular podem ser um limitante no momento da extração de DNA. A qualidade do DNA obtido nas duas primeiras fases é considerada importante para os procedimentos de pós-fixação (extração e purificação do DNA). Existem na literatura vários protocolos, com diferentes passos que podem ser modificados, para a obtenção de DNA de material parafinado. Assim, a compreensão das reações em cada fase é importante para solucionar ou minimizar problemas, que influenciam na qualidade das macromoléculas de DNA.

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“…Existem métodos alternativos, como a remoção direta por meio do uso de banho-maria para derreter a parafina, ou mesmo a não desparafinização das amostras (13,17) .…”

Section: Desparafinizaçãounclassified

Fatores que interferem na qualidade do DNA extraído de amostras biológicas armazenadas em blocos de parafina

Scorsato¹,

Telles²

2011

J. Bras. Patol. Med. Lab.

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“…In other cases, a surgical biopsy should be performed in order to rule out lymphoma. (98) Pulmonary lymphomas Primary lymphomas of the lung are rare. One of the most common is MALToma, which is also referred to as "extranodal marginal zone B-cell lymphoma".…”

Section: Acute Interstitial Pneumoniamentioning

confidence: 99%

Padrões tomográficos das doenças intersticiais pulmonares difusas com correlação clínica e patológica

et al. 2008

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High-resolution computed tomography (HRCT) is the radiological imaging technique best suited to revealing changes in lung structure. Various HRCT findings, taken together, can represent typical patterns. These patterns, in conjunction with the anatomical distribution of findings and with clinical data, can narrow the differential diagnosis of diffuse interstitial lung disease and, in many cases, indicate the correct diagnosis with a high degree of accuracy. The most common HRCT patterns seen in cases of diffuse interstitial lung diseases are the nodular pattern, linear/reticular opacities, cystic lesions, ground-glass opacities and consolidations. This article reviews the correlations between HRCT patterns and pathologic findings, summarizing the most common causes, as well as detailing the methods of investigation employed in order to diagnose the most common types of chronic diffuse lung disease.Keywords: Lung diseases, interstitial/pathology; Tomography, X-Ray computed; Diagnostic techniques, respiratory system. ResumoA tomografia de alta resolução (TCAR) é a técnica de imagem radiológica que reflete mais de perto as alterações da estrutura pulmonar. Os vários achados tomográficos podem ser combinados para formar padrões típicos. Estes, conjuntamente com a distribuição anatômica dos achados, e com os dados clínicos, podem estreitar o diagnóstico das doenças intersticiais pulmonares difusas, e em vários casos sugerir o diagnóstico correto com alto grau de acurácia. Os padrões mais comuns das doenças intersticiais pulmonares difusas na TCAR são o nodular, linear e reticular, lesões císticas, opacidades em vidro fosco e consolidações. Este artigo revisa as correlações entre os padrões tomográficos na TCAR e os achados patológicos e resume as causas mais comuns e os métodos de investigação para se atingir um diagnóstico nas doenças pulmonares crônicas difusas mais comuns.Descritores: Doenças pulmonares intersticiais/patologia; Tomografia computadorizada por raios X; Técnicas de diagnóstico do sistema respiratório.* Study carried out in the

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“…[6] BALT—a type of Mucosa-associated lymphoid tissue (MALT) lymphoproliferative disease is a result of chronic stimulation: BALT reappears in adults with antigenic stimulation, such as infection, cigarette smoking, chronic inflammation, but the specific antigens are rarely identified, usually diagnosed without gene arrangement analysis. [5,11,12] …”

Section: Discussionmentioning

confidence: 99%

“…Differentiation between LIP and pulmonary MALT/BALT lymphoma is not clear-cut. [5,12] The distinct entities in accordance with the WHO classification unfortunately arise in mucosal sites where the lymphocytes are not normally present and non-self antigenic stimulation is high. Waldenstrom macroglobulinemia-like phenomena and tomographic/PET findings were observed here but without signs of humoral/cellular clonality and clear malignancy (immunofixation was negative, κ / λ not significant, and IgH-not clonally rearranged Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, the molecular gene rearrangement is occasionally tested. [5] The radiologic findings of the primary lymphoid lesions are often nonspecific and, contrary to lymphomatoid granulomatosis, positron emission tomography (PET) is not used as a diagnostic tool. [1,6,7] One case report of increased metabolic activity at PET is found in LIP—in nodules that are larger than 11 mm.…”

Section: Introductionmentioning

confidence: 99%

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A case report of lymphoid intestitial pneumonia in common variable immunodeficiency

2017

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Rationale:Lymphoid interstitial pneumonia (LIP) is a rare disease with lymphocytic infiltration of the alveolar interstitial and air spaces, sometimes classified as a clonal lymphoproliferative disease (LPD) with high prevalence in patients with immunodysregulation. Although association of mucosa-associated lymphoid tissue (MALT) lymphoma development with infectious agents has been well described, it is not so in the case of LIP. Attempts to demonstrate an infective cause by direct microbe detection have failed, but association with atypical specific immune response to opportunistic infectious agent has not been studied.Patient concerns and Diagnoses:We performed clinical, biochemical, and immunologic analysis of patients LIP that arises primarily from the common variable immune deficiency (CVID) with normal immunoglobulin class M (IgM) level and mild infectious course as a result of immunodysregulation. At the age of 13 multiple nodules, areas of consolidation were observed and LIP was confirmed by histological examination. The progression of the disease with massive splenomegaly (17→27 cm), lymphadenopathy soft tissue infiltration coincides with high standardized uptake value (SUV was 3.1–5.2), regulatory T cells decrease (CD4+25highFoxP3+ level −0.02%, i.e., 8 cells per 100 μL), oligoclonal gammapathy: very high IgM (3340 mg/dL) and β2-microglobulin (18.8 mg/L) level observed 10 years later.Immune response polarization was observed in humoral and cellular compartment -Th and Tc-dependent: 10.8% of lymphocytes are CD8high+CMV pp65-pentamer positive cells (Epstein–Barr virus-specific not observed). Specific immune response polarization correlates with negative immunofixation, light chains κ/λ = 2.84 and narrow, but non-monoclonal T cell receptor (TCR)/ B cell receptor (BCR) repertoire.Lessons:Taking everything into account, this case report shows that LIP is a consequence of immune-dysregulation in CVID, that is, Treg deficiency, narrow lymphocyte repertoire, and abnormal ability to respond to cytomegalovirus (CMV) antigens. It may be visualized by positron emission tomography (PET) and monitored by CMV-specific immune response, β2-microglobulin level, and IgM paraproteinaemia, but not by immunofixation and κ/λ ratio.

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Imunofenotipagem e rearranjo gênico em doenças pulmonares linfocíticas e linfoproliferativas

Cited by 4 publications

References 25 publications

Fatores que interferem na qualidade do DNA extraído de amostras biológicas armazenadas em blocos de parafina

Fatores que interferem na qualidade do DNA extraído de amostras biológicas armazenadas em blocos de parafina

Padrões tomográficos das doenças intersticiais pulmonares difusas com correlação clínica e patológica

A case report of lymphoid intestitial pneumonia in common variable immunodeficiency

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