Immunohistochemical detection of Lp25 and LipL32 proteins in skeletal and cardiac muscles of fatal human leptospirosis

Iglezias, Silvia D’Andretta; Abreu, Patrícia Antônia Estima; Kanamura, Cristina Takami; Magaldi, Antonio J.; Seguro, Antônio Carlos; Brito, Thales de

doi:10.1590/s1678-9946202062085

Cited by 3 publications

(2 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Discussionmentioning

confidence: 99%

“…The IHC positivity in Kupffer cells confirmed the presence of leptospiral antigen suspected by W&S and has been reported in humans with fatal leptospirosis using an antibody targeting the same epitope LipL32. 22 Limited antigen recognition could have been due to the large amounts of extravasated erythrocytes, neutrophils and fibrin that could have degraded and obscured the antigen. In addition, the best reactivity in IHC is with an antibody that is raised against the LPS of the serovar with which the animal is infected.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Exudative glomerulonephritis associated with acute leptospirosis in dogs

Hilbe,

Posthaus,

Paternoster

et al. 2023

Vet Pathol

View full text Add to dashboard Cite

In the past 20 years in Switzerland, dogs with suspect acute leptospirosis frequently showed severe glomerular changes that had not been previously reported. These features were characterized by abundant extravasated erythrocytes and fewer neutrophils accompanied by marked fibrin exudation into the urinary space that was interpreted as an exudative glomerulonephritis (GN). This retrospective study describes this significant glomerular pathological change and investigates the association with leptospirosis. Tissues from 50 dogs with exudative GN, retrieved from 2 pathology archives in Switzerland were reviewed using hematoxylin and eosin, periodic acid-Schiff, phosphotungstic acid-hematoxylin, and Warthin and Starry stains. Clinical and postmortem data were collected for each case. Immunohistochemistry (IHC) and/or polymerase chain reactions were used as confirmatory tests for leptospirosis. While all 50 cases had clinical and pathological features supporting a diagnosis of leptospirosis, 37 cases were confirmed for the disease. Using a LipL32 antibody in addition to the OMV2177 antibody raised against the lipopolysaccharide of Leptospira interrogans serovar Copenhageni increased the detection rate of Leptospira by IHC in exudative GN from 24% to 62%. Signalment, seasonality, clinical signs, blood results, and pathological changes in dogs with exudative GN were similar to those reported for dogs without GN and confirmed infection by Leptospira spp.. Exudative GN was common among Swiss dogs with leptospirosis where it caused acute severe disease. Leptospirosis should be considered as a cause of this new pathologic feature by the pathologist. The pathogenesis remains unclear, but involvement of a geographic-specific serovar with unique virulence factors is suspected and warrants further investigation.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Exudative glomerulonephritis associated with acute leptospirosis in dogs

Hilbe,

Posthaus,

Paternoster

et al. 2023

Vet Pathol

View full text Add to dashboard Cite

show abstract

Acute Leptospiral Myocarditis

Binayke¹,

Desai²,

Vaideeswar³

et al. 2022

Tropical Cardiovascular Pathology

View full text Add to dashboard Cite

Non-purulent myositis caused by direct invasion of skeletal muscle tissue by Leptospira in a hamster model

Miyahara,

Mori,

Fukuda

et al. 2024

Infect Immun

View full text Add to dashboard Cite

Myalgia is a common symptom of Leptospira infection in humans. Autopsies have reported that muscle tissue shows degeneration and necrosis of the myofibers and infiltration of inflammatory cells composed mainly of macrophages and lymphocytes. It remains unclear whether Leptospira directly infects the muscle and how the infiltrating inflammatory cells are involved in muscle fiber destruction. This study evaluated the relationship between histopathological changes and leptospiral localization in the muscle tissue of a hamster model. The influence of macrophages in skeletal muscle injury was also investigated, using selective depletion of macrophages by administration of liposomal clodronate. Hamsters infected subcutaneously with Leptospira interrogans serovar Manilae strain UP-MMC-SM showed myositis of the thighs adjacent to the inoculated area beginning at 6 days post-infection. The myositis was non-purulent and showed sporadic degeneration and necrosis of muscle fibers. The degeneration of myofibers was accompanied by aggregations of macrophages. Immunofluorescence staining revealed leptospires surrounding the damaged muscle fibers. Subcutaneous injection of formalin-killed Leptospira or intraperitoneal injection of live Leptospira caused no myositis in hamster thighs. Liposomal clodronate treatment in infected hamsters reduced macrophage infiltration in muscle tissue without impacting bacterial clearance. Muscle necrosis was still observed in the infected hamsters treated with liposomal clodronate, and there was no significant change in serum creatine kinase levels compared to those in animals treated with liposomes alone. Our findings suggest that leptospiral invasion of muscle tissue from an inoculation site leads to the destruction of muscle fibers and causes non-purulent myositis, whereas the infiltrating macrophages contribute less to muscle destruction.

show abstract

Immunohistochemical detection of Lp25 and LipL32 proteins in skeletal and cardiac muscles of fatal human leptospirosis

Cited by 3 publications

References 32 publications

Exudative glomerulonephritis associated with acute leptospirosis in dogs

Exudative glomerulonephritis associated with acute leptospirosis in dogs

Acute Leptospiral Myocarditis

Non-purulent myositis caused by direct invasion of skeletal muscle tissue by Leptospira in a hamster model

Contact Info

Product

Resources

About