In vitro effects of Blepharocalyx salicifolius (H.B.K.) O. Berg on the viability of Echinococcus ortleppi protoscoleces

Noal, Charlise Bolson; Monteiro, Danieli Urach; Brum, Thiele Faccim de; Emmanouilidis, Jéssica; Zanette, Régis A.; Morel, Ademir F.; Stefanon, Eliza Beti de Cassia; Frosi, Marina; Rue, Mario Luiz de la

doi:10.1590/s1678-9946201759042

Cited by 5 publications

(2 citation statements)

References 21 publications

(23 reference statements)

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“…In Brazil, it is known with the name of “Murta”, and is popularly used to treat respiratory diseases, coughs, colds, hypotension, rheumatism, hypoglycemia, diarrhea, leukorrhea, urethritis and bladder diseases [ 5 , 6 , 7 , 8 ]. Extracts of this species showed antiparasitic, antifungal, antibacterial, allelopathic, cytotoxic and insecticide effects [ 2 , 3 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Chemical Composition and Bioactivity of Essential Oil from Blepharocalyx salicifolius

Furtado

Borges

Teixeira

et al. 2018

IJMS

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Natural products represent a source of biologically active molecules that have an important role in drug discovery. The aromatic plant Blepharocalyx salicifolius has a diverse chemical constitution but the biological activities of its essential oils have not been thoroughly investigated. The aims of this paper were to evaluate in vitro cytotoxic, antifungal and antibacterial activities of an essential oil from leaves of B. salicifolius and to identify its main chemical constituents. The essential oil was extracted by steam distillation, chemical composition was determined by gas chromatography/mass spectrometry, and biological activities were performed by a microdilution broth method. The yield of essential oil was 0.86% (w/w), and the main constituents identified were bicyclogermacrene (17.50%), globulol (14.13%), viridiflorol (8.83%), γ-eudesmol (7.89%) and α-eudesmol (6.88%). The essential oil was cytotoxic against the MDA-MB-231 (46.60 μg·mL−1) breast cancer cell line, being more selective for this cell type compared to the normal breast cell line MCF-10A (314.44 μg·mL−1). Flow cytometry and cytotoxicity results showed that this oil does not act by inducing cell death, but rather by impairment of cellular metabolism specifically of the cancer cells. Furthermore, it presented antifungal activity against Paracoccidioides brasiliensis (156.25 μg·mL−1) but was inactive against other fungi and bacteria. Essential oil from B. salicifolius showed promising biological activities and is therefore a source of molecules to be exploited in medicine or by the pharmaceutical industry.

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Section: Introductionmentioning

confidence: 99%

Chemical Composition and Bioactivity of Essential Oil from Blepharocalyx salicifolius

Furtado

Borges

Teixeira

et al. 2018

IJMS

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“…Several studies demonstrated the antiparasitic activities of various EO of medicinal plants, in particular also against Echinococcus spp. (Maggiore et al ., 2012; Jahanbakhsh et al ., 2016; Moazeni et al ., 2017; Noal et al ., 2017; Sharifi-Rad et al ., 2018).…”

Section: Introductionmentioning

confidence: 99%

Activity of Thymus capitatus essential oil components against in vitro cultured Echinococcus multilocularis metacestodes and germinal layer cells

et al. 2019

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The essential oil (EO) of Thymus capitatus, seven fractions (F1–F7) obtained from silica gel chromatography, and several pure EO components were evaluated with respect to in vitro activities against Echinococcus multilocularis metacestodes and germinal layer (GL) cells. Attempts to evaluate physical damage in metacestodes by phosphoglucose isomerase (PGI) assay failed because EO and F1–F7 interfered with the PGI-activity measurements. A metacestode viability assay based on Alamar Blue, as well as transmission electron microscopy, demonstrated that exposure to EO, F2 and F4 impaired metacestode viability. F2 and F4 exhibited higher toxicity against metacestodes than against mammalian cells, whereas EO was as toxic to mammalian cells as to the parasite. However, none of these fractions exhibited notable activity against isolated E. multilocularis GL cells. Analysis by gas chromatography-mass spectrometry showed that carvacrol was the major component of the EO (82.4%), as well as of the fractions F3 (94.4%), F4 (98.1%) and F5 (90.7%). Other major components of EO were β-caryophyllene, limonene, thymol and eugenol. However, exposure of metacestodes to these components was ineffective. Thus, fractions F2 and F4 of T. capitatus EO contain potent anti-echinococcal compounds, but the activities of these two fractions are most likely based on synergistic effects between several major and minor constituents.

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Pharmacological Research Progress on Antimicrobial Infection of Gallic Acid

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2020

ACM

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In vitro effects of Blepharocalyx salicifolius (H.B.K.) O. Berg on the viability of Echinococcus ortleppi protoscoleces

Cited by 5 publications

References 21 publications

Chemical Composition and Bioactivity of Essential Oil from Blepharocalyx salicifolius

Chemical Composition and Bioactivity of Essential Oil from Blepharocalyx salicifolius

Activity of Thymus capitatus essential oil components against in vitro cultured Echinococcus multilocularis metacestodes and germinal layer cells

Pharmacological Research Progress on Antimicrobial Infection of Gallic Acid

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