Study of sodium arsenite induced biochemical changes on certain biomolecules of the freshwater catfish Clarias batrachus

Abstract

Toxic impact of sublethal concentration (1 mg/L; 5% of 96h LC 50 value) of sodium arsenite (NaAsO 2 ) on certain biomolecules (proteins, nucleic acids, lipids, and glycogen) of five tissue components (muscles, liver, brain, skin, and gills) of the freshwater catfish Clarias batrachus was analysed. The important toxic manifestations include marked decrease in the concentration of proteins (21.72-45.42% in muscles; 3.42-53.94% in liver; 15.39-45.42% in brain; 15.40-4.00% in skin and 11.35-64.13% in gills), .01% in gills), .93% in skin and 21.47-44.38% in gills) and glycogen (24.00-51.72% in muscles; 49.11-72.45% in liver; 11.49-26.03% in brain; 26.13-38.05% in skin and 17.80-37.97% in gills). Excepting liver where the lipid content increases (15.82-24.13%), the fat content also showed depletion in their concentration (10.40-29.83% in muscles; 8.30-34.45% in brain; 8.94-31.47% in skin and 12.75-28.86% in gills), in the rest of the organ systems.Foi analisado o impacto tóxico da concentração subletal (1 mg/L; 5% do valor de LC50 de 96h) do arsenito de sódio (NaAsO2) sobre certas biomoléculas (proteinas, ácidos nucleicos, lipídios e glicogênio) de cinco tecidos (músculos, fígado, cérebro, pele e brânquias) do bagre Clarias batrachus. As manifestações tóxicas importantes incluiram o decréscimo acentuado na concentração de proteinas (21,72-45,42% nos músculos; 3,42-53,94% no fígado; 15,39-45,42% no cérebro; 15,40-4,00% na pele e 11,35-64,13% nas brânquias), DNA (0,55-22,95% nos músculos; 8,33-14,06% no fígado; 5,30-18,40% no cérebro; 13,57-52,80% na pele e 12,38-31,01% nas brânquias), RNA (42,[68][69][70][71][72][73][74][75][76]16% nos músculos; 10,75% no fígado; 5,05% no cérebro; 7,93% na pele e 21,47-44,38% nas brânquias) e glicogênio (24,00-51,72% nos músculos; 49,11-72,45% no fígado; 11,49-26,03% no cérebro; 26,13-38,05% na pele e 17,80-37,97% nas brânquias). Excetuando o fígado onde o conteúdo de lipídeos aumentou (15,82-24,13%), houve uma depleção na concentração de lipídeos no restante dos sistemas orgânicos (10,40-29,83% nos músculos; 8,30-34,45% no cérebro; 8,94-31,47% na pele e 12,75-28,86% nas brânquias).Key words: Glycogen, Lipids, Nucleic acids, Proteins, Toxicity.Banaras Hindu University, Eco-physiology Unit, Department of Zoology, Varanasi-221 005, India. randhir18bhu@gmail.com (RK), tkbzool@yahoo.co.in (TKB) IntroductionThere has been a continuous and alarming influx of arsenic to aquatic environment worldwide from both naturally occurring and anthropogenic sources (Goering et al., 1999). More than one hundred million people are at high risk of elevated arsenic exposure, mainly via drinking water, as well as by the air born metalloid in the areas with coal burning and industrial emissions. Consumption of the arsenic contaminated fishes collected from the polluted waters might also contribute to bioaccumulation of arsenic in human beings. Hence it is of immense importance to know the arsenic induced damages in the different organ systems of fishes used for human consumption. In an effort to achieve t...

Metabolic alterations in liver of fresh water fish, C. punctata exposed to arsenic: an adverse and adaptive response to the environment

“…Although much evidence were not observed in presence of Na2HAsO4 on the enhancement of triglyceride, the previous study reveals that arsenic, a potent toxic and heavy element causes the similar effects regarding the synthesis of lipids in liver of fresh water fish (Kumar and Banerjee, 2012). The lipid accumulation, as indicated by the increased number and size of red-stained lipid droplets, was detected in the liver of arsenic-treated fish (Li et al, 2016).…”

“…The increased synthesis of cholesterol also may induce higher liver weight and fatty liver and cause lipogenesis as demonstrated by Kumar and Banerjee (2012). In separate experiments, groups of fish were exposed to Na2HAsO4 for 1 h and cholesterol contents in liver were recorded.…”

Effects of Sub Lethal Concentrations of Potassium Permanganate on Protein Content of Freshwater Crab: Barytelphusa Guerini

“…The total protein content in the hepatopancreas, muscle and gill were found to decline in the sub-lethal concentration of potassium permanganate. Kumar et al (2012) reported that sodium arsenide decreased in the concentration of protein in catfish Clarius batractus. Mahajan and Zambare, (2001) reported decrease in protein contents in the fresh water bivalve Corbicula striatella after heavy metal stress most of the time.…”

Proteomics analysis of liver samples from puffer fish Takifugu rubripes exposed to excessive fluoride: An insight into molecular response to fluorosis

“…Puffer fish used in our experiments were 6-montholds, 15 ± 2 cm in length, 120 ± 10 g in weight, and all from the same batch. A fluoride ion concentration of 35 mg/L was selected for this proteomics experiment because this is the concentration known to induce fluorosis and also sublethal to the 16-cm catfish previously reported by Kumar et al [16]. Sixteen puffer fish were randomly divided into two groups, with eight fish in each group.…”

“…Fluoride can also induce endoplasmic reticulum (ER) stress and inhibit protein synthesis and secretion [14,15]. Recently, significant depletion of total protein and lipids has been observed in the liver of catfish after exposure to excessive fluoride [16]. Apoptosis and destruction of the normal cell cycle by fluoride have also been reported during organ culture of mouse fetal long bones [17].…”