Dynamics of ovarian maturation during the reproductive cycle of Metynnis maculatus, a reservoir invasive fish species (Teleostei: Characiformes)

Pereira, Thiago Scremin Boscolo; Moreira, Renata Guimarães; Batlouni, Sérgio Ricardo

doi:10.1590/s1679-62252013000400010

Cited by 15 publications

(18 citation statements)

References 43 publications

(59 reference statements)

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“…niloticus (Rocha and Reis-Henriques 1996;Costa Melo et al 2014), the spotted metynnis, Metynnis maculatus (Kner, 1858) (Pereira et al 2013) and the zebrafish, Danio rerio (Hamilton, 1822) (Connolly et al 2014). However, unlike them, late vitellogenic oocytes were not observed in the ovary of C. dimerus during the first days following spawning.…”

Section: Cichlasomamentioning

confidence: 99%

Dynamics of ovarian maturation throughout the reproductive cycle of the Neotropical cichlid fishCichlasomadimerus(Teleostei, Cichliformes)

et al. 2017

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In this study, we analyzed gene expression profiles, plasma steroids concentrations, and gonadal morphology throughout the reproductive cycle of female Cichlasoma dimerus (Heckel, 1840), a monogamous cichlid fish exhibiting social hierarchies. Fish were analyzed at six phases encompassing their annual cycle, namely resting (during the nonreproductive period), prespawning, 30 h post spawning, 4 days post spawning, 10 days post spawning, and subordinate (during the reproductive period). The histological and histomorphometric analysis showed that C. dimerus exhibits asynchronous ovarian development. Similar to resting females, subordinate females showed low gonadosomatic index, reduced expression levels of vitellogenin (vtgAb), zona pellucida (zpB), gonadal aromatase (cyp19a1A), and low concentrations of plasma sex steroids, thus indicating that social intimidation by dominant conspecifics elicited reproductive arrest. In reproductively active females, a direct positive correlation between plasma estradiol, vtgAb expression, percentage of late vitellogenic oocytes, and gonadosomatic index was observed. These parameters were maximal at the prespawning phase, decreased at 30 h post spawning and 4 days post spawning, and then reached a peak at 10 days post spawning. Our results indicate that female C. dimerus become spawning capable after 10 days post spawning, coincidently with the shortest time interval between successive spawns recorded in captivity.

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Section: Cichlasomamentioning

confidence: 99%

Dynamics of ovarian maturation throughout the reproductive cycle of the Neotropical cichlid fishCichlasomadimerus(Teleostei, Cichliformes)

et al. 2017

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“…Three fields from each region of the ovary (anterior, medial, and cranial; total of nine fields) were randomly selected, with a total of 2880 points scored for each animal at magnification 49. For this analysis, the methodology applied by Pereira et al (2013Pereira et al ( , 2016 was used with some modifications. Points were classified as one of the following: previtellogenic oocyte (PV), cortical alveoli oocyte (CA), immature oocytes with incomplete vitellogenesis and cytoplasm not fully filled with yolk (IV), mature vitellogenic oocytes with cytoplasm filled entirely by yolk and central nucleus (CNV), mature vitellogenic oocytes with cytoplasm filled entirely by yolk and migrated nucleus (MNV), mature vitellogenic oocytes with cytoplasm filled entirely by yolk and showing germinal vesicle break down (GVBD) and atretic oocyte (AT).…”

Section: Histomorphometric Analysesmentioning

confidence: 99%

The use of mGnRHa provokes ovulation but not viable embryos in Leporinus macrocephalus

et al. 2016

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In rheophilic tropical fish, the use of carp pituitary extract (CPE) is still the main protocol since the use of gonadotropin-releasing hormone (GnRH) induces ovulation, but usually does not provide viable embryos. Thus, in order to generate knowledge to support the establishment of a successful protocol using GnRH, we compared the ovulatory process and reproductive performance of Leporinus macrocephalus using CPE (0.5 and 5.0 mg kg-1) and mGnRHa (7 lg kg-1) associated with metoclopramide. Females of both treatments ovulated, but the mGnRHa treatment did not provide viable embryos, which was associated with a more potent ovulation, lower latency period and less intense hydration of eggs, suggesting a possible treatment overstimulation. This is the first report associating a less intense egg hydration process to a failure in obtaining viable eggs in induced fish spawning. Considering that the mGnRHa doses applied here were quite low, it is possible that L. macrocephalus as well as other rheophilic tropical species present a higher sensibility to mGnRHa and/or metoclopramide (or derived substances) and future approaches must consider using even lower doses of these substances as well as testing different types of GnRH and dopamine inhibitors.

