Evaluation of tissue reaction, cell viability and cytokine production induced by Sealapex Plus

Abstract

ObjectiveThe aim of this study was to investigate the effects of mineral trioxide aggregate (MTA), Sealapex, and a combination of Sealapex and MTA (Sealapex Plus) on the reaction of subcutaneous connective tissue of rats, and on cell viability and cytokine production in mouse fibroblasts. Material and MethodsThe tissue reaction was carried out with dentin tubes containing the materials implanted in the dorsal connective tissue of rats. The histological analysis was performed after 7 and 30 days. Millipore culture plate inserts with polyethylene tubes filled with materials were placed into 24-well cell culture plates with mouse fibroblasts to evaluate the cell viability by MTT assay. ELISA assays were also performed after 24 h of exposure of the mouse fibroblasts to set material disks. ResultsHistopathologic examination showed Von Kossa-positive granules that were birefringent to polarized light for all the studied materials at the tube openings. No material inhibited the cell viability in the in vitro test. It was detected IL-6 production in all root-end filling materials. MTA and Sealapex Plus induced a slight raise of mean levels of IL-1ß. ConclusionsThe results suggest that Sealapex Plus is biocompatible and stimulates the mineralization of the tissue.

Evaluation of photodynamic therapy on fibroblast viability and cytokine production

“…Saline solution and PDT were capable to maintain the cell viability similar to that observed for control during all periods evaluated. These results are similar to previous studies that have showed that PDT did not affect host cell viability from gingival fibroblasts, osteoblasts, and human periodontal ligament cells [22][23][24][25][26][27][28][29][30][31]. On the other hand, disagree with others that have showed that PDT promoting reduction in fibroblast, macrophage and human head [32,33].…”

“…The cell viability was determined in this study by 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay (MTT) based on the ability of mitochondrial dehydrogenase enzymes in living cells to convert the yellow water-soluble tetrazolium salt MTT into dark blue formazan crystals, a simple method that showed advantages as rapidness and precision [21,22] and the substrate used in the assay does not interfere with measurement end [23]. Complementary, ELISA assay was used for the measured the cytokines secreted.…”

“…At the experimental times, stock MTT solution (40 L per 360 L medium) was added to all wells of an assay, and plates were incubated at 37 • C for 3 h. The medium was then removed by the inversion of the plate and 200 mL of isopropilic alcohol was added to the wells and mixed during 20 minutes in order to dissolve the dark blue crystals. The blue solution was transferred to a 96-well plate, and the absorbance was read in the microplate reader using 570 nm wavelength [23]. The treatments were classified according to the cytotoxic effect of: 0-non-cytotoxic (less inhibition than 25%); 1-slightly cytotoxic (inhibition between 25% and 50%); 2-moderately cytotoxic (inhibition between 50% and 75%) and 3-strongly cytotoxic (greater than 75% inhibition).…”

In Vitro and In Vivo Toxicity Evaluation of Colloidal Silver Nanoparticles Used in Endodontic Treatments

“…To further clarify the biocompatibility of silver nanoparticles, a model of implantation in the subcutaneous tissue of rats was also used, which is 1 of the most appropriate tests to determine the local effects of compounds (8,33). In the present study, we have analyzed the tissue reaction to SNA and SNP at 1.0 mg/mL.…”

Avaliação do perfil de citocinas no tecido subcutâneo de camundongos na presença de cimento endodôntico

“…Outro aspecto que vem sendo estudado é a capacidade dos cimentos endodônticos interferirem na resposta imune (Bernath e Szabo, 2003;De Oliveira Mendes, Ribeiro Sobrinho et al, 2003;Gomes-Filho, Watanabe et al, 2009;Gomes-Filho, Gomes et al, 2011). Associando-se a inflamação já existente fruto do processo patológico à possível irritação ocasionada pelos materiais obturadores, a resposta inflamatória poderá ser persistente mesmo após a obturação (Perassi, Filho et al, 2004).…”