Genotoxicity of Shallow Waters Near the Brazilian Antarctic Station "Comandante Ferraz" (Eacf), Admiralty Bay, King George Island, Antarctica

Abstract

Series of biomonitoring surveys were undertaken weekly in February 2012 to investigate the genotoxicity of the shallow waters around the Brazilian Antarctic Station "Comandante Ferraz" (EACF). The comet assay was applied to assess the damage to the DNA of hemocytes of the crustacean amphipods Gondogeneia antarctica collected from shallow waters near the Fuel Tanks (FT) and Sewage Treatment Outflow (STO) of the research station, and compare it to the DNA damage of animals from Punta Plaza (PPL) and Yellow Point (YP), natural sites far from the EACF defined as experimental controls. The damage to the DNA of hemocytes of G. antarctica was not significantly different between sites in the biomonitoring surveys I and II. In survey III, the damage to the DNA of animals captured in shallow waters near the Fuel Tanks (FT) and Sewage Treatment Outflow (STO) was significantly higher than that of the control site of Punta Plaza (PPL). In biomonitoring survey IV, a significant difference was detected only between the FT and PPL sites. Results demonstrated that the shallow waters in front of the station may be genotoxic and that the comet assay and hemocytes of G. antarctica are useful tools for assessing genotoxicity in biomonitoring studies of Antarctic marine coastal habitats.

Genotoxicity by Pahs In Shrimp (Litopenaeus Vannamei) and Its Impact on The Aquaculture of Two Coastal Ecosystems of The Gulf of California, Mexico

“…This can be due that shrimp in laboratory experiments, were exposed at sublethal concentrations, i.e., the experiments objective, was not to know genotoxicity vs. PAHs concentrations, such that has been, in other works [22]. However, the DNA damage (strand breaks) was similar or lower than values reported by other authors, where crustaceous or other estuarine invertebrates, were exposure to some PAHs [25], [26], [27].…”

Chapter 10. The Crab Ucides cordatus (Malacostraca, Decapoda, Brachyura) and Other Related Taxa as Environmental Sentinels for Assessment and Monitoring of Tropical Mangroves from South America

“…56 This is a more sensitive method to detect genomic microlesions, allowing individual identification of damage in most eukaryote cells, 57 and is used as an ecological indicator for diagnosis and monitoring of coastal areas. 58,59 Success has been obtained with this assay during environmental monitoring using vertebrates such as fishes, amphibians and mammals, as well as invertebrates such as decapod crustaceans. [60][61][62] The integrity of the lysosome membrane (neutral red retention time, NRRT) is an in vitro test that has been used as a chemical stress biomarker for the detection of xenobiotics' harmful effects in organelles.…”

Characterization of hemocytes from the marine amphipod Parhyale hawaiensis (Dana 1853): Setting the basis for immunotoxicological studies

“…Amphipods are an order of Crustacea widely used in ecotoxicology (Anderson et al, 2004; Ingersoll et al, 2002; X. Wang & Zauke, 2004; Weber et al, 2013). Several studies have used the hemolymph of amphipods to measure adverse effects, such as DNA damage (comet assay), from various types of exposures (Botelho et al, 2022; Lacaze et al, 2010; Rocha et al, 2015; Ronci et al, 2015); however, these studies provide minimal information about the cell types present in the hemolymph, or their proportions. The hemocytes have been briefly described only for Echinogammarus stammeri , a freshwater amphipod (Dezfuli et al, 2008).…”