Rapid thawing human sperm does not affect basic parameters in normozoospermic men: a double-blind prospective study

Vieira, Marco A; Nery, Simone F.; Tavares, Ricardo; Cruz, Cynthia Dela; Reis, Fernando M.; Camargos, Aroldo F.

doi:10.1590/s1677-55382012000100015

Cited by 6 publications

(2 citation statements)

References 14 publications

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“…It is widely accepted that sperm viability is affected by cryopreservation. As confirmation to this notion, a decrease in viable cells (vitality index = 50%) has been reported in samples from proven fertile and subfertile men ( Vieira et al ., 2012 ). Interestingly, outcomes appear to improve when rapid cryopreservation protocols are used in comparison to slow freezing methods (64.8% vs. 50.4%) ( Vutyavanich et al ., 2010 ).…”

Section: Discussionsupporting

confidence: 64%

A retrospective study on sperm banking: a Uruguayan experience

Ordoqui¹,

Barrera²,

Montes³

et al. 2018

JBRA Assisted Reproduction

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ObjectiveThe purpose of this study was to investigate the status of homologous sperm banking in Uruguay.MethodsA retrospective investigation was performed on data collected between 2013 and 2015. Reasons for sperm banking, patient age, pre-freeze and post-thaw semen parameters, and recovery rates were analyzed.Results623 samples were cryobanked between 2013 and 2015. Only 324 samples were considered for analysis after selection based on inclusion criteria. In most cases the samples were stored because the patients were undergoing assisted reproductive technology (ART) treatment (n=190; 58,64%) or for oncological reasons (n=113; 34,88%). The median age of bankers was 34 years. In the cancer group, 61.95% (n=70) of the subjects had been diagnosed with testicular cancer. Medians of semen parameters for both groups were above the lower reference limits dictated by the World Health Organization (2010). In fresh samples, a significant difference was observed in progressive motility (47% vs. 56%) between ART and oncological patients. After thawing, total motility (27% vs. 32%), progressive motility (19% vs. 22%), and vitality (48% vs. 56%) differed significantly between ART and oncological bankers.ConclusionSemen banking has been performed successfully in Uruguay and outcomes are on par with international standards. Surprisingly, the semen parameters of the cancer group were nearly normal.

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Section: Discussionsupporting

confidence: 64%

A retrospective study on sperm banking: a Uruguayan experience

Ordoqui¹,

Barrera²,

Montes³

et al. 2018

JBRA Assisted Reproduction

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“…in viable cells after cryopreservation, even in fertile men (Vieira et al, 2012). Our post-thaw viability recovery rate (76%) is in agreement with values expected and reported in the literature (Counsel, Bellinge, & Burton, 2004;Ordoqui et al, 2018;Verheyen et al, 1993;World Health Organization, 2010).…”

Section: Discussionsupporting

confidence: 91%

The Uruguayan semen donor population: A twenty‐eight‐year retrospective study

Barrera¹,

Ordoqui²,

Montes³

et al. 2019

Andrologia

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Several studies have reported a global decline in seminal quality over the years. The objective of this study was to describe the semen donor population of Uruguay through comparing data of successive samples banked by the same donors and the analysis of their semen and physical characteristics, ancestry origin and educational level. A total of 3,449 ejaculated samples collected from 71 donors, cryobanked between 1989 and March 2017 at Fertilab, were analysed. Results revealed a mean age of 23.90 ± 3.98 years, an average weight of 74.95 ± 1.09 kg and a mean height of 1.78 ± 0.06 m. The majority of the donors trace their origin to Europe (74.65%, 53/71) and 66.19% (47/71) have a level of education higher than secondary school.We observed longitudinal differences in two parameters, that is sperm concentration and semen volume. Sperm concentration declined, while semen volume increased significantly over the 28-year period. The results of the present study are in accordance with that of previous articles that also reported a decline in sperm concentration over time. However, no differences were observed in total sperm number per ejaculate due to the increase in semen volume values, thus reflecting no real changes in sperm production over time. K E Y W O R D Sbanking, cryopreservation, donor, spermatozoa

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Cryopreservation of Marchantia polymorpha spermatozoa

et al. 2018

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The liverwort Marchantia polymorpha has become one of the model organisms, since it has less genetic redundancy, sexual and asexual modes of reproduction and a range of genomic and molecular genetic resources. Cryopreservation of fertile spermatozoa eliminates time, space and labor for growing and maintaining male plants in reproductive phase, and also provides an optional way to backup lines. Here we report a protocol to cryopreserve spermatozoa of M. polymorpha in liquid nitrogen. A cryoprotective solution containing sucrose, glycerol and egg yolk and controlled cooling and warming processes led to successful recovery of motile M. polymorpha spermatozoa after the cryogenic process. The survival rate and average motility of spermatozoa after cryopreservation were maintained at 71 and 54% of those before cryopreservation, respectively. Cryopreserved spermatozoa were capable of fertilization to form normal spores. The technique presented here confers more versatility to experiments using M. polymorpha and could be applied to preservation of plant spermatozoa in general.

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Rapid thawing human sperm does not affect basic parameters in normozoospermic men: a double-blind prospective study

Abstract: ARTICLE INFO___________________________________________ _

Cited by 6 publications

References 14 publications

A retrospective study on sperm banking: a Uruguayan experience

A retrospective study on sperm banking: a Uruguayan experience

The Uruguayan semen donor population: A twenty‐eight‐year retrospective study

Cryopreservation of Marchantia polymorpha spermatozoa

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Rapid thawing human sperm does not affect basic parameters in normozoospermic men: a double-blind prospective study

Abstract: ARTICLE INFO_______________________________________________________________ _____________________

Cited by 6 publications

References 14 publications

A retrospective study on sperm banking: a Uruguayan experience

A retrospective study on sperm banking: a Uruguayan experience

The Uruguayan semen donor population: A twenty‐eight‐year retrospective study

Cryopreservation of Marchantia polymorpha spermatozoa

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Product

Resources

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Abstract: ARTICLE INFO___________________________________________ _