Histologic grading and nucleolar organizer regions in oral squamous cell carcinomas

Hanemann, João Adolfo Costa; Miyazawa, Marta; Souza, Mireile São Geraldo dos Santos

doi:10.1590/s1678-77572011000300018

Cited by 14 publications

(16 citation statements)

References 24 publications

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“…In the present study, the frequency of small, large and total AgNORs increased with malignant progression from normal mucosa to squamous cell carcinomas of the oral cavity ((P<0.05); Table I), in agreement with earlier report [17][18][19] . This clearly indicates that the total AgNOR counts increase with the changes occurring in the normal epithelium towards a dysplastic change and its malignant transformation.…”

Section: Discussionsupporting

confidence: 93%

“…Even though the reason for the greater number of AgNORs in the nuclei of malignant lesions is uncertain, it may represent an escalation in ploidy 19 , increased gene amplification or a rise in chromosomal segregation with more cells being in the S phase of their cycle 21 . In agreement with Derenzini et al 14 , we also found an inverse correlation between malignant change and the size of AgNORs.…”

Section: Discussionmentioning

confidence: 99%

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Quantitative estimation of AgNORs in normal, dysplastic and malignant oral mucosa

Chowdhry¹,

Deshmukh²,

Shukla³

et al. 2014

BIOMED PAP

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Aims. Silver stainable nucleolar organizer regions (AgNORs) have received a great deal of attention recently as their frequency within the nuclei is significantly higher in malignant cells than in normal, reactive or benign neoplastic cells. The objective of this study was to carry out a quantitative assessment of large and small AgNORs in oral normal mucosa, precancerous lesions and infiltrating squamous cell carcinomas. Methods. The study comprised 110 formalin-fixed, paraffin-embedded oral mucosal biopsies consisting of 30 oral dysplasia, 60 oral squamous cell carcinomas and 20 normal oral mucosa. AgNORs were counted in each nucleus, categorized as small, large and total number of AgNORs in each cell and their means were calculated. Results. The mean value of small AgNORs, large AgNORs and total AgNORs increased gradually from normal mucosa to dysplastic lesions to squamous cell carcinomas. The study clearly indicates that in oral squamous cell carcinomas, AgNORs diminish in size as they increase in number. Further, AgNOR counts increase as the degree of malignant potential of the cell increases. Conclusions. By combining both the enumeration of AgNORs and their size, good distinction can be made between normal, dysplastic and infiltrating squamous cell carcinomas. This could help in the early diagnosis and prognosis of dysplastic mucosal lesions and their malignant transformation.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Quantitative estimation of AgNORs in normal, dysplastic and malignant oral mucosa

Chowdhry¹,

Deshmukh²,

Shukla³

et al. 2014

BIOMED PAP

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“…Burakselvi et al (2015) also found a significant correlation between age and AgNORs area in buccal epithelial cells of healthy individuals, which supports our study (1). Many studies have been done on the basis of number of AgNOR but according to Joao et al(2011) to avoid different factors which affect the numeric factors of AgNOR, morphometric analysis should be done to avoid the mistakes produced by union of particles 7 .…”

supporting

confidence: 86%

Age and AgNor- A Morphometric study

Kaur¹,

Naik²,

Jindal³

2020

SPR

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To investigate and correlate total AgNOR area/Total nucleus area (TAA/TNA ) values in buccal epithelial cells of healthy individuals in different age groups. Material and Methodology:- In present study 50 healthy individuals are included with age ranging 10 to 60 years. These are divided into 5 groups.Group1-10-20year, Group2-20- 30year, Group3-30-40year, Group4-40-50year, Group5-50-60year. Oral epithelial cells collected with the help of cyto brush from buccal mucosa. Smears were prepared on clean glass slides and fixed with 95% alcohol. Fixed slides Smears were stained with AgNOR stain. Results: Statistically significant correlations were found between mean TAA/TNA values and age p < 0.001 for linear and p < 0.0001 for polynominal regression, and between AgNOR number and age, p < 0.001 for linear. Conclusion: There is a significant correlation between age and AgNOR amount (ribosome biosynthesis rate) in buccal epithelial cells of healthy individuals. AgNORs in buccal epithelial cells may be used for detection of age.

