Comparative evaluation of antimicrobial action of MTA, calcium hydroxide and Portland cement

Ribeiro, Caroline Sousa; Kuteken, Fernanda Akemi; Júnior, Raphael Hirata; Scelza, Míriam Zaccaro

doi:10.1590/s1678-77572006000500006

Cited by 23 publications

(24 citation statements)

References 12 publications

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“…However, in this study all materials were ineffective against E. faecalis, as similar to those observed in the literature related to Estrela et al 4 , Ribeiro et al 13 , Zarrabi et al 14 and Miyagak et al 24 , in constrast to the results presented in Sipert et al 11 and Tanumaru Filho et al 12 investigations.…”

Section: Discussionsupporting

confidence: 92%

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Evaluation of cariogenic antibacterial activity of mineral trioxide aggregate and Portland cement

Júnior

Sobral

Sampaio

et al. 2015

RGO, Rev. Gaúch. Odontol.

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ObjectiveThe objective of this study was to evaluate the antimicrobial activity of Mineral trioxide aggregate e Portland cement against some selected cariogenic bacteria. Methods Wells were made of approximately 0.5 mm in diameter, in solid culture media and immediately filled with cement. Twelve samples of each material were obtained for the realization of the agar diffusion method. These samples were tested with Streptococcus mutans (ATCC 700610), Enterococcus faecalis (ATCC 29212), Lactobacillus acidophilus (UFV) and Lactobacillus casei (UNICAMP). Petri plates containing Tripticase soy Agar (TSA) were used for the growth of Streptococcus mutans and Enterococcus faecalis, and plates of Man Rogosa & Sharpe Agar (MRS) for the Lactobacillus acidophilus and Lactobacillus casei. After 48 hours of incubation, it was made the measurement of inhibition halos with the aid of manual caliper. ResultsThe diameters obtained to MTA (2.50 ± 0.00;2,92 ± 0.63; 2.58 ± 0.38), PC (1.17 ± 0.29; 2.00 ± 0.43; 1.33 ± 0.14) and calcium hydroxide cement (3.83 ± 0.29; 3.00 ± 0.00 ± 2.58 ± 0.72) ResultadosOs diâmetros obtidos do MTA (2.50 ± 0.00; 2,92 ± 0.63; 2.58 ± 0.38), CP (1.17 ± 0.29; 2.00 ± 0.43; 1.33 ± 0.14) e cimento de hidróxido de cálcio (3.83 ± 0.29; 3.00 ± 0.00 ± 2.58 ± 0.72) foram expressos em milímetros e submetidos ao teste de Tukey (p<0.05). Conclusão METHODSThe tested materials were: MTA (Angelus Soluções Odontológicas, Londrina, Brasil), Portland cement (CP II -F32, ITAPESSOCA AGRO-INDUSTRIAL S.A., Goiana, Brasil) and calcium hydroxide paste (Reagen TM).The antimicrobial activity of the materials was evaluated by the Agar diffusion method against four different strains.The strains used for analysis were Streptococcus mutans (ATCC 700610), Enterococcus faecalis (ATCC 29212), Lactobacillus acidophilus and Lactobacillus casei isolated by the Laboratory of Microbiologic Science (Federal University of Viçosa -MG (UFV) and University State of Campinas -UNICAMP, SP, Brazil), respectively.Of each bacterial culture tested, it was held a concentration suspension of approximately 5x108 of colonies per mL, corresponding to 0.5 of Mc Farland em range of sterile medium Trypticase Soy Browth (TSBMerck). The Streptococcus mutans and Enterococcus faecalis suspensions were inoculated with spread plate in the middle of Sodium Tioglicolate (Merck) culture and Lactobacillus and Lactobacillus acidophilus casei were inoculated in the culture medium Man Rogosa & Sharpe Agar (MRS-HIMEDIA). Subsequently, the plates were incubated at 35 ± 1°C for 24/48h and the halos of inhibition were expressed in millimeters, using a caliper manual.A total of 12 plates were employed, each microorganism was tested in triplicate. Three cavities, measuring 4mm in diameter were made in each agar plate using a copper puncher and then completely filled with freshly manipulated product to be tested.Positive (CaOH) and negative controls (sterile growing medium) were tested using the same methodology and under the same conditions of the experiment.All cult...

