Histopathological evaluation in experimental envenomation of dogs with Crotalus durissus terrificus venom

Abstract

The present work evaluated histopathological aspects in experimental envenomation of dogs with Crotalus durissus terrificus venom. Twenty-eight mixed breed adult dogs were divided into three groups of seven animals each: Group I - only venom; Group II - venom + 50ml antiophidic serum + fluid therapy; Group III - venom + 50ml antiophidic serum + fluid therapy + urine alkalization. Lyophilized venom of Crotalus durissus terrificus was reconstituted in saline solution and inoculated subcutaneously at the dose of 1mg/kg body weight. Three animals of each group were subjected to euthanasia, and their muscular tissue, brain, spleen, kidneys, heart, lungs, stomach, small and large intestines, and popliteal lymph node fragments were collected for histopathological evaluation. There was myonecrosis in the inoculated limb, renal tubular degeneration, lymphoid hyperplasia of spleen, and unspecific reactive hepatitis. These results show the antigenicity and action of the venom on the immune system

Effects of Mikania glomerata Leaf Extract on Experimental Bothropoides jararaca Envenomation in Wistar Rats

“…Ophidic accidents are a problem in many parts of the world, especially in tropical and subtropical areas, with most cases caused by the Bothrops genus [1,[15][16][17].…”

Fractal Dimension in Liver Histological Findings of Wistar Rats Experimentally Intoxicated with Venom of Crotalus durissus terrificus

“…In this study, the histological changes observed together with the increase in DF values, it is possible to report that the crotalic venom causes an injury of importance and severity in the liver of experimentally intoxicated animals with a dose of 1 mg/kg. Other authors have also reported moderate to severe liver damage in humans [2,3], mouse [13] and dog [30] as a result of the accident caused by the venom of Crotalus durissus.…”

“…In current studies, on the action of venom of ophidians in various tissues and organs histological analysis is performed qualitatively through tissue characteristics, cell morphology, size, nucleus position, presence of inflammatory infiltrate, and other findings [28][29][30]. However it is known that this type of analysis is dependent on the analyzer, which leads to intra and interobserver variations [21].…”

Efectos bioquímicos e histopatológicos en ratas inducidos por el veneno de Crotalus durissus cumanensis del municipio Falcón, estado Falcón, Venezuela

“…También hay evidencia de daño del tejido renal con congestión cortical a partir de la tercera hora posinyección de veneno hasta llegar a la congestión de las zonas yuxtaglomerulares con escasa cantidad de cilindros hialinos (29) y degeneración tubular renal (52). Por otra parte, el daño renal es una causa importante de muerte en los pacientes que sobreviven a los efectos iniciales de los severos envenenamientos crotálicos.…”

“…Por el contrario, los resultados no coinciden con lo reportado por Sangiorgio et al(52), quienes señalan que el veneno de C. durissus terrificus afecta el músculo esquelético, riñón, bazo e hígado, pero no afecta el músculo cardiaco. De igual manera, Posner et al(53) indican que al parecer el tejido cardiaco es relativamente resistente al efecto del veneno de Crotalus atrox.…”