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“…Several studies have shown that AgNOR counts can be used to differentiate between benign and malignant lesions (7)(8)(9)(10). This technique plays a major role in estimating the proliferation potential of neoplasms in the surgical pathological practice (11)(12)(13)(14)(15). However, there are only a few studies of AgNOR counts in odontogenic tumors (16,17).…”

Section: Introductionmentioning

confidence: 99%

Expression of Argyrophilic Nucleolar Organizing Region Counts and Proliferating Cell Nuclear Antigen in Epithelial Odontogenic Tumors

Mohamed

Darwish

El‐Sheikh

et al. 2019

Alexandria Dental Journal

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INTRODUCTION:Odontogenic tumors are diverse groups of lesions derived from odontogenic tissues. Clinical behavior of these lesions varies widely, ranging from benign to malignant with differences in recurrence rate and growth pattern. A better understanding of the underlying molecular mechanisms will help to predict the course of odontogenic tumors and lead to the development of new therapeutic applications. Nucleolar organizer regions (NORs) are loops of DNA that codify the ribosomal RNA and are considered important for protein synthesis. The NORs stained histochemicaly by silver are named argyrophilic nucleolar organizer regions (AgNOR). Quantification of AgNOR represents a valuable parameter of cell kinetics and therefore cell proliferation. The number of AgNOR sites in malignant cells is significantly greater than those in their normal or benign counterparts. Proliferating cell nuclear antigen (PCNA) is a useful marker of cellular proliferation and DNA replication. Malignant tissue is characterized by an uncoordinated proliferating cell nuclear antigen (PCNA). OBJECTIVES: To evaluate the cell proliferation rate using the expression of Argyrophilic Organizing Region counts and Proliferating Cell Nuclear Antigen in epithelial odontogenic tumors and to assess the usefulness of AgNOR and PCNA as markers in malignant potential of epithelial odontogenic tumors. MATERIALS AND METHODS: This study was done on 45 surgical specimens of epithelial odontogenic tumors and one control specimen from normal tooth germ.The tissue biopsies were processed and paraffin sections were prepared. Hematoxylin and eosin-stained sections were examined for diagnosis. Other sections were subjected to AgNORhistochemical technique and immunohistochemical staining for PCNA. Immunohistochemical staining was performed using a Labeled Strept-Avidin Biotin complex method (LSAB). RESULTS: The mean AgNOR count of tooth germ was 1.21 and the benign odontogenic tumors was 1.862.The highest AgNOR count was recorded in Ameloblastic carcinoma and lowest was seen in adenomatoid odontogenic tumor. Statistically significant difference in AgNOR counts of ameloblastoma and adenomatoid odontogenic tumor, ameloblastoma and calcifying epithelial odontogenic tumor, benign odontogenic tumors and ameloblastic carcinoma were seen. AgNORs in ameloblastic carcinoma were more in number and more widely distributed.The highest mean value of PCNA-positive cell percentage was demonstrated in ameloblastic carcinoma followed by ameloblastoma. A significant difference was noted when the PCNA value of ameloblastoma was compared with that of ameloblastic carcinoma. CONCLUSIONS: AgNOR technique may be considered a good indicator of cell proliferation in epithelial odontogenic tumors.The PCNA expression, along with the clinical features, can predict the aggressiveness, chances of recurrence and malignant potential of these tumors.

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Histologic grading and nucleolar organizer regions in oral squamous cell carcinomas

Cited by 14 publications

References 24 publications

Quantitative estimation of AgNORs in normal, dysplastic and malignant oral mucosa

Quantitative estimation of AgNORs in normal, dysplastic and malignant oral mucosa

Age and AgNor- A Morphometric study

Expression of Argyrophilic Nucleolar Organizing Region Counts and Proliferating Cell Nuclear Antigen in Epithelial Odontogenic Tumors

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