Bone regeneration after demineralized bone matrix and castor oil (Ricinus communis) polyurethane implantation

Abstract

Innocuous biocompatible materials have been searched to repair or reconstruct bone defects. Their goal is to restore the function of live or dead tissues. This study compared connective tissue and bone reaction when exposed to demineralized bovine bone matrix and a polyurethane resin derived from castor bean (Ricinus communis). Forty-five rats were assigned to 3 groups of 15 animals (control, bovine bone and polyurethane). A cylindrical defect was created on mandible base and filled with bovine bone matrix and the polyurethane. Control group received no treatment. Analyses were performed after 15, 45 and 60 days (5 animals each). Histological analysis revealed connective tissue tolerance to bovine bone with local inflammatory response similar to that of the control group. After 15 days, all groups demonstrated similar outcomes, with mild inflammatory reaction, probably due to the surgical procedure rather than to the material. In the polymer group, after 60 days, scarce multinucleated cells could still be observed. In general, all groups showed good stability and osteogenic connective tissue with blood vessels into the surgical area. The results suggest biocompatibility of both materials, seen by their integration into rat mandible. Moreover, the polyurethane seems to be an alternative in bone reconstruction and it is an inexhaustible source of biomaterial.

Polyurethane and PTFE membranes for guided bone regeneration: Histopathological and ultrastructural evaluation

“…Foreign-body giant cells did accumulate around the polymer and in its pores, suggesting the degradation is facilitated by hydrolysis as well as by giant cells. More important, subcutaneous implants of the polymer allowed infiltration of vascular and connective tissue, suggesting the free flow of fluids and nutrients in the implants (7,14). The osteogenic tissue presence in the polymer surface and the membrane incorporation in the newly formed bone tissue were related (4,17).…”

“…The castor oil polyurethanes present an excellent biological compatibility (12)(13)(14). The observations on the inflammatory infiltrate in the samples treated with polyurethane barrier, showed an initial inflammatory reaction which decreased along the time (4), and the presence of inflammatory multinuclear giant cells (IMGC) (7). Although the tissue reaction varied from mild to absent, the polymer acts as a strange body, promoting an inflammatory infiltrate of mononuclear prevalence that, on average, was not significant (13).…”

Polyurethane derived from Ricinus Communis as graft for bone defect treatments

“…Leite and Ramalho [10] developed a study to compare the differences in the reaction of the connective tissue and the jaw bone of rats when exposed to this resin and demineralized bovine bone matrix. The results indicate biocompatibility of both materials, considering their integration into the rat jaw, and demonstrating that polyurethane is an alternative in bone reconstruction with the advantage of being an inexhaustible source of biomaterial.…”

Can Thermal Water Be an Alternative Treatment Method for Wound Healing?

“…There is a balance between collagen production and destruction. Inflammatory cells gradually decrease (Leite & Ramalho, 2008). In addition, the most important chemotactic factor for fibroblasts in the proliferation phase is PDGF.…”