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Section: Discussionsupporting

confidence: 92%

“…The antimicrobial effect of MTA and PC measured the test of inhibition in agar was already observed against microorganisms [13][14][15] . However, no previous studies have evaluated the antimicrobial activity exclusively against bacterial strains of deep decayed dentin [16][17] .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of cariogenic antibacterial activity of mineral trioxide aggregate and Portland cement

Júnior

Sobral

Sampaio

et al. 2015

RGO, Rev. Gaúch. Odontol.

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“…This result is similar with previous study 8,10,13,16 This variation in antimicrobial activity between MTA and Ca(OH)2 may be due to different diffusion and dissociation capabilities of two materials. Some substances have difficulty in dissociating and diffusing in agar (semi-solid medium), not expressing their real antimicrobial effect.…”

Section: Comparison Of Zone Of Inhibition Between Mta and Calcium Hydsupporting

confidence: 93%

“…8,13,16 However, there is not a consensus regarding to a gold standard test for the appraisal of antimicrobial testing of cements and other solutions used in dental therapy.…”

Section: Comparison Of Zone Of Inhibition Between Mta and Calcium Hydmentioning

confidence: 99%

Antimicrobial Efficacy of Mineral Trioxide Aggregate against Therapy Resistant Endodontic Microorganisms

Banu¹,

Bashar

Howlader

et al. 2016

J. Bangladesh Coll. Phys.

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Microorganisms, usually from the dental caries, are the main sources of diseases in dental pulp (root canals) and periapical region. Facultative bacteria and fungi have been identified in therapy resistant persistent endodontic infection. The objectives of this study was to evaluate the antimicrobial efficacy of Mineral Tri Oxide Aggregate (MTA) against therapy resistant endodontic microorganisms. The efficacy of MTA was also compared with that of calcium hydroxide. Six standard bacterial stains were used: Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, bacillus subtilis, Candida albicans and Enterococcus faecalis. The agar diffusion method on MullerHilton media was employed. The plates containing media were inoculated with the specified bacterial suspensions. Two standard holes were prepared on each microorganism inoculated plate with a copper puncher and one hole was completely filled with MTA & the other with Ca (OH) 2 . The plates were then kept at environmental temperature for one hour to ensure prediffusion and then incubated at 37 0 C for 24 hours. After 24 hours, the diameters of inhibition zones were measured. Tests were replicated for thirty times for each sample and mean values were taken. Zone of inhibition as measured for MTA and Ca (OH) 2 were statistically analyzed with Student's t-Test and Post Hoc Games Howell Test and were presented as mean ± SD to compare of efficacy of MTA and calcium hydroxide on different microorganisms. Both MTA and Ca(OH) 2 were found to produce zone of inhibition against Staphylococcus aureus (ATCC 25923), Pseudomonas aeruginosa (ATCC 27853), , Bacillus subtilis (BTCC 17 ), and Candida albicans (BTCC 493). MTA showed highest activity against S. aureus and lowest activity against P. aeruginosa which was similar to the activity range of Ca (OH) 2 against the mentioned organisms. But both of them failed to produce any activity against E. coli and. E. faecalis. MTA was found to produce a lower efficacy than Ca (OH) 2 while comparing the zone of inhibition between them and statistically it was significant. Mineral Tri Oxide Aggregate (MTA) showed antimicrobial efficacy against some therapy resistant microorganisms but it did not show antimicrobial efficacy against Escherichia coli and Enterococcus faecalis. MTA was found to produce a lower antimicrobial efficacy than Ca (OH) 2 .

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“…In another study, Ribeiro et al 20 demonstrated that the antibacterial activity of MTA against Escherichia coli under aerobic conditions was promoted by the induction of reactive oxygen species. In another study, Ribeiro et al 21 found that E. faecalis was susceptible to grey MTA, as well as calcium hydroxide, after incubation in aerobic conditions. They proposed that an oxygen-enriched medium favours the antimicrobial activity of MTA.…”

Section: Discussionmentioning

confidence: 94%

Antibacterial activity of a new mineral trioxide aggregate-based root canal sealer

Mohammadi

Giardino

Palazzi

et al. 2012

International Dental Journal

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Comparative evaluation of antimicrobial action of MTA, calcium hydroxide and Portland cement

Cited by 23 publications

References 12 publications

Evaluation of cariogenic antibacterial activity of mineral trioxide aggregate and Portland cement

Evaluation of cariogenic antibacterial activity of mineral trioxide aggregate and Portland cement

Antimicrobial Efficacy of Mineral Trioxide Aggregate against Therapy Resistant Endodontic Microorganisms

Antibacterial activity of a new mineral trioxide aggregate-based root canal sealer

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