Detection of selected bacterial species in intraoral sites of patients with chronic periodontitis using multiplex polymerase chain reaction

BackgroundEnterococci are increasingly associated with opportunistic infections in Humans but the role of the oral cavity as a reservoir for this species is unclear. This study aimed to explore the carriage rate of Enterococci in the oral cavity of Tunisian children and their antimicrobial susceptibility to a broad range of antibiotics together with their adherence ability to abiotic and biotic surfaces.ResultsIn this study, 17 E. faecalis (27.5%) and 4 E. faecium (6.5%) were detected. The identified strains showed resistance to commonly used antibiotics. Among the 17 isolated E. faecalis, 12 strains (71%) were slime producers and 5 strains were non-producers. Among the 4 E. faecium, 2 strains were slime producers. All the tested strains were able to adhere to at least one of the two tested cell lines. Our result showed that 11 E. faecalis and 2 E. faecium strains adhered strongly to Hep-2 as well as to A549 cells.ConclusionsDrugs resistance and strong biofilm production abilities together with a high phenotypic adhesion to host cells are important equipment in E. faecalis and E. faecium which lead to their oral cavity colonization and focal infections.

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“…This result was contradictory with a recent study reported a low prevalence rate of E. faecalis (3.5% to 13.5%) in intraoral sites [26]. …”

Section: Discussioncontrasting

confidence: 70%

Antibiotic resistance and adhesion properties of oral Enterococci associated to dental caries

Kouidhi¹,

Zmantar²,

Mahdouani

et al. 2011

BMC Microbiology

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“…Enterococcus faecalis is not readily detected as part of the oral microbiota, and its prevalence in all intraoral microenvironments is reported to range from 3.5% to 13.5%. 18 However, in light of studies using metagenomic approaches, there is moderate evidence to support E. faecalis as a candidate periodontal pathogen, with a potential geographic specificity. 19 Moreover, it is frequently isolated from the root canal system of teeth with failed endodontic treatments.…”

Section: Discussionmentioning

confidence: 99%

Integration of non-oral bacteria into in vitro oral biofilms

Thurnheer

Belibasakis

2014

Virulence

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Abbreviations: CFUs, colony forming units; CLSM, confocal laser scanning microscopy; FISH, fluorescence in situ hybridizationBiofilms are polymicrobial communities that grow on surfaces in nature. Oral bacteria can spontaneously form biofilms on the surface of teeth, which may compromise the health of the teeth, or their surrounding (periodontal) tissues. While the oral bacteria exhibit high tropism for their specialized ecological niche, it is not clear if bacteria that are not part of the normal oral microbiota can efficiently colonize and grow within oral biofilms. By using an in vitro "supragingival" biofilm model of 6 oral species, this study aimed to investigate if 3 individual bacterial species that are not part of the normal oral microbiota (Eschericia coli, Staphylococcus aureus, Enterococcus faecails) and one not previously tested oral species (Aggregatibacter actinomycetemcomitans) can be incorporated into this established supragingival biofilm model. Staphylococcus aureus and A. actinomycetemcomitans were able to grow efficiently in the biofilm, without disrupting the growth of the remaining species. They localized in sparse small aggregates within the biofilm mass. Enterococcus faecalis and E. coli were both able to populate the biofilm at high numbers, and suppressed the growth of A. oris and S. mutants. Enterococcus faecalis was arranged in a chain-like conformation, whereas E. coli was densely and evenly spread throughout the biofilm mass. In conclusion, it is possible for selected species that are not part of the normal oral microbiota to be introduced into an oral biofilm, under the given experimental microenvironmental conditions. Moreover, the equilibrated incorporation of A. actinomycetemcomitans and S. aureus in this oral biofilm model could be a useful tool in the study of aggressive periodontitis and peri-implantitis, in which these organisms are involved, respectively.

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“…Moreover, studies assessing the biofilm composition of root caries have reported more elevated counts of S. mutans when compared with sound root surfaces (10) . Given the high prevalence of root caries in periodontally treated patients with root exposure (18) , it has been hypothesized that high levels of MS may act as a predictor for the onset of carious lesions in this type of patients (11) . Indeed, more root caries have been described during the follow-up of periodontally treated patients with elevated counts of S. mutans than those with lower number of the microorganism (19) .…”

Section: Introductionmentioning

confidence: 99%

Oral colonization by Streptococcus mutans and its association with the severity of periodontal disease in adults

Contardo

Díaz

Lobos

et al. 2011

Rev. Clin. Periodoncia Implantol. Rehabil. Oral

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Background: Streptococcus mutans (S. mutans) is associated with the onset of caries. Since root exposure in patients affected by periodontitis leads to higher caries rates, progressively more severe forms of periodontal disease might associate with elevated counts of S. mutans. Aim: To determine whether increasingly destructive forms of periodontal disease are associated with higher counts of S. mutans in untreated patients. Methods: 206 subjects aged 20-75 were classified into three groups according to the severity of periodontal disease: 1) gingivitis, 2) chronic slight periodontitis and 3) chronic moderate or chronic severe periodontitis. S. mutans counts (cfu/mL) were obtained by direct counting on selective agar plates from saliva samples. A cumulative proportional logistic regression model was adjusted for S. mutans counts. Results: The model failed to show differences by gender, but periodontal diagnosis had a significant effect on S. mutans counts depending on age. While in the group with moderate and severe periodontitis the probability of having high counts of S. mutans significantly increased with age, the probability remained unchanged in individuals with chronic slight periodontitis or gingivitis. Conclusion: High S. mutans levels appear directly co-associated with increased severity of periodontal disease at older ages in untreated patients.

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Detection of selected bacterial species in intraoral sites of patients with chronic periodontitis using multiplex polymerase chain reaction

Cited by 31 publications

References 30 publications

Antibiotic resistance and adhesion properties of oral Enterococci associated to dental caries

Antibiotic resistance and adhesion properties of oral Enterococci associated to dental caries

Integration of non-oral bacteria into in vitro oral biofilms

Oral colonization by Streptococcus mutans and its association with the severity of periodontal disease in adults

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