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Bonesso, Mariana Fávero; Marques, Sílvio Alencar; Cunha, Matheus Luís Oliveira

doi:10.1590/s1678-91992011000400004

Cited by 5 publications

(2 citation statements)

References 56 publications

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“…ETA is an SGB prophage-encoded virulence factor and was present among all MRSA strains harboring this prophage type. SAK is a 16-kDa prophage-encoded protein, which acts as a fibrin-specific activator of human plasminogen produced by certain S. aureus strains, which indicates the proteolytic activity of MRSA strains (33). We found that 93% of MRSA strains isolated from different clinical samples such as urine, wound, sputum and cerebrospinal fluid (CSF) were positive for sak gene, which is higher than the previous report from Iran (18).…”

Section: Discussionmentioning

confidence: 62%

Characterization of Virulence Factors and Prophage Profiles of Methicillin-Resistant Staphylococcus aureus Strains Isolated from a Referral Hospital in Tehran, Iran

Rahimi

Shokoohizadeh

2018

Arch Clin Infect Dis

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Background: Methicillin-resistant Staphylococcus aureus (MRSA) has been considered as an important pathogen with a variety of virulence factors in communities and hospitals worldwide. Objectives: In this study, we focused on the detection of different virulence factors and enterotoxin genes of MRSA strains isolated from a referral hospital in Tehran, Iran. Moreover, the presence of different prophage types was studied. Methods: A total of 491 MRSA strains were isolated during three years from a referral hospital in Tehran. The staphylococcal enterotoxin (sea-seq) and pvl, hlb, sak, eta and tst genes were detected. A multiplex-polymerase chain reaction (PCR) assay was used for prophage typing of MRSA isolates. Results: Totally, 11 enterotoxin and 5 virulence factor genes were detected in MRSA strains. The sea, sek, seq, and hlb genes were present in all the MRSA and other enterotoxin genes. sel, seg, sem, sei, sen, seo, sec and sep were detected in 32.8%, 20.3%, 12.6%, 8.3%, 4.1%, 2.6%, 1.6% and 0.4% of the strains, respectively. A total of 93%, 81%, 15.9% and 5.7% of the strains harbored the sak, eta, tst and pvl genes, respectively. SGF, SGFa and SGFb proghage type genes were detected in 100% of the MRSA strains, and four different prophage patterns were identified among the strains. Conclusions:The presence of different prophage-encoded virulence factors among MRSA strains enable MRSA to produce a broad range of diseases, indicating MRSA strains as a potential threat to patients' health.

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Section: Discussionmentioning

confidence: 62%

Characterization of Virulence Factors and Prophage Profiles of Methicillin-Resistant Staphylococcus aureus Strains Isolated from a Referral Hospital in Tehran, Iran

Rahimi

Shokoohizadeh

2018

Arch Clin Infect Dis

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“…Nosocomial strains of MRSA are typically multi-resistant and are associated with SCCmec type I, II or III, which comprise genes that code for resistance to various classes of antibiotics [ 29 , 30 , 31 , 32 , 33 ]. These strains are usually treated with a combination of two oral antimicrobials [ 34 ]; however, the escalation of divergent resistant phenotypes has created a need to develop new therapeutic alternatives, preferentially with lower hazardous side effects.…”

Section: Discussionmentioning

confidence: 99%

Epigenetic and Drug Response Modulation of Epigalocaten-In-3-Gallate in Staphylococcus aureus with Divergent Resistance Phenotypes

et al. 2023

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Healthcare-associated methicillin-resistant Staphylococcus aureus infections represent extremely high morbidity and mortality rates worldwide. We aimed to assess the antimicrobial potential and synergistic effect between Epigalocatenin-3-gallate (EGCG) and different antibiotics in S. aureus strains with divergent resistance phenotypes. EGCG exposure effects in epigenetic and drug resistance key modulators were also evaluated. S. aureus strains (n = 32) were isolated from infected patients in a Lisbon hospital. The identification of the S. aureus resistance phenotype was performed through automatized methods. The antibiotic synergistic assay was performed through disk diffusion according to EUCAST guidelines with co-exposure to EGCG (250, 100, 50 and 25 µg/mL). The bacteria’s molecular profile was assessed through FTIR spectroscopy. The transcriptional expression of OrfX, SpdC and WalKR was performed by using qRT-PCR. FTIR-spectroscopy analysis enabled the clear discrimination of MRSA/MSSA strains and the EGCG exposure effect in the bacteria’s molecular profiles. Divergent resistant phenotypes were associated with divergent transcriptional expression of the epigenetic modulator OrfX, particularly in MRSA strains, as well as the key drug response modulators SpdC and WalKR. These results clearly demonstrate that EGCG exposure alters the expression patterns of key epigenetic and drug response genes with associated divergent-resistant profiles, which supports its potential for antimicrobial treatment and/or therapeutic adjuvant against antibiotic-resistant microorganisms.

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Clinico-microbiological study of community acquired MRSA from skin and soft tissue infections and its antibiogram in a tertiary care hospital in Karnataka

Thilagavathy

Chavan

2020

IJMR

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Methicillin resistant staphylococcus aureus (MRSA) has always been a worldwide problem, although its prevalence varies considerably among countries. The epidemiology of MRSA has changed over the years and infections are no longer confined to the hospital setting, but appear in healthy community dwelling individuals with no risk factors. From skin and soft tissue infection, common organism isolated is Community associated MRSA (CA-MRSA). Hence the study was done to know the prevalence of MRSA among community associated skin and soft tissue infections in Basaveshwar teaching and general hospital, attached to M.R. Medical College, Gulbarga. Standard techniques were used to isolate Staphylococcus aureus from clinical specimens. Cefoxitin disc diffusion was used to find MRSA. Antibiogram of MRSA was detected by Kirby Bauer disc diffusion method. Inducible Clindamycin resistance was done by Double Disc Diffusion method (D test). From over 200 cases of CA-MRSA, total of (75.5%) staphylococcus aureus was detected. Out of them, CA-MRSA was 27 (17.9%). These showed high sensitivity to Vancomycin (100%), Linezolid (96.2%),Cindamycin (92.59%), moderately susceptible to TMP-SMX(85.1%), Rifampicin (88.1%), Tetracycline(81.4%),Gentamicin (70.3%), ciprofloxacin(62.9%) and a low susceptibility to Erythromycin (48.14%). 14.8% of CA-MRSA strains were D test positive (inducible MLS positive) and 29.63% were D test negative (MS phenotype). 7.4% of CA-MRSA were positive for constitutive MLS resistance. There is a need for judicious selection of antimicrobial agents, as their indiscriminate use can exert pressure in selecting MRSA and other multi-drug resistant organisms. Further spread of community acquired infections can be done by effective infection control programs.

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Cited by 5 publications

References 56 publications

Characterization of Virulence Factors and Prophage Profiles of Methicillin-Resistant Staphylococcus aureus Strains Isolated from a Referral Hospital in Tehran, Iran

Characterization of Virulence Factors and Prophage Profiles of Methicillin-Resistant Staphylococcus aureus Strains Isolated from a Referral Hospital in Tehran, Iran

Epigenetic and Drug Response Modulation of Epigalocaten-In-3-Gallate in Staphylococcus aureus with Divergent Resistance Phenotypes

Clinico-microbiological study of community acquired MRSA from skin and soft tissue infections and its antibiogram in a tertiary care hospital in Karnataka

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