First report of Raoultella ornithinolytica carrying blaKPC-2 isolated from a dipteran muscoid collected in a garbage from a public hospital in Rio de Janeiro, Brazil

Carramaschi, Isabel Nogueira; Castro, Edna Aparecida Barbosa de; Leite, Jéssica Albuquerque; Queiroz, Margareth Maria de Carvalho; Bôas, Maria Helena Simões Villas; Rangel, Karyne; Zahner, Viviane

doi:10.1590/s1678-9946201961032

Cited by 6 publications

(3 citation statements)

References 18 publications

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“…In addition, some resistant strains have also been isolated from animals, vegetables, wastewater, and river water. The major drug resistance genes identified in these isolates include bla NDM-1 , bla KPC-2 , mcr-1 , bla CTX-M-15 , bla IMP-4 , and bla OXA-1 (Zhou et al, 2014; Hasan et al, 2015; Zheng et al, 2015; Hernandez-Garcia et al, 2018; Miao et al, 2018; Yoon et al, 2018; Carramaschi et al, 2019; Wang et al, 2019). However, little is known regarding the carbapenemase- and ESBL-producing R. ornithinolytica strains.…”

Section: Discussionmentioning

confidence: 99%

Emergence of NDM-1- and CTX-M-3-Producing Raoultella ornithinolytica in Human Gut Microbiota

Wang

Chi

et al. 2019

Front. Microbiol.

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Raoultella ornithinolytica is an opportunistic pathogen of the Enterobacteriaceae family and has been implicated in nosocomial infections in recent years. The aim of this study was to characterize a carbapenemase-producing R. ornithinolytica isolate and three extended-spectrum β-lactamase (ESBL)-producing R. ornithinolytica isolates from stool samples of adults in a rural area of Shandong Province, China. The species were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and 16S rDNA sequence analysis. Antimicrobial susceptibility test showed that all four isolates were multidrug-resistant (MDR). The whole genome sequence (WGS) of these isolates was determined using an Illumina HiSeq platform, which revealed MDR-related genes. The S1 nuclease-pulsed-field gel electrophoresis (S1-PFGE) was used to characterize the plasmids carried by the R. ornithinolytica isolates. The blaNDM-1 and blaCTX-M-3 genes were probed using Southern blotting, which confirmed the location of both genes on the same plasmid with molecular weight of 336.5–398.4 kb. The transferability of blaNDM-1 and blaCTX-M was also confirmed by conjugation assays. Finally, BLAST analysis of both genes showed that mobile genetic elements were associated with the spread of drug resistance genes. Taken together, we report the presence of conjugative blaNDM-1 and blaCTX-M plasmids in R. ornithinolytica isolates from healthy humans, which indicate the possibility of inter-species transfer of drug resistance genes. To the best of our knowledge, this is the first study to isolate and characterize carbapenemase-producing R. ornithinolytica and ESBL-producing R. ornithinolytica isolates from healthy human hosts.

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Section: Discussionmentioning

confidence: 99%

Emergence of NDM-1- and CTX-M-3-Producing Raoultella ornithinolytica in Human Gut Microbiota

Wang

Chi

et al. 2019

Front. Microbiol.

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“…The first reported case of R. ornithinolytica resistance was in India, where metallo-β-lactamase-1 producing R. ornithinolytica was isolated from the wound in a patient with urethral injury [30]. Later, R. ornithinolytica antibiotic resistance has been reported in many countries like China [67], India [71] and Brazil [72].…”

Section: Antibiotic Treatment and Resistancementioning

confidence: 99%

A spotlight on Raoultella ornithinolytica: A newly emerging life-threatening zoonotic pathogen

El-Ghany

2021

Int J One Health

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The genus Raoultella contains Raoultella ornithinolytica (R. ornithinolytica), which was previously named as Klebsiella ornithinolytica. R. ornithinolytica is a Gram-negative bacillus that belongs to the family Enterobacteriaceae. This pathogen is normally present in aquaculture, and it has been isolated from fish, insects, and wild and domestic birds. Seafood and poultry products are incriminated as sources of R. ornithinolytica infection. Community-acquired human infection with R. ornithinolytica is common. This infection is commonly associated with sepsis, bacteremia, food poisoning, purities, and urinary and respiratory tracts' infections. Rapid acquired resistance of R. ornithinolytica strains is common, and it is related to the development of resistant genes. R. ornithinolytica is considered as a newly emerging life-threatening pathogen world-wide. Hence, it is very important to determine the role of animals and birds in the epidemiological situation of this pathogen as well as the detection of antibiotic resistance genes before treatment.

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Section: Antibiotic Treatment and Resistancementioning

confidence: 99%

January-July

2021

Int J One Health

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The genus Raoultella contains Raoultella ornithinolytica (R. ornithinolytica), which was previously named as Klebsiella ornithinolytica. R. ornithinolytica is a Gram-negative bacillus that belongs to the family Enterobacteriaceae. This pathogen is normally present in aquaculture, and it has been isolated from fish, insects, and wild and domestic birds. Seafood and poultry products are incriminated as sources of R. ornithinolytica infection. Community-acquired human infection with R. ornithinolytica is common. This infection is commonly associated with sepsis, bacteremia, food poisoning, purities, and urinary and respiratory tracts' infections. Rapid acquired resistance of R. ornithinolytica strains is common, and it is related to the development of resistant genes. R. ornithinolytica is considered as a newly emerging life-threatening pathogen worldwide. Hence, it is very important to determine the role of animals and birds in the epidemiological situation of this pathogen as well as the detection of antibiotic resistance genes before treatment.

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First report of Raoultella ornithinolytica carrying blaKPC-2 isolated from a dipteran muscoid collected in a garbage from a public hospital in Rio de Janeiro, Brazil

Cited by 6 publications

References 18 publications

Emergence of NDM-1- and CTX-M-3-Producing Raoultella ornithinolytica in Human Gut Microbiota

Emergence of NDM-1- and CTX-M-3-Producing Raoultella ornithinolytica in Human Gut Microbiota

A spotlight on Raoultella ornithinolytica: A newly emerging life-threatening zoonotic pathogen

January-July

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