Chemical composition and antibacterial activity of Brazilian propolis essential oil

Abstract

ABSTRACT:The present study aimed at investigating the chemical composition of essential oil extracted from Brazilian propolis and the susceptibility of Staphylococcus aureus, Staphylococcus epidermides, Streptococcus pyogenes and Escherichia coli to this substance. The essential oil was obtained by steam distillation of propolis and examined by gas chromatography/mass spectrometry (GC/MS). In addition, the agar diffusion method using filter paper disks was employed. Antibacterial activity was measured as equivalent diameters of inhibition zones (in millimeters) after incubation at 37C for 24 hours. From the 26 identified constituents, β-caryophyllene (12.7%), acetophenone (12.3%) and β-farnesene (9.2%) were found to be major components. New components, namely linalool, methyl hydrocinnamate, ethyl hydrocinnamate, α-ylangene, γ-elemene and valencene, are reported for the first time to be present in propolis essential oil. This oil also exhibited antibacterial activity.

A comparative study of volatile components of propolis (bee glue) collected from Haramaya University and Assela Beekeeping Centers, Ethiopia

“…The Iranian propolis essential oil (Lalehzar origin) was found to be rich in α-pinene (43.9%), 1,8-cineole (11.1%), camphene (8.6%), β-myrcene (3.9%), broneol (3.4%), camphor (2.4%) and verbenol (2.3%) where as the Propolis essential oil of Kerman origin contains α-pinene (46.1%), 1,8-cineole (11.1%), camphene (9.6%), camphor (5.3%), sabinene (4.6%), β-myrcene (3.9%), broneol (3.4%), bornyl acetates (2.8%), verbenone (2.3%) and linalool (2.1%) [26]. Moreover, the most abundant components in Brazilian propolis essential oil were β-caryophyllene (12.7%), acetophenone (12.3%), farnesene (9.2%) and linalool (6.47%), followed by γ-elemene (6.25%), γ-cadinene (5.86%) and γ-muurolene (3.61%) [15].…”

“…Knowledge of volatile components of propolis is so important as its oil exhibited antibacterial activity against Staphylococcus aureus, Staphylococcus epidermides, Streptococcus pyogenes and Escherichia coli and also used for special flavor and cosmetics [15].…”

Chemical composition and antimicrobial activities of leaves of sweet basil (Ocimum basilicum L.) herb

“…9 Knowledge of volatile components of Sweet basil is as important as its oil exhibited antibacterial activity against Escherichia coli, Staphylococcus aureus, Staphylococcus epidermides, Streptococcus pyogenes as well as antifungal activities against Aspargillus niger and Rhizocaticol bataticola and also used for special flavor and cosmetics. 10 So, there has been a research gap as there is no any work especially about isolation and characterization of the pure components of the crude extract from the leaves and antimicrobial activities of this herb in Ethiopia. So, since such medicinal herbs are widely distributed in different regions of Ethiopia and are traditionally used in the treatment of gonorrhea, dysentery, chronic diarrhea, wound healing, pimples, skin infections, and against different varieties of diseases, it was found to conduct the herb for chemical and antimicrobial investigation of the plant leaves.…”

Composition of the volatile fraction of a sample of Brazilian green propolic and its phytotoxic activity

“…Ioshida et al detected neither caryophyllene nor nerolidol in the volatiles from Brazilian propolis; instead, other sesquiterpenes were reported, in addition to monoterpenes . Oliveira and co‐workers found a distinct composition, based mainly on caryophyllene, acetophenone and β ‐farnesene, in addition to α ‐terpineol, δ ‐cadinene, spathulenol, α ‐ylangene, γ ‐elemene and valencene …”