Biocompatibility of acellular dermal matrix graft evaluated in culture of murine macrophages

This study uses high-resolution ultrasound to examine growth and development of engineered oral mucosal tissues manufactured under aseptic conditions. The specimens are a commercially available natural tissue scaffold, AlloDerm® and oral keratinocytes seeded onto AlloDerm® to form a ex vivo produced oral mucosal equivalent (EVPOME) suitable for intraoral grafting. The seeded cells produce a keratinized protective upper layer which smooth’s out any remaining surface irregularities on the underlying AlloDerm®. Two-dimensional acoustic imaging of unseeded AlloDerm® and developing EVPOMEs were performed at each consecutive day of their growth and development, requiring imaging each of the tissue specimens under aseptic conditions (total time from seeding to maturation: 11 days). Ultrasonic monitoring offers us an ability to determine the constituents in the EVPOME that are responsible for changes in its mechanical behavior during the manufacturing process. Ultrasonic monitoring affords us an opportunity to non-invasively assess, in real time, tissue engineered constructs prior to release for use in patient care.

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High-Frequency Ultrasonic Imaging of Growth and Development in Manufactured Engineered Oral Mucosal Tissue Surfaces

Winterroth

Kato

Kuo

et al. 2014

Ultrasound in Medicine & Biology

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Effects of a probiotic soy product and physical exercise on formation of pre-neoplastic lesions in rat colons in a short-term model of carcinogenic

Silva

Sivieri

Rossi

2009

Journal of the International Society of Sports Nutrition

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Purpose: In this study the influence of moderate or intense physical exercise, alone or in combination with the consumption of a soya product fermented with Enterococcus faecium, on the development of colon cancer induced chemically in rats with 1,2-dimethylhydrazine (DMH), was investigated.Methods: Eighty male Wistar SPF rats were randomly allocated to 8 groups (n = 10). One week after the start of the program of product ingestion and/or physical activity, all animals except the controls (group I) were injected subcutaneously with 50 mg/kg b.w. of 1,2-dimethylhydrazine (DMH). This procedure was repeated at the end of the second week. At the end of the 6-week experiment, all the animals were euthanized; the colons were removed and numbers of ACF was estimated.Results: Twenty-four days after the induction of pre-neoplastic lesions, it was evident that the formation of ACF was not significantly reduced by the ingestion of the fermented product, by intense or moderate physical activity or by a combination of these factors, in comparison with the positive control group of rats (p < 0.05). On the other hand, the performance of intense exercise, on its own, increased the number of ACF. Conclusion:The results reported in this article show that consumption of the fermented soy product described here and the practice of physical exercise (intense or moderate) were incapable, separately or combined, of inhibiting the formation of ACF in DMH-induced rats. The intense physical exercise led to an increased number of foci in the colons of these rats and, probably, to greater susceptibility to colorectal cancer.

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Biocompatibility of acellular dermal matrix graft evaluated in culture of murine macrophages

Cited by 2 publications

References 19 publications

High-Frequency Ultrasonic Imaging of Growth and Development in Manufactured Engineered Oral Mucosal Tissue Surfaces

High-Frequency Ultrasonic Imaging of Growth and Development in Manufactured Engineered Oral Mucosal Tissue Surfaces

Effects of a probiotic soy product and physical exercise on formation of pre-neoplastic lesions in rat colons in a short-term model of carcinogenic

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