Lytic bacteriophages as a potential alternative to control Staphylococcus aureus

Leite, Juliana Almeida; Pereira, Hyago Passe; Borges, Cristiano Amâncio Vieira; Alves, Bruno Henrique; Ramos, Alessandra Isis Alves Pinheiro; Martins, Marta Fonseca; Arcuri, Edna Froeder

doi:10.1590/s1678-3921.pab2019.v54.00917

Cited by 8 publications

(6 citation statements)

References 25 publications

(38 reference statements)

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“…Other authors have shown similar restriction profiles among phages isolated from different environments [ 40 , 41 ]. Similar results were observed by Leite et al [ 42 ], who also did not observe a correlation between the genetic relationships among six S. aureus phages, determined based on restriction analysis, and their lytic activity.…”

Section: Discussionsupporting

confidence: 90%

Bacteriophages for Controlling Staphylococcus spp. Pathogens on Dairy Cattle Farms: In Vitro Assessment

Pyzik,

Urban-Chmiel,

Kurek

et al. 2024

Animals

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Pathogenic Staphylococcus spp. strains are significant agents involved in mastitis and in skin and limb infections in dairy cattle. The aim of this study was to assess the antibacterial effectiveness of bacteriophages isolated from dairy cattle housing as potential tools for maintaining environmental homeostasis. The research will contribute to the use of phages as alternatives to antibiotics. The material was 56 samples obtained from dairy cows with signs of limb and hoof injuries. Staphylococcus species were identified by phenotypic, MALDI-TOF MS and PCR methods. Antibiotic resistance was determined by the disc diffusion method. Phages were isolated from cattle housing systems. Phage activity (plaque forming units, PFU/mL) was determined on double-layer agar plates. Morphology was examined using TEM microscopy, and molecular characteristics were determined with PCR. Among 52 strains of Staphylococcus spp., 16 were used as hosts for bacteriophages. Nearly all isolates (94%, 15/16) showed resistance to neomycin, and 87% were resistant to spectinomycin. Cefuroxime and vancomycin were the most effective antibiotics. On the basis of their morphology, bacteriophages were identified as class Caudoviricetes, formerly Caudovirales, families Myoviridae-like (6), and Siphoviridae-like (9). Three bacteriophages of the family Myoviridae-like, with the broadest spectrum of activity, were used for further analysis. This study showed a wide spectrum of activity against the Staphylococcus spp. strains tested. The positive results indicate that bacteriophages can be used to improve the welfare of cattle.

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Section: Discussionsupporting

confidence: 90%

Bacteriophages for Controlling Staphylococcus spp. Pathogens on Dairy Cattle Farms: In Vitro Assessment

Pyzik,

Urban-Chmiel,

Kurek

et al. 2024

Animals

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“…Generally, phages of most S. aureus strains interact with a unique cell wall teichoic acid, which is different from other coagulase-negative staphylococci (CoNS) and blocks recognition by phages specific to CoNS using the tail tip complex [ 74 ]. For studies specifically conducted on bovine mastitis, S. aureus phages utilise three domains located on the endolysin sequences: cysteine; histidine-dependent amidohydrolase/peptidase (CHAP); amidase 2 (N-acetylmuramyl-L-alanine amidase); and SH3b for host cell wall recognition [ 42 ].…”