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“…Increases in E2 levels are described during the vitellogenic period for several species (PEREIRA et al, 2013(PEREIRA et al, , 2016, including rheophilic fish (ARANTES et al, 2010;HAINFELLNER et al, 2012b). In this study, E2 elevations during the vitellogenic period (between March and October) were also recorded, but more sharply for OFs.…”

Section: Discussionmentioning

confidence: 99%

Seleção de fêmeas de pacu para indução hormonal: efeito da idade e dos métodos de avaliação

2016

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In this study, we investigated the reproductive cycle and spawning season of old (OFs) and young (YFs) P. mesopotamicus females, aiming to improve the process of selection of suitable fish for induced spawning. We also evaluated the accuracy of using external body characteristics in selecting suitable females. To that, 60 OFs and 60 YFs (10 and four years old, respectively) were submitted to seven samplings during two reproductive cycles. In each sampling, females (five to 10 per treatment) were randomly chosen to evaluation of plasma concentrations of gonadal steroids and ovary composition (biopsies and stereology). Females were also submitted to an external evaluation concerning the length of abdominal thickness, the degree of the abdominal rigidity and the aspect of urogenital papilla. In spite of OFs have shown reduced concentrations of 17α-hydroxyprogesterone during the spawning season, we observed that OFs and YFs kept in captivity had similar reproductive cycles and spawning seasons (in December for both groups). We do not recommend the exclusive use of external evaluation for selecting breeders to spawning induction due to a great inaccuracy of data and lack of association with histology and ovarian biopsy, which are more accurate and reliable. RESUMONeste estudo, investigamos o ciclo reprodutivo e a época de desova de fêmeas "velhas" (FVs) e fêmeas "jovens" (FJs) de P. mesopotamicus, visando melhorar o processo de seleção de peixes para a indução hormonal. Avaliamos também a acurácia do uso de características corporais externas para a seleção de fêmeas para indução hormonal. Para isso, 60 FVs e 60 FJs (10 e quatro anos, respectivamente) foram submetidas a sete amostragens durante dois ciclos reprodutivos. Em cada amostra, as fêmeas (cinco a 10 por tratamento) foram capturadas para avaliação das concentrações plasmáticas de esteroides gonadais e composição dos ovários (biópsias e estereologia). As fêmeas foram também submetidas à uma avaliação externa relativa à espessura abdominal, o grau de rigidez abdominal e o aspecto da papila urogenital. Exceto pela ocorrência de concentrações reduzidas de 17α-hidroxiprogesterona nas FVs, durante a época de desova, observamos que FVs e FJs, mantidas em cativeiro, apresentam ciclos reprodutivos e épocas de desova similares (em dezembro para ambos os grupos). Não recomendamos o uso exclusivo da avaliação externa para a seleção de reprodutores para indução a desova, devido a uma imprecisão dos dados e falta de associação destes com a histologia e biópsia de ovário, os quais são mais precisos e confiáveis.Palavras-chave: seleção de matrizes; desova induzida; biópsia ovariana; ciclo reprodutivo; falha na ovulação Artigo Científico: Recebido em 30

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Dynamics of ovarian maturation during the reproductive cycle of Metynnis maculatus, a reservoir invasive fish species (Teleostei: Characiformes)

Cited by 15 publications

References 43 publications

Dynamics of ovarian maturation throughout the reproductive cycle of the Neotropical cichlid fishCichlasomadimerus(Teleostei, Cichliformes)

Dynamics of ovarian maturation throughout the reproductive cycle of the Neotropical cichlid fishCichlasomadimerus(Teleostei, Cichliformes)

The use of mGnRHa provokes ovulation but not viable embryos in Leporinus macrocephalus

Seleção de fêmeas de pacu para indução hormonal: efeito da idade e dos métodos de avaliação

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