Immunological assessment of mice hyperimmunized with native and Cobalt-60-irradiated Bothrops venoms

Ferreira, Rafaela Scalco; Nascimento, N.; Martı́nez, Javier; Alves, Janaina Baptista; Meira, Domingos Alves; Barraviera, Benedito

doi:10.1590/s1678-91992005000400006

Cited by 3 publications

(2 citation statements)

References 36 publications

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“…Gamma radiation has been efficient in attenuating ophidic venoms and in decreasing toxicity without altering immunogenicity, and the addition of substances to the venom was not necessary (1,2,8,10,16,20,21,32).…”

Section: Introductionmentioning

confidence: 99%

Laboratory evaluation of young ovines inoculated with natural or 60co-irradiated Crotalus durissus terrificus venom during hyperimmunization process

Ferreira

Nascimento

Couto

et al. 2006

J. Venom. Anim. Toxins incl. Trop. Dis

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Laboratory profile of young ovines was studied in order to evaluate and compare their antiserum production from natural and Cobalt-60 irradiated Crotalus durissus terrificus (C.d.t.) venoms. The parameters analyzed included complete blood count, and urea, creatinine, aspartate aminotransferase, total proteins, albumin and globulin serum measurements. Three groups of six animals each were used. Group 1 (G1) received natural C.d.t. venom; Group 2 (G2) received irradiated C.d.t. venom; and Group 3 (G3) was used as control and did not receive venom, only adjuvants, using seven venom inoculations. During the experimental period, animals were fortnightly weighed. According to clinical and weight evaluation, sheep in post-weaning phase showed no changes in their physiological profiles but had excellent weight gain. The parameters analyzed were not statistically different (p<5%) among the groups tested. The hyperimmunization process was successfully accomplished with the production of specific antibodies against Crotalus durissus terrificus venom. Results bring a new possibility of utilizing ovines in the commercial production of anticrotalic serum, which may be used to treat human and animal envenomation. Its production cost may be reduced by subsequent use of hyperimmunized sheep for human consumption

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Section: Introductionmentioning

confidence: 99%

Laboratory evaluation of young ovines inoculated with natural or 60co-irradiated Crotalus durissus terrificus venom during hyperimmunization process

Ferreira

Nascimento

Couto

et al. 2006

J. Venom. Anim. Toxins incl. Trop. Dis

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“…Gamma radiation from 60 Co has been used as an attenuating agent of the venoms toxic properties (14,37,38). Irradiation of biological materials from a 60 Co source is the most used method because it does not require heating the venom which is susceptible to high temperatures (18).…”

Section: Introductionmentioning

confidence: 99%

Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venom

Oliveira¹,

Sakate²,

Madruga³

et al. 2007

J. Venom. Anim. Toxins incl. Trop. Dis

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Venoms from snakes of the Bothrops genus are proteolytic, coagulant, hemorrhagic and nephrotoxic, causing edema, necrosis, hemorrhage and intense pain at the bite site, besides systemic alterations. Many adjuvants have been added to the venom used in the sensitization of antiserum-producer animals to increase antigenic induction and reduce the envenomation pathological effects. Gamma radiation from 60Co has been used as an attenuating agent of the venoms toxic properties. The main objective was to study, comparatively, clinical and laboratory aspects of goats inoculated with bothropic (Bothrops jararaca) venom, natural and irradiated from a 60Co source. Twelve goats were divided into two groups of six animals: GINV, inoculated with 0.5mg/kg of natural venom; and GIIV, inoculated with 0.5mg/kg of irradiated venom. Blood samples were collected immediately before and one, two, seven, and thirty days after venom injection. Local lesions were daily evaluated. The following exams were carried out: blood tests; biochemical tests of urea, creatinine, creatine kinase (CK), aspartate amino-transferase (AST) and alanine amino-transferase (ALT); clotting time; platelets count; and total serum immunoglobulin measurement. In the conditions of the present experiment, irradiated venom was less aggressive and more immunogenic than natural venom

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Resposta imune e capacidade de neutralização de anticorpos produzidos em ovinos jovens imunizados com veneno de Crotalus durissus terrificus nativo e irradiado com Cobalto 60

Ferrreira

Nascimento

Couto

et al. 2009

Braz. J. Vet. Res. Anim. Sci.

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A técnica de Elisa foi utilizada para avaliar e comparar a resposta imune humoral de ovinos jovens para a produção de soro anticrotálico. Durante o processo de soroprodução, foi realizada a avaliação clínica dos animais. A capacidade de neutralização do soro produzido a partir de veneno de serpente Crotalus durissus terrificus, nativo (VN) e irradiado (VIr) com Cobalto-60 foi verificada por meio de desafios in vitro. Um grupo de seis animais recebeu veneno nativo, o segundo grupo recebeu veneno irradiado e o terceiro grupo foi o controle. Os animais receberam seis imunizações durante 84 dias com intervalo de 14 dias. Houve diferença significativa (p<5%) no teste de ELISA do perfil de anticorpos produzidos pelos grupos experimentais (VN < VIr). O grupo imunizado com veneno irradiado apresentou perfil de anticorpos maior que o grupo imunizado com veneno nativo. A capacidade de neutralização do soro produzido a partir do VIr foi cinco vezes maior quando comparado ao soro produzido com VN. Estes resultados justificam o uso da radiação gama na destoxicação do veneno de Crotalus durissus terrificus como alternativa na produção de antiveneno.

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Immunological assessment of mice hyperimmunized with native and Cobalt-60-irradiated Bothrops venoms

Cited by 3 publications

References 36 publications

Laboratory evaluation of young ovines inoculated with natural or 60co-irradiated Crotalus durissus terrificus venom during hyperimmunization process

Laboratory evaluation of young ovines inoculated with natural or 60co-irradiated Crotalus durissus terrificus venom during hyperimmunization process

Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venom

Resposta imune e capacidade de neutralização de anticorpos produzidos em ovinos jovens imunizados com veneno de Crotalus durissus terrificus nativo e irradiado com Cobalto 60

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