Humoral immune response to antigens of Porphyromonas gingivalis ATCC 33277 in chronic periodontitis

Abstract

Introduction:Periodontitis is a chronic disease that results from an interaction of a mixed bacterial challenge and the host response.Objective:The purposes of this study were to evaluate the IgG serum levels to Porphyromonas gingivalis antigens by ELISA in individuals with different periodontal conditions correlated with clinical parameters, and to analyze the immunoreactivity profiles by Western blotting.Methods:Serum IgG levels against the cell sonicate antigen from P. gingivalis ATCC 33277 of 28 patients with chronic periodontitis (CP), 10 patients with gingivitis (G) and 21 periodontally healthy individuals (H) were measured by ELISA and Western immunoblotting.Results:In the CP group, sera reactivity by ELISA was significantly higher than in the G and H groups (Kruskal-Wallis p<0.001; Dunnet t3 p= 0.001 and Dunnet t3 p= 0.0001). There was no statistically significant difference between G and HP reactivity (Dunnett t3 p=0.617). Among individuals with chronic periodontitis, the IgG-anti-P. gingivalis serum levels were positively correlated with percentage of clinical attachment level =5mm (rs = + 0.375, p<0.05) and a negative correlation was found between IgG-anti-P. gingivalis levels and percentage of probing pocket depth 0-3mm (rs = - 0. 411, p< 0.05). The analysis of sera immunoreactivity profiles to sonicate antigen by Western blotting showed differences between the sera of CP, G and H group individuals. The serum from CP frequently reacted with high molecular weight (103 kDa, 86 kDa, 72 kDa, 60 kDa, 58 kDa, 52 kDa) protein fractions.Conclusions:Serum levels of IgG anti-P. gingivalis distinguished individuals with chronic periodontitis, gingivitis and healthy periodontium. There was a correlation between clinical parameters and serum IgG levels against P. gingivalis. There was a difference in the recognition profile of protein fractions among the studied groups and some bands were more specific

Association between Periodontitis and Severe Asthma: The Role of IgG Anti-Porphyromonas gingivalis Levels

“…Another relevant result was similar production levels of IgG specific to P. gingivalis among the participants, regardless of whether they had severe asthma, periodontitis, or neither of these conditions. While the literature indicates that individuals with periodontitis are expected to harbor significantly higher levels of IgG specific to Porphyromonas gingivalis (17) , the present results were divergent. This would seem to suggest that the presence of severe asthma may influence the humoral response to Porphyromonas gingivalis, the relative quantities of Porphyromonas gingivalis were found to be significantly lower in the subgingival biofilm of individuals with chronicperiodontitis.…”

“…Confirming the role of Porphyromonas gingivalisin the etiology and pathogenesis of periodontitis, high levels of specific IgG against this bacteria were found in the sera of affected individuals (17,18) . Moreover, individuals with chronic periodontitis presented higher levels of IgG against Porphyromonas gingivalis antigens, such as HmuY and the gingipains proteinases, than healthy individuals, or those with gingivitis (17)(18)(19)(20)(21) .…”

Different engagement of TLR2 and TLR4 in Porphyromonas gingivalis vs. ligature-induced periodontal bone loss

“…27,28 In the present study, P. gingivalis infection showed significant increases in periodontal bone resorption in WT mice and TLR2 KO mice but not in TLR4 KO mice and TLR2&4 KO mice (Figure 2A-D), suggesting that TLR4 is essential for bone loss associated with P. gingivalis infection. However, RANKL protein levels were significantly higher in the P. gingivalis infection group in TLR4 KO mice (Figure 3G) and showed no significant change in TLR2&4 KO mice (Figure 3H), indicating that not only TLR4 signaling but also TLR2 signaling is involved in the RANKL-mediated bone loss.…”

Autoantibodies specific to a peptide of β2-glycoprotein I cross-react with TLR4, inducing a proinflammatory phenotype in endothelial cells and monocytes

“…33 The cells of P gingivalis, kindly provided by Dr P. Mastrantonio (Istituto Superiore di Sanità, Rome, Italy), were resuspended in PBS containing a protease inhibitor cocktail diluted 1:1000 (P-8465; Sigma-Aldrich) and subjected to ultrasonic disruption at output 3 in a sonifier (Bronson Sonic Power) for 20 minutes.…”