Section: The Case For Phage Therapy To Control Bovine Mastitismentioning

confidence: 99%

Bacteriophage Therapy to Control Bovine Mastitis: A Review

Nale

McEwan

2023

Antibiotics

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Bovine mastitis is a polymicrobial disease characterised by inflammation of the udders of dairy and beef cattle. The infection has huge implications to health and welfare of animals, impacting milk and beef production and costing up to EUR 32 billion annually to the dairy industry, globally. Bacterial communities associated with the disease include representative species from Staphylococcus, Streptococcus, Enterococcus, Actinomyces, Aerococcus, Escherichia, Klebsiella and Proteus. Conventional treatment relies on antibiotics, but antimicrobial resistance, declining antibiotic innovations and biofilm production negatively impact therapeutic efficacy. Bacteriophages (phages) are viruses which effectively target and lyse bacteria with extreme specificity and can be a valuable supplement or replacement to antibiotics for bovine mastitis. In this review, we provide an overview of the etiology of bovine mastitis, the advantages of phage therapy over chemical antibiotics for the strains and research work conducted in the area in various model systems to support phage deployment in the dairy industry. We emphasise work on phage isolation procedures from samples obtained from mastitic and non-mastitic sources, characterisation and efficacy testing of single and multiple phages as standalone treatments or adjuncts to probiotics in various in vitro, ex vivo and in vivo bovine mastitis infection models. Furthermore, we highlight the areas where improvements can be made with focus on phage cocktail optimisation, formulation, and genetic engineering to improve delivery, stability, efficacy, and safety in cattle. Phage therapy is becoming more attractive in clinical medicine and agriculture and thus, could mitigate the impending catastrophe of antimicrobial resistance in the dairy sector.

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“…A utilização da terapia bacteriofágica é relativamente antiga, foi descoberta Research, Society andDevelopment, v. 10, n. 2, e40310212546, 2021 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v10i2.12546 por Francis Twort em 1915 e Felix d´Herelle em 1917, que verificaram agentes terapêuticos potenciais, quando examinou atividade lítica dos bacteriófagos em bactérias específicas (D´HERELLE, 1931como citado em FANIN et al, 2020. Leite et al (2019) isolaram dois bacteriófagos de estábulos que apresentaram uma alta atividade lítica em S. aureus, em ampla gama de estirpes, o que indicou seu potencial para estudos de fagoterapia em gado leiteiro ou como agente de controle biológico para produtos lácteos.…”

Section: Utilização De Métodos Alternativos No Controle Do Desenvolviunclassified

Uso de diferentes métodos no controle do desenvolvimento do Staphylococcus aureus: uma revisão da literatura

Freitas

Lima

Coelho

et al. 2021

RSD

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Staphylococcus aureus é uma bactéria patogênica que pode causar várias doenças em seres humanos e animais. O uso de antibióticos no controle do seu desenvolvimento tem sido muitas vezes ineficiente tendo em vista sua capacidade de tornar-se resistente a estas substâncias. Assim, a busca por novas alternativas eficazes no combate deste microrganismo é um desafio para ciência e tem se tornando extremamente relevante. Este estudo objetivou analisar na literatura o uso de diferentes substâncias, naturais e químicas, no controle do desenvolvimento do S. aureus isolados de diferentes fontes. Para composição deste estudo foram utilizados livros, dissertações, teses e artigos científicos das bases de dados Google acadêmico, Scielo e Science direct. Os trabalhos foram selecionados de forma austera, conforme o conteúdo do título e/ou resumo. Com base nos resultados concluiu-se que o uso de antibióticos, apesar de ser uma forma eficiente para o controle do desenvolvimento do S. aureus, seu uso deve ser controlado em virtude da possível ocorrência de cepas resistentes a estas substancias. Outras possibilidades para o controle desse microrganismo têm apresentado eficiência, como o uso de óleos essenciais, extrato de plantas, biofilmes, filmes a base de gelatina, controle biológico por bacteriófagos líticos e ozonioterapia. Contudo, para uso destas substâncias, maiores estudos devem ser realizados.

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Lytic bacteriophages as a potential alternative to control Staphylococcus aureus

Cited by 8 publications

References 25 publications

Bacteriophages for Controlling Staphylococcus spp. Pathogens on Dairy Cattle Farms: In Vitro Assessment

Bacteriophages for Controlling Staphylococcus spp. Pathogens on Dairy Cattle Farms: In Vitro Assessment

Bacteriophage Therapy to Control Bovine Mastitis: A Review

Uso de diferentes métodos no controle do desenvolvimento do Staphylococcus aureus: uma revisão da literatura

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