Echinococcus granulosus sensu lato GENOTYPES IN DOMESTIC LIVESTOCK AND HUMANS IN GOLESTAN PROVINCE, IRAN

Sharbatkhori, Mitra; Tanzifi, Asal; Rostami, Sima; Rostami, Masoomeh; Harandi, Majid Fasihi

doi:10.1590/s1678-9946201658038

Cited by 35 publications

(29 citation statements)

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“…Echinococcus felidis was recovered as a sister taxon to G1-G3 genotypes in a distinct clade with maximum statistical support (pp = 1.00); this is in concordance with the studies of Lavikainen et al [65], Hüttner et al [66], Saarma et al [7] and previous studies in Iran [15,64]. Two E. granulosus haplotypes from the present study (E.gkh63 and E.gkh75) identified as the G1 genotype were grouped with reference G1 genotype within the cluster with G1-G3 genotypes with maximum statistical support (pp = 1.00).…”

Section: Discussionsupporting

confidence: 90%

“…Molecular analyses of echinococcosis in Iran have been performed mostly on CE in humans and domestic animals [15,19,20,22] and only to a limited extent on adult worms in the definitive hosts [24][25][26][27]. This difference mostly reflects difficulties related to field studies of definitive hosts, such as obtaining stray dogs and wild canids, contamination with viral infections (i.e.…”

Section: Discussionmentioning

confidence: 99%

“…To date, molecular studies on E. granulosus carried out in Iran have reported several genotypes in domestic livestock (genotypes G1-G7) [14][15][16][17][18] as well as in humans (G1-G3 and G6) from different endemic foci of Iran [19][20][21][22][23]. Additionally, the genotypes G1 [24][25][26][27], G2 [24], G3 [24][25][26] and G6 [25] have been identified in dogs in Iran.…”

Section: Introductionmentioning

confidence: 99%

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Echinococcus multilocularis and Echinococcus granulosus in canines in North-Khorasan Province, northeastern Iran, identified using morphology and genetic characterization of mitochondrial DNA

et al. 2019

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Background: Canids are definitive hosts of Echinococcus multilocularis and Echinococcus granulosus. This study aimed to survey these two Echinococcus species in canids of North-Khorasan Province, northeastern Iran, using morphological criteria and genetic characterization of mitochondrial DNA. Methods: The carcasses of 106 canids, namely 61 jackals (Canis aureus), 23 foxes (Vulpes vulpes), 19 dogs (Canis familiaris) and three wolves (Canis lupus) were collected from the study area in 2013-2014 and examined for Echinococcus species. Morphological features were assessed by microscopy of adult worms. For molecular characterization, DNA was extracted, mostly from the adult worms but also from eggs. DNA fragments of the cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nad1) mitochondrial genes were amplified and sequenced. Sequences were aligned and compared with reference sequences. Intraspecific and interspecific diversity were calculated and phylogenetic analysis was performed. Results: Overall, 9.4% of the canids (eight jackals and two foxes) were found infected with E. multilocularis by molecular methods, of which seven cases were also confirmed using morphological description of the adult worms. Echinococcus granulosus was found in 6.6% of the canines (four dogs, two jackals and one wolf) as determined by both molecular methods and adult cestode morphology. All E. granulosus isolates were identified as the G1 genotype. Comparative sequence analysis indicated 0-0.7% and 0% intraspecific divergence within E. granulosus isolates and 0% and 0-0.2% within E. multilocularis isolates for cox1 and nad1, respectively. Conclusions: This study revealed the presence of E. multilocularis and E. granulosus in canids of North-Khorasan Province of Iran. Jackals were found infected with both E. multilocularis and E. granulosus, but infection with the former species was higher.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Echinococcus multilocularis and Echinococcus granulosus in canines in North-Khorasan Province, northeastern Iran, identified using morphology and genetic characterization of mitochondrial DNA

et al. 2019

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“…In some studies, conducted in different geographical areas of Iran, the G1 strain of E. granulosus was reported as the dominance genotype in intermediate hosts including cattle, sheep, human and camels (37,38). In one study conducted in Golestan province, northern Iran, G1 and G3 strains have been reported in 78.3% and 15% of CE cases respectively (39). The G1 strain also reported from the wild boar in Iran (14).…”

Section: Discussionmentioning

confidence: 99%

Comparative Genotyping of <i>Echinococcus granulosus</i> Infecting Livestock in Turkey and Iran

Barazesh¹,

Sarkari²,

Sarısu³

et al. 2019

Turkiye Parazitol Derg

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Amaç: Echinococcus granulosus, yaşam döngüsü paterni ve konak tiplerine göre çeşitlilik gösteren farklı suşlara sahiptir. Şimdiye kadar bu parazitin 10 genotipi, moleküler yöntemler kullanılarak tespit edilmiştir. Bu çalışmada, Türkiye ve İran'daki hayvanlarda E. granulosus metasestodlarının genotipik çeşitliliğinin değerlendirilmesi ve karşılaştırılması amaçlanmıştır. Yöntemler: İran'ın Doğu Azerbaycan eyaletindeki Bonab şehrindeki (30 koyun ve 30 sığır dahil olmak üzere 60 örnek) ve Türkiye'nin Van şehrindeki (15 koyun ve 15 sığır dahil olmak üzere 30 örnek) endüstriyel kesimhanelerinden hidatik kist ile enfekte Objective: Echinococcus granulosus contains a complex of different strains that represent diversity in the pattern of the life cycle and also their host types. So far 10 genotypes of this parasite have been identified, using molecular methods. The current study aimed to evaluate and compare the genotypic diversity of E. granulosus metacestodes from livestock of Turkey and Iran. Methods: A total of 90 livestock liver and lung organs infected with hydatid cyst from industrial slaughterhouses of Bonab Province in the East Azerbaijan Province in Iran (60 samples, including 30 sheep and 30 cattle) and Van Province in Turkey (30 samples, including 15 sheep and 15 cattle) were collected. DNA was extracted from the protoscolices or germinal layers and polymerase chain reaction (PCR) were utilized, targeting the partial mitochondrial cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase 1 (nad1) genes. PCR products were isolated from the electrophoresis gels and sequenced. The sequences were compared with each other, as well as with those related available sequences in the GenBank, using the BioEdit software and the BLAST algorithm. Finally, the phylogenetic trees were constructed by comparing sequences of cox1 and nad1 fragments, using the MEGA7 software and the maximum likelihood method. Results: All samples sequenced from Iran corresponded to the genotype G1 (100%). Among the samples from Turkey, 15 samples (78.9%) were identified as G1 while only one sample (5.3%) corresponded to the genotype G3 and 3 isolates (15.8%) were defined as genotypes G1/G3. Five distinct haplotypes were determined within the examined isolates from sheep and cattle in both countries and all isolates clustered in one group. Phylogenetic analysis revealed that the intra-species genetic variations were 0.0-0.6% and 0.0-1.4% for cox1 and nad1, respectively. Conclusion: The dominant genotype of E. granulosus sensu stricto of livestock in both countries was the G1 (sheep strain) genotype. Our findings indicate that the sheepdog cycle is the leading cycle of E. granulosus in these two areas. Hence, adopting regional common policies and bilateral cooperation helps to control the disease in livestock as well as in human in these two regions. Further study is required to compare the genetic diversity of human isolates of E. granulosus in these two countries.

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“…Among EG s.s., one haplotype (HL1) was highly predominant in both humans and animals cysts (71.6 %) (Zait et al). Sharbatkhori et al (2016), of the Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran and other related centers, studied 74 hydatid cysts isolated from livestock (18 from sheep, 40 from cattle, 9 from camels, 2 from buffaloes and 1 from a goat) and humans (4 isolates) in the Golestan province, northern Iran, southeast of the Caspian sea, using partial sequencing data of COI and NADH dehydrogenase 1 mitochondrial genes. Then, isolates were analysed by PCR amplification and sequencing.…”

Section: Available Evidencementioning

confidence: 99%

Molecular Epidemiology of Cystic Echinococcosis: Genotypic Characterization in Humans and Different Livestock

Manterola

Otzen

2016

Int. J. Morphol.

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SUMMARY:Echinococcus Granulosus (EG) is the major cause of cystic echinococcosis in humans and livestock in the world. In Chile is a zoonosis of great importance. The most frequently affected geographic areas are the Regions of Aysén, Los Rios, Los Lagos, Coquimbo and the Araucanía. Hence, it was discovered that in endemic areas of hydatidosis there could be several strains and genotypes of EG. In addition, there is evidence that some strains and genotypes are more infectious for human beings than others. This interesting phenomenon of the biology of EG has been studied using molecular biology techniques based on polymerase chain reaction (PCR) and DNA sequence analysis, which has made it possible to characterize the cestode species complex called EG sensu lato (s l) as being comprised of EG sensu stricto (s.s.) (Genotypes G1-G3), E. equinus (G4), E. ortleppi (G5) and E. canadensis (G6-G10), which present an important phenotypic variation detectable in characteristics of the biological cycle, specificity of the intermediate host, pattern of development, pathogenicity, antigenicity, transmission dynamics and, consequently, in the measures needed to control the disease. The aim of this manuscript is to describe the different genotypes of EG described in humans and different livestock host reported in the literature.

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Echinococcus granulosus sensu lato GENOTYPES IN DOMESTIC LIVESTOCK AND HUMANS IN GOLESTAN PROVINCE, IRAN

Cited by 35 publications

References 41 publications

Echinococcus multilocularis and Echinococcus granulosus in canines in North-Khorasan Province, northeastern Iran, identified using morphology and genetic characterization of mitochondrial DNA

Echinococcus multilocularis and Echinococcus granulosus in canines in North-Khorasan Province, northeastern Iran, identified using morphology and genetic characterization of mitochondrial DNA

Comparative Genotyping of <i>Echinococcus granulosus</i> Infecting Livestock in Turkey and Iran

Molecular Epidemiology of Cystic Echinococcosis: Genotypic Characterization in Humans and Different Livestock

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