Fibrin adhesive derived from snake venom in periodontal surgery: histologicalanalysis

Abstract

Background:A new fibrin adhesive made of buffalo plasma-derived fibrinogen and a thrombin-like snake venom enzyme, has been successfully used to immobilize free gingival grafts. This case series histologically compared sutured grafts (control group) with others immobilized by using the fibrin adhesive (experimental group).Case Description:The grafts were placed in the contralateral mandibular bicuspids of 15 patients, so that each subject received one treatment of each type. Five biopsies of each group were collected at 7, 14 and 45 days of healing, which were histologically and morphometrically analyzed as regards the relative volume density of the different connective tissue components.Results:The sites in the control group presented a higher inflammatory cell density at 7 days and a tendency towards a lower collagen density. In the experimental group, the grafts had an appearance of more advanced healing. Tissue maturity characteristics progressed until 14 and 45 days, but no difference between groups could be noted at these times.Conclusions:Within the limits of the present study, it may be suggested that the alternative fibrin adhesive tested could represent an alternative to sutures in gingival grafts procedures.

Unique heterologous fibrin biopolymer with hemostatic, adhesive, sealant, scaffold and drug delivery properties: a systematic review

“…G1 to G6: keratectomized areas with fibrin sealant (collection performed on days 1, 3, 7, 15, 30, 60 after surgery); G7: without sealant.The corneas collected at day 60 presented discrete changes compared to day 30 in postoperative group. Inflammatory cells were rare, and fibrosis was moderate.The fibrin sealant helped in repair of the site, contributing to healing mechanisms and preventing the formation of edema in the wound.Early elimination of fibrin sealant (in three animals) due to the presence of substances in the tear that caused lysis of fibrin. Barbosa et al (2008) [30] To compare and to evaluate histological effects in periodontal surgery, especially in gingival grafts, using the new heterologous fibrin sealant compared with traditional sutures, in humans.Fifteen patients - with surgical procedures in two contralateral areas - were divided into control (only traditional suture technique) and experimental group (fibrin sealant).At day 14, the experimental group had a more mature epithelial tissue than the control group, but both presented epithelial tissue. At day 45, there were no significant difference between the groups, with an increase in collagen fibers.…”

“…They studied 15 non-smoking patients who needed bilateral gingival grafts around the bicuspid mandibular area. They concluded that the HFS is an alternative to the traditional fibrin adhesive and may represent an alternative to sutures in periodontal surgery [29,30]. In addition, Chiquito et al.…”

“…After these trials, several pioneering experimental studies have been carried out. Currently, the new sealant is being used in several clinical and biotechnological practices, such as in dental surgeries [8,29,30], recovery of injured nervous [31-33], bone repair [34-36], and treatment of chronic venous ulcers [37,38,39].…”

Fibrin Biopolymer Incorporated with Antimicrobial Agents: A Proposal for Coating Denture Bases

“…More recently, a new heterologous fibrin sealant composed of fibrinogen-rich cryoprecipitate, extracted from Bubalus bubalis buffalo blood, and a thrombin-like enzyme, purified from the venom of Crotalus durissus terrificus, has been considered as a promising material in medicine and dentistry [ 32 , 33 , 34 , 35 , 36 ]. This biopolymer has biocompatibility (nontoxic), low-cost to produce in addition to hemostatic, sealant, adhesive, scaffold, and drug delivery properties [ 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 ]. In the presence of calcium, the thrombin-like enzyme acts on fibrinogen molecules, turning them into fibrin monomers, resulting in a stable clot [ 33 , 34 , 35 ].…”

“…This biopolymer has biocompatibility (nontoxic), low-cost to produce in addition to hemostatic, sealant, adhesive, scaffold, and drug delivery properties [ 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 ]. In the presence of calcium, the thrombin-like enzyme acts on fibrinogen molecules, turning them into fibrin monomers, resulting in a stable clot [ 33 , 34 , 35 ]. In dentistry, Barbosa et al [ 35 , 40 ] and Chiquito et al [ 41 ] reported that using FB (fibrin biopolymer) to immobilize free gingival grafts is as effective as conventional sutures.…”