Derrame pleural por micobactéria não tuberculosa

Seiscento, Márcia; Bombarda, Sidney; Carvalho, Adriana Castro de; Campos, José Ribas Milanez de; Teixeira, Lisete R.

doi:10.1590/s1806-37132005000500014

Cited by 3 publications

(1 citation statement)

References 14 publications

(14 reference statements)

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“…Mycobacterium kansasii is a slow-growing non-tuberculous mycobacterium (NTM) mainly found in aquatic environments that, differing from many other NTM, can cause human infections in both immunocompetent and immunocompromised individuals (Griffith et al, 2007). In susceptible immunocompetent individuals, the bacterium most frequently causes chronic pulmonary disease indistinguishable from tuberculosis (TB), resulting in the formation of cavities in more than 70% of cases, or, less frequently, in bronchiectasis or nodules; in contrast, in immunocompromised patients, the disseminated disease is most common (Seiscento et al, 2005;Matveychuk et al, 2012;Moon et al, 2015;Bakuła et al, 2018a;Goldenberg et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

A Murine Model of Mycobacterium kansasii Infection Reproducing Necrotic Lung Pathology Reveals Considerable Heterogeneity in Virulence of Clinical Isolates

et al. 2021

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Among non-tuberculous mycobacteria, Mycobacterium kansasii is one of the most pathogenic, able to cause pulmonary disease indistinguishable from tuberculosis in immunocompetent susceptible adults. The lack of animal models that reproduce human-like lung disease, associated with the necrotic lung pathology, impairs studies of M. kansasii virulence and pathogenicity. In this study, we examined the ability of the C57BL/6 mice, intratracheally infected with highly virulent M. kansasii strains, to produce a chronic infection and necrotic lung pathology. As a first approach, we evaluated ten M. kansasii strains isolated from Brazilian patients with pulmonary disease and the reference strain M. kansasii ATCC 12478 for virulence-associated features in macrophages infected in vitro; five of these strains differing in virulence were selected for in vivo analysis. Highly virulent isolates induced progressive lung disease in mice, forming large encapsulated caseous granulomas in later stages (120–150 days post-infection), while the low-virulent strain was cleared from the lungs by day 40. Two strains demonstrated increased virulence, causing premature death in the infected animals. These data demonstrate that C57BL/6 mice are an excellent candidate to investigate the virulence of M. kansasii isolates. We observed considerable heterogeneity in the virulence profile of these strains, in which the presence of highly virulent strains allowed us to establish a clinically relevant animal model. Comparing public genomic data between Brazilian isolates and isolates from other geographic regions worldwide demonstrated that at least some of the highly pathogenic strains isolated in Brazil display remarkable genomic similarities with the ATCC strain 12478 isolated in the United States 70 years ago (less than 100 SNPs of difference), as well as with some recent European clinical isolates. These data suggest that few pathogenic clones have been widely spread within M. kansasii population around the world.

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Section: Introductionmentioning

confidence: 99%

A Murine Model of Mycobacterium kansasii Infection Reproducing Necrotic Lung Pathology Reveals Considerable Heterogeneity in Virulence of Clinical Isolates

et al. 2021

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Occurrence of pathogenic environmental mycobacteria on surfaces in health institutions

Martins¹

2010

RRTM

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Abstract:The presence of environmental mycobacteria on surfaces in two public health institutions, namely a health center and a hospital in upstate São Paulo (Brazil), was identified by polymerase chain reaction-restriction enzyme analysis (PRA). The possible sources of contamination by these microorganisms were evaluated, contributing to epidemiology studies. Methods: From June 2005 to June 2006, a total of 632 samples were collected from exposed surfaces, such as washbasins, drinking fountains, and other accessible sites, and the mycobacteria present in the samples were isolated and cultured. Results: Sixty-five mycobacteria were isolated from the 632 samples; 47 of which were detected in samples from the health center and 18 in samples collected from the hospital. The isolates were identified by DNA restriction patterns obtained by PRA, and potentially pathogenic species were found to be prevalent among the identified mycobacteria. This study shows that the PRA technique can be employed as a fast and easy method for identification of nontuberculous mycobacteria in public areas. Conclusions:The isolation of environmental mycobacteria from the two health institutions demonstrates that these surfaces are reservoirs of potentially pathogenic mycobacteria and indicates the need for continuous maintenance and monitoring. These data will add to the study of the epidemiology of these microorganisms.

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Isolamento de micobactérias provenientes de amostras clínicas da região de Rio Claro: análise da frequência

Bertoletti,

Alves,

Chimara

et al. 2011

Rev Inst Adolfo Lutz

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Os agentes etiológicos da tuberculose pertencem ao Complexo Mycobacterium tuberculosis (MT), porém outras espécies do gênero e de importância médica, denominadas micobactérias não causadoras de tuberculose (MNT), são frequentemente isoladas. A identificação correta e rápida das MNT é obrigatória para o diagnóstico, auxiliando o clínico na conduta terapêutica. A frequência da MT e MNT foi avaliada no período de 2003 a 2009, analisando-se 6.460 amostras clínicas, sendo 5.696 (88,2%) culturas negativas para micobactéria, 715 (11,1%) culturas positivas e 49 (0,7%) não avaliadas. Os isolados mais frequentes foram MT 78,6% (562). Dentre as MNT, a espécie mais isolada foi M. gordonae em 3,09% (22) das amostras, seguida por M. fortuitum em 2,39% (17), Complexo M. avium (MAC) em 2,25% (16), M. kansasii em 1,97% (14) e M. abscessus em 1,83% (13). Quando relacionado ao número de pacientes, o complexo MAC foi o mais isolado (19,4%). Foi observado um declínio na frequência de isolamento de MT no período estudado, enquanto a frequência de MNT teve um aumento de 22,8%. A alta frequência de MT nas amostras é uma constante preocupação para a saúde pública, mas o aumento de doentes com MNT salienta a importância da notificação e do monitoramento epidemiológico desses pacientes.

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Derrame pleural por micobactéria não tuberculosa

Cited by 3 publications

References 14 publications

A Murine Model of Mycobacterium kansasii Infection Reproducing Necrotic Lung Pathology Reveals Considerable Heterogeneity in Virulence of Clinical Isolates

A Murine Model of Mycobacterium kansasii Infection Reproducing Necrotic Lung Pathology Reveals Considerable Heterogeneity in Virulence of Clinical Isolates

Occurrence of pathogenic environmental mycobacteria on surfaces in health institutions

Isolamento de micobactérias provenientes de amostras clínicas da região de Rio Claro: análise da frequência

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