Tetrazolium test for Pinus taeda: preparation, staining, and seed viability classes

Santos, Felipe Serrato dos; Vieira, Elisa Serra Negra; Panobianco, Maristela

doi:10.1590/s1678-3921.pab2019.v54.01088

Cited by 4 publications

(5 citation statements)

References 5 publications

(4 reference statements)

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“…The pattern found, which describes the highest percentage of viable seeds in the maximum staining time (3 hours), is due to the fact that it is a batch of viable and vigorous seeds. This result is in accordance with the study presented by (dos Santos et al, 2019) on the viability and vigor of Genipa americana seeds, which showed that increasing the staining time of the seeds produces higher percentages of viable seeds. In our experiment, it was observed that O. pyramidale requires a longer staining time to show high viability, so it would appear that this is the appropriate protocol to analyze the viability of seeds in this species.…”

Section: Discussionsupporting

confidence: 93%

“…In our experiment, it was observed that O. pyramidale requires a longer staining time to show high viability, so it would appear that this is the appropriate protocol to analyze the viability of seeds in this species. dos Santos et al, 2019;Espitia-Camacho et al, 2017;Jácome-Segovia et al, (2019), carried out seed viability studies in forest species to determine the favorable response in terms of concentration and staining time in tetrazolium.…”

Section: Discussionmentioning

confidence: 99%

“…With the treatment of seeds submitted to the last staining time (3 hours), seed vigor was determined in levels (high, medium and low), based on the methodology proposed by (dos Santos et al, 2019), which facilitated the selection of viable and non-viable seeds expressed as a percentage, as follows.…”

Section: Seed Viabilitymentioning

confidence: 99%

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Seed viability, germination and seedling quality patterns of three forest species for restoration in Amazonian conditions

et al. 2023

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The aim of this study was to evaluate the quality patterns of Switenia macrophylla, Cedrelinga cateniformis and Ochroma pyramidale seeds and seedlings in response to restoration. The seed viability test was performed using 1 % tetrazolium (2,3,5-triphenyl tetrazolium chloride) during three staining times and the percentage of viability and vigor levels were considered according to the topological staining pattern. Germination speed, germination capacity, and morphological indices of plant quality were determined using the aboveground belowground ratio, slenderness, degree of lignification and Dickson's quality. The highest percentage of viable seeds with the maximum staining time (3 hours) was verified. The medium vigor category was higher in the three species, as determined by a partial reddish staining pattern as an expression of their physiological quality, which was reflected in the response to germination. The morphological quality indices allowed us to identify the species’ potential for growth and development for acclimatization to the area. The correspondence analysis was significant (p≤0.05), which facilitated the formation of quality groups, C. cateniformis was found to be of a high-enough quality as an indicator of its potential to cover restoration needs in Amazonian conditions.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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Seed viability, germination and seedling quality patterns of three forest species for restoration in Amazonian conditions

et al. 2023

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“…Accordingly, they were classified as viable and non-viable embryos, respectively, observing the following order: Class 1viable and vigorous embryos (Figure 4 ease of identification for viable and non-viable tissues and in its ability to differentiate seed vigor. The colors differences observed in the seeds after staining in tetrazolium solution are the main features to be considered in the interpretation of results of specific tests (Santos, Vieira, & Panobianco, 2019).…”

Section: Resultsmentioning

confidence: 99%

Suitability of tetrazolium test for Tamarindus indica L. seeds

Cordeiro

Bruno

Lima

et al. 2022

Semina: Ciênc. Agrár.

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Seed quality is routinely assessed by direct tests, e.g, the germination test, or indirect tests like the tetrazolium test, which has shoown to be promising in the determine viability and vigor, allowing the diagnosis of the main problems that may affect seed quality, such as mechanic damages, field deterioration and storage. In this respect, this study was conducted to develop a tetrazolium test protocol to evaluate the viability and vigor of Tamarindus indica L. seeds. Before exposing the seeds to the tetrazolium solution, seed preconditioning studies were carried out in which seven soaking times were tested. The soaking time that did not cause damage to the seed embryo and allowed the removal of the seed coat to expose the seed structures to the tetrazolium salt was selected. Then, an experiment was set up in a completely randomized design with a 2x3x3 factorial arrangement involving two seed lots, three soaking times in tetrazolium salt (6, 12 and 16 h) and three salt concentrations (0.075, 0.1 and 0.5%), totaling 18 treatments with four replicates of 25 seeds, evaluated at 40 ºC. For each treatment, the seeds were divided into three classes, namely, viable and vigorous embryos (class 1); viable embryos (class 2) and non-viable or dead embryos (class 3). For a comparison with the tetrazolium test results, the two seed lots were analyzed for water content, germination, emergence, first count, germination speed index, emergence speed index, growth and seedling dry weight. The viability and vigor of T. indica seeds can be evaluated after a soaking period of 48 h and immersion for 6h in tetrazolium salt at the concentration of 0.1%, at 40°C, with provides results similar to conventional seed viability tests. The tetrazolium test proved to be adequate to differentiate T. indica seed lots in terms of viability.

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“…Some methods are used to determine the physiological quality of seeds of the genus Pinus, such as the spectroscopy-based reserve metabolite profiling analysis (Terskikh et al, 2005), which is still not viable on large scale, and the tetrazolium test, especially for the P. taeda species (ISTA, 2020;Santos et al, 2019). The germination test, however, is the only standard test available to determine the viability of P. taeda seeds (ISTA, 2020).…”

Section: Introductionmentioning

confidence: 99%

Time reduction to evaluate Pinus taeda seeds germination by time-to-event analysis

Michelon

Santos

Belniaki

et al. 2022

Sci. agric. (Piracicaba, Braz.)

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Tetrazolium test for Pinus taeda: preparation, staining, and seed viability classes

Cited by 4 publications

References 5 publications

Seed viability, germination and seedling quality patterns of three forest species for restoration in Amazonian conditions

Seed viability, germination and seedling quality patterns of three forest species for restoration in Amazonian conditions

Suitability of tetrazolium test for Tamarindus indica L. seeds

Time reduction to evaluate Pinus taeda seeds germination by time-to-event analysis

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