OCCURRENCE OF Calodium hepaticum (BANCROFT, 1893) MORAVEC, 1982 EGGS IN FECES OF DOGS AND CATS IN LAGES, SANTA CATARINA, BRAZIL

Abstract

This study aims to report the incidence of Calodium hepaticum among dogs and cats, pets or stray animals, captured by the Zoonosis Control Center (CCZ) in Lages, Santa Catarina, Brazil. Fecal samples from 108 pet dogs and eight pet cats, and from 357 stray dogs and 97 stray cats, captured by CCZ, were analyzed within the period from July 2010 to November 2012. Coproparasitological exams were performed by techniques of sedimentation, centrifuge-flotation, and simple flotation. Among 465 fecal samples from dogs and 105 from cats, the overall spurious infections for C. hepaticum eggs were 1.05%. For dogs, this positivity was 0.43% and for cats it was 3.81%. The two positive dogs were stray and out of the four cats, three were stray and one was a pet. Although the occurrence of C. hepaticum eggs was low, these data reveal the existence of infected rodents, especially in public places, since, out of the six infected animals, five (83.33%) were stray. These results are discussed and analyzed with an emphasis on the risk to public health.

Treatment of Schistosoma mansoni with miltefosine in vitro enhances serological recognition of defined worm surface antigens

“…Miltefosine (hexa-decyl-phosphocholine) was purchased from Calbiochem (Cambridge, UK) and praziquantel (PZQ) from Merck Ltd (UK). For in vitro treatment, drugs were dissolved in a minimal volume of 0.5% dimethyl sulfoxide (DMSO) diluted in Roswell Park Memorial Institute (RPMI) 1640 medium (Invitrogen, UK) to generate a stock solution of 5 mg/ml [ 16 , 38 , 39 ].…”

“…Worms were divided into three groups, each being tested in triplicate, as follows: Group I, untreated worms in a medium containing 0.5% DMSO (vehicle) were used as negative controls (n = 10); Group II, worms treated with PZQ; Group III, worms treated with miltefosine. Groups II and III were further subdivided into 5 and 4 subgroups, respectively (n = 10 for each) to test different concentrations of the PZQ: 2, 4, 6, 8 and 10 μg/ml [ 38 ] and the miltefosine: 5, 10, 20 and 40 μg/ml [ 16 ]. All groups were incubated at 37°C in 5% CO 2 incubator for 96 hours.…”

Severe granulomatous hepatitis caused by Capillaria hepatica in a puppy

“…In addition to the eggs, adult female C. hepatica was observed in the liver of the puppy. As far as we know, in canine cases reported by different authors (6,7,9) only the eggs of the parasite were detected and no adult parasites were found. The presence of eggs in the absence of adult parasites in other studies suggests that adult parasites were degenerated.…”

An initial coprological survey of parasitic fauna in the wild Amur leopard (Panthera pardus orientalis)

“…2015; Quadros et al . 2016). E. aerophilus is a globally distributed parasite found among various wild carnivorous mammals that resides in the epithelium of the host's trachea, bronchi, and bronchioles of the lungs